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Inactivation of different strains of Escherichia coli O157:H7 in various apple ciders treated with dimethyl dicarbonate (DMDC) and sulfur dioxide (SO2) as an alternative method.
Food Microbiol. 2009 Feb; 26(1):8-15.FM

Abstract

Escherichia coli has been identified as the causative agent in numerous foodborne illness outbreaks associated with the consumption of fresh apple cider. Apple cider has a pH which is normally below 4.0 and would not be considered a medium capable of supporting the growth of foodborne pathogens. The association of unpasteurized apple cider with foodborne illness due to E. coli O157:H7 has however, led to increased interest in potential alternative methods to produce pathogen free cider. Apple cider was prepared from eight different apple cultivars, inoculated with approximately 10(6)-10(7) CFU of three strains of E. coli O157:H7 per ml (933, ATCC 43889, and ATCC 43895) and tested to determine the effectiveness of sulfur dioxide (SO(2)) and dimethyl dicarbonate (DMDC). Bacterial populations for treated and untreated samples were then enumerated by using non-selective media. Eight different ciders were treated with DMDC (125 and 250 ppm) and SO(2) (25, 50, 75, 100 ppm). Greater than a 5-log reduction was achieved at room temperature with 250 ppm of DMDC and 50 ppm of SO(2) after the incubation time of 6h and 24h, respectively. Addition of DMDC and/or SO(2) may offer an inexpensive alternative to thermal pasteurization for the production of safe apple cider for small apple cider producers.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Basaran-Akgul N
Department of Food Science and Human Nutrition, Washington State University, Pullman, Washington 99164-6376, USA.
Churey JJ
No affiliation info available
Basaran P
No affiliation info available
Worobo RW
No affiliation info available

MeSH

BeveragesColony Count, MicrobialConsumer Product SafetyDeoxycytidineDose-Response Relationship, DrugEscherichia coli O157Food HandlingFood MicrobiologyFood PreservationFood PreservativesHumansHydrogen-Ion ConcentrationMalusSulfur DioxideTemperatureTime Factors

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19028298

Citation

Basaran-Akgul, N, et al. "Inactivation of Different Strains of Escherichia Coli O157:H7 in Various Apple Ciders Treated With Dimethyl Dicarbonate (DMDC) and Sulfur Dioxide (SO2) as an Alternative Method." Food Microbiology, vol. 26, no. 1, 2009, pp. 8-15.
Basaran-Akgul N, Churey JJ, Basaran P, et al. Inactivation of different strains of Escherichia coli O157:H7 in various apple ciders treated with dimethyl dicarbonate (DMDC) and sulfur dioxide (SO2) as an alternative method. Food Microbiol. 2009;26(1):8-15.
Basaran-Akgul, N., Churey, J. J., Basaran, P., & Worobo, R. W. (2009). Inactivation of different strains of Escherichia coli O157:H7 in various apple ciders treated with dimethyl dicarbonate (DMDC) and sulfur dioxide (SO2) as an alternative method. Food Microbiology, 26(1), 8-15. https://doi.org/10.1016/j.fm.2008.07.011
Basaran-Akgul N, et al. Inactivation of Different Strains of Escherichia Coli O157:H7 in Various Apple Ciders Treated With Dimethyl Dicarbonate (DMDC) and Sulfur Dioxide (SO2) as an Alternative Method. Food Microbiol. 2009;26(1):8-15. PubMed PMID: 19028298.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inactivation of different strains of Escherichia coli O157:H7 in various apple ciders treated with dimethyl dicarbonate (DMDC) and sulfur dioxide (SO2) as an alternative method. AU - Basaran-Akgul,N, AU - Churey,J J, AU - Basaran,P, AU - Worobo,R W, Y1 - 2008/08/22/ PY - 2008/03/22/received PY - 2008/07/26/revised PY - 2008/07/27/accepted PY - 2008/11/26/pubmed PY - 2009/3/7/medline PY - 2008/11/26/entrez SP - 8 EP - 15 JF - Food microbiology JO - Food Microbiol VL - 26 IS - 1 N2 - Escherichia coli has been identified as the causative agent in numerous foodborne illness outbreaks associated with the consumption of fresh apple cider. Apple cider has a pH which is normally below 4.0 and would not be considered a medium capable of supporting the growth of foodborne pathogens. The association of unpasteurized apple cider with foodborne illness due to E. coli O157:H7 has however, led to increased interest in potential alternative methods to produce pathogen free cider. Apple cider was prepared from eight different apple cultivars, inoculated with approximately 10(6)-10(7) CFU of three strains of E. coli O157:H7 per ml (933, ATCC 43889, and ATCC 43895) and tested to determine the effectiveness of sulfur dioxide (SO(2)) and dimethyl dicarbonate (DMDC). Bacterial populations for treated and untreated samples were then enumerated by using non-selective media. Eight different ciders were treated with DMDC (125 and 250 ppm) and SO(2) (25, 50, 75, 100 ppm). Greater than a 5-log reduction was achieved at room temperature with 250 ppm of DMDC and 50 ppm of SO(2) after the incubation time of 6h and 24h, respectively. Addition of DMDC and/or SO(2) may offer an inexpensive alternative to thermal pasteurization for the production of safe apple cider for small apple cider producers. SN - 1095-9998 UR - https://www.unboundmedicine.com/medline/citation/19028298/Inactivation_of_different_strains_of_Escherichia_coli_O157:H7_in_various_apple_ciders_treated_with_dimethyl_dicarbonate__DMDC__and_sulfur_dioxide__SO2__as_an_alternative_method_ DB - PRIME DP - Unbound Medicine ER -