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Attenuation of oxidative neuronal cell death by coffee phenolic phytochemicals.
Mutat Res. 2009 Feb 10; 661(1-2):18-24.MR

Abstract

Neurodegenerative disorders such as Alzheimer's disease (AD) are strongly associated with oxidative stress, which is induced by reactive oxygen species (ROS) including hydrogen peroxide (H(2)O(2)). Recent studies suggest that moderate coffee consumption may reduce the risk of neurodegenerative diseases such as AD, but the molecular mechanisms underlying this effect remain to be clarified. In this study, we investigated the protective effects of chlorogenic acid (5-O-caffeoylquinic acid; CGA), a major phenolic phytochemical found in instant decaffeinated coffee (IDC), and IDC against oxidative PC12 neuronal cell death. IDC (1 and 5 microg/ml) or CGA (1 and 5 microM) attenuated H(2)O(2)-induced PC12 cell death. H(2)O(2)-induced nuclear condensation and DNA fragmentation were strongly inhibited by pretreatment with IDC or CGA. Pretreatment with IDC or CGA also inhibited the H(2)O(2)-induced cleavage of poly(ADP-ribose) polymerase (PARP), and downregulation of Bcl-X(L) and caspase-3. The accumulation of intracellular ROS in H(2)O(2)-treated PC12 cells was dose-dependently diminished by IDC or CGA. The activation of c-Jun N-terminal protein kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) by H(2)O(2) in PC12 cells was also inhibited by IDC or CGA. Collectively, these results indicate that IDC and CGA protect PC12 cells from H(2)O(2)-induced apoptosis by blocking the accumulation of intracellular ROS and the activation of MAPKs.

Authors+Show Affiliations

Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19028509

Citation

Cho, Eun Sun, et al. "Attenuation of Oxidative Neuronal Cell Death By Coffee Phenolic Phytochemicals." Mutation Research, vol. 661, no. 1-2, 2009, pp. 18-24.
Cho ES, Jang YJ, Hwang MK, et al. Attenuation of oxidative neuronal cell death by coffee phenolic phytochemicals. Mutat Res. 2009;661(1-2):18-24.
Cho, E. S., Jang, Y. J., Hwang, M. K., Kang, N. J., Lee, K. W., & Lee, H. J. (2009). Attenuation of oxidative neuronal cell death by coffee phenolic phytochemicals. Mutation Research, 661(1-2), 18-24. https://doi.org/10.1016/j.mrfmmm.2008.10.021
Cho ES, et al. Attenuation of Oxidative Neuronal Cell Death By Coffee Phenolic Phytochemicals. Mutat Res. 2009 Feb 10;661(1-2):18-24. PubMed PMID: 19028509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Attenuation of oxidative neuronal cell death by coffee phenolic phytochemicals. AU - Cho,Eun Sun, AU - Jang,Young Jin, AU - Hwang,Mun Kyung, AU - Kang,Nam Joo, AU - Lee,Ki Won, AU - Lee,Hyong Joo, Y1 - 2008/11/05/ PY - 2008/05/02/received PY - 2008/10/22/revised PY - 2008/10/24/accepted PY - 2008/11/26/entrez PY - 2008/11/26/pubmed PY - 2009/4/17/medline SP - 18 EP - 24 JF - Mutation research JO - Mutat Res VL - 661 IS - 1-2 N2 - Neurodegenerative disorders such as Alzheimer's disease (AD) are strongly associated with oxidative stress, which is induced by reactive oxygen species (ROS) including hydrogen peroxide (H(2)O(2)). Recent studies suggest that moderate coffee consumption may reduce the risk of neurodegenerative diseases such as AD, but the molecular mechanisms underlying this effect remain to be clarified. In this study, we investigated the protective effects of chlorogenic acid (5-O-caffeoylquinic acid; CGA), a major phenolic phytochemical found in instant decaffeinated coffee (IDC), and IDC against oxidative PC12 neuronal cell death. IDC (1 and 5 microg/ml) or CGA (1 and 5 microM) attenuated H(2)O(2)-induced PC12 cell death. H(2)O(2)-induced nuclear condensation and DNA fragmentation were strongly inhibited by pretreatment with IDC or CGA. Pretreatment with IDC or CGA also inhibited the H(2)O(2)-induced cleavage of poly(ADP-ribose) polymerase (PARP), and downregulation of Bcl-X(L) and caspase-3. The accumulation of intracellular ROS in H(2)O(2)-treated PC12 cells was dose-dependently diminished by IDC or CGA. The activation of c-Jun N-terminal protein kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) by H(2)O(2) in PC12 cells was also inhibited by IDC or CGA. Collectively, these results indicate that IDC and CGA protect PC12 cells from H(2)O(2)-induced apoptosis by blocking the accumulation of intracellular ROS and the activation of MAPKs. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/19028509/Attenuation_of_oxidative_neuronal_cell_death_by_coffee_phenolic_phytochemicals_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0027-5107(08)00259-5 DB - PRIME DP - Unbound Medicine ER -