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Escherichia coli O157:H7 survival, biofilm formation and acid tolerance under simulated slaughter plant moist and dry conditions.
Food Microbiol. 2009 Feb; 26(1):112-9.FM

Abstract

Microorganisms persisting in slaughter plant environments may develop acid resistance and be translocated to other environmental surfaces or products. The objective of this study was to evaluate the potential of Escherichia coli O157:H7 to form biofilms and maintain acid resistance, under different culture habituation scenarios, on stainless steel coupons (2 x 5 x 0.08 cm), in the presence of beef carcass decontamination runoff fluids (washings). Coupons were stored in test tubes with unsterilized water washings (WW; pH 6.94) or lactic acid washings (LAW; pH 4.98), which were inoculated with E. coli O157:H7 (10(3)-10(4)CFU/ml) and incubated at 15 (24 or 48 h) or 35 degrees C (7 or 24 h), simulating different habituation scenarios on sites of a slaughter plant, including sanitation and overnight drying, during consecutive operational shifts. Acid resistance (AR) of planktonic and detached E. coli O157:H7 cells was assessed in tryptic soy broth adjusted to pH 3.5 with lactic acid. The highest pre-drying attachment and AR of E. coli O157:H7 were observed after 24h at 35 degrees C and 48 h at 15 degrees C. Drying reduced (P<0.05) recovery of attached E. coli O157:H7 cells; however, exposure of dried coupons to uninoculated washings allowed recovery of attached E. coli O157:H7, which restored AR, especially under conditions that favored post-drying growth. Exposure of attached cells to 50 ppm PAA for 45 s before drying, as well as habituation in LAW, reduced the recovery and AR of E. coli O157:H7. Therefore, incomplete removal of biofilms may result in cells of increased AR, especially in sites within a slaughter plant, in which liquid meat wastes may remain for long periods of time.

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Authors+Show Affiliations

Skandamis PN
Center for Meat Safety & Quality and Food Safety Cluster, Department of Animal Sciences, Colorado State University, Fort Collins, CO 80523-1171, USA.
Stopforth JD
No affiliation info available
Ashton LV
No affiliation info available
Geornaras I
No affiliation info available
Kendall PA
No affiliation info available
Sofos JN
No affiliation info available

MeSH

AbattoirsAdaptation, PhysiologicalAnimalsBiofilmsCattleColony Count, MicrobialDisinfectionEquipment ContaminationEscherichia coli O157Food ContaminationFood HandlingHydrogen-Ion ConcentrationStainless SteelTemperatureTime FactorsWater Microbiology

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19028314

Citation

Skandamis, Panagiotis N., et al. "Escherichia Coli O157:H7 Survival, Biofilm Formation and Acid Tolerance Under Simulated Slaughter Plant Moist and Dry Conditions." Food Microbiology, vol. 26, no. 1, 2009, pp. 112-9.
Skandamis PN, Stopforth JD, Ashton LV, et al. Escherichia coli O157:H7 survival, biofilm formation and acid tolerance under simulated slaughter plant moist and dry conditions. Food Microbiol. 2009;26(1):112-9.
Skandamis, P. N., Stopforth, J. D., Ashton, L. V., Geornaras, I., Kendall, P. A., & Sofos, J. N. (2009). Escherichia coli O157:H7 survival, biofilm formation and acid tolerance under simulated slaughter plant moist and dry conditions. Food Microbiology, 26(1), 112-9. https://doi.org/10.1016/j.fm.2008.09.001
Skandamis PN, et al. Escherichia Coli O157:H7 Survival, Biofilm Formation and Acid Tolerance Under Simulated Slaughter Plant Moist and Dry Conditions. Food Microbiol. 2009;26(1):112-9. PubMed PMID: 19028314.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Escherichia coli O157:H7 survival, biofilm formation and acid tolerance under simulated slaughter plant moist and dry conditions. AU - Skandamis,Panagiotis N, AU - Stopforth,Jarret D, AU - Ashton,Laura V, AU - Geornaras,Ifigenia, AU - Kendall,Patricia A, AU - Sofos,John N, Y1 - 2008/09/19/ PY - 2008/02/07/received PY - 2008/09/06/revised PY - 2008/09/07/accepted PY - 2008/11/26/pubmed PY - 2009/3/7/medline PY - 2008/11/26/entrez SP - 112 EP - 9 JF - Food microbiology JO - Food Microbiol VL - 26 IS - 1 N2 - Microorganisms persisting in slaughter plant environments may develop acid resistance and be translocated to other environmental surfaces or products. The objective of this study was to evaluate the potential of Escherichia coli O157:H7 to form biofilms and maintain acid resistance, under different culture habituation scenarios, on stainless steel coupons (2 x 5 x 0.08 cm), in the presence of beef carcass decontamination runoff fluids (washings). Coupons were stored in test tubes with unsterilized water washings (WW; pH 6.94) or lactic acid washings (LAW; pH 4.98), which were inoculated with E. coli O157:H7 (10(3)-10(4)CFU/ml) and incubated at 15 (24 or 48 h) or 35 degrees C (7 or 24 h), simulating different habituation scenarios on sites of a slaughter plant, including sanitation and overnight drying, during consecutive operational shifts. Acid resistance (AR) of planktonic and detached E. coli O157:H7 cells was assessed in tryptic soy broth adjusted to pH 3.5 with lactic acid. The highest pre-drying attachment and AR of E. coli O157:H7 were observed after 24h at 35 degrees C and 48 h at 15 degrees C. Drying reduced (P<0.05) recovery of attached E. coli O157:H7 cells; however, exposure of dried coupons to uninoculated washings allowed recovery of attached E. coli O157:H7, which restored AR, especially under conditions that favored post-drying growth. Exposure of attached cells to 50 ppm PAA for 45 s before drying, as well as habituation in LAW, reduced the recovery and AR of E. coli O157:H7. Therefore, incomplete removal of biofilms may result in cells of increased AR, especially in sites within a slaughter plant, in which liquid meat wastes may remain for long periods of time. SN - 1095-9998 UR - https://www.unboundmedicine.com/medline/citation/19028314/Escherichia_coli_O157:H7_survival_biofilm_formation_and_acid_tolerance_under_simulated_slaughter_plant_moist_and_dry_conditions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0740-0020(08)00176-7 DB - PRIME DP - Unbound Medicine ER -