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Carbapenem-hydrolysing beta-lactamase KPC-2 in Klebsiella pneumoniae isolated in Rio de Janeiro, Brazil.
J Antimicrob Chemother. 2009 Feb; 63(2):265-8.JA

Abstract

OBJECTIVES

The aim of this study was to characterize the KPC-type carbapenem-hydrolysing beta-lactamase, extended-spectrum beta-lactamases (ESBLs) and class 1 integrons among nosocomial Klebsiella pneumoniae isolated in Rio de Janeiro, Brazil.

METHODS

MICs were determined and isolates were screened for ESBLs, metallo-beta-lactamases (MBLs) and class A carbapenemase-producing phenotypes. The main beta-lactamases resistance genes (bla(TEM), bla(SHV), bla(CTX-M), bla(KPC), bla(IMP) and bla(VIM)) and class 1 integrons were detected by PCR followed by DNA sequencing. The genetic relatedness of isolates was determined by PFGE.

RESULTS

All K. pneumoniae isolates were positive for ESBL and class A carbapenemase production and negative for MBL production. All isolates were resistant to all beta-lactam antibiotics, ciprofloxacin and gentamicin, being susceptible only to tigecycline and polymyxin B. The bla(KPC-2), bla(CTX-M-1), bla(CTX-M-2), bla(CTX-M-8) and bla(SHV-11) genes were detected. PFGE analysis revealed two clonal types among KPC-producing isolates, both identified in the same hospital.

CONCLUSIONS

Our findings should alert medical authorities to implement stringent methods for the detection and spread control of emerging KPC-2 carbapenemases in the hospital setting in Brazil.

Authors+Show Affiliations

Oswaldo Cruz Institute, Avenida Brasil 4365, 21040-360 Rio de Janeiro, Brazil. peirano@ioc.fiocruz.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19028717

Citation

Peirano, Gisele, et al. "Carbapenem-hydrolysing Beta-lactamase KPC-2 in Klebsiella Pneumoniae Isolated in Rio De Janeiro, Brazil." The Journal of Antimicrobial Chemotherapy, vol. 63, no. 2, 2009, pp. 265-8.
Peirano G, Seki LM, Val Passos VL, et al. Carbapenem-hydrolysing beta-lactamase KPC-2 in Klebsiella pneumoniae isolated in Rio de Janeiro, Brazil. J Antimicrob Chemother. 2009;63(2):265-8.
Peirano, G., Seki, L. M., Val Passos, V. L., Pinto, M. C., Guerra, L. R., & Asensi, M. D. (2009). Carbapenem-hydrolysing beta-lactamase KPC-2 in Klebsiella pneumoniae isolated in Rio de Janeiro, Brazil. The Journal of Antimicrobial Chemotherapy, 63(2), 265-8. https://doi.org/10.1093/jac/dkn484
Peirano G, et al. Carbapenem-hydrolysing Beta-lactamase KPC-2 in Klebsiella Pneumoniae Isolated in Rio De Janeiro, Brazil. J Antimicrob Chemother. 2009;63(2):265-8. PubMed PMID: 19028717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carbapenem-hydrolysing beta-lactamase KPC-2 in Klebsiella pneumoniae isolated in Rio de Janeiro, Brazil. AU - Peirano,Gisele, AU - Seki,Liliane M, AU - Val Passos,Vera Lúcia, AU - Pinto,Maria Cristina F G, AU - Guerra,Lília R, AU - Asensi,Marise D, Y1 - 2008/11/20/ PY - 2008/11/26/pubmed PY - 2009/2/17/medline PY - 2008/11/26/entrez SP - 265 EP - 8 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 63 IS - 2 N2 - OBJECTIVES: The aim of this study was to characterize the KPC-type carbapenem-hydrolysing beta-lactamase, extended-spectrum beta-lactamases (ESBLs) and class 1 integrons among nosocomial Klebsiella pneumoniae isolated in Rio de Janeiro, Brazil. METHODS: MICs were determined and isolates were screened for ESBLs, metallo-beta-lactamases (MBLs) and class A carbapenemase-producing phenotypes. The main beta-lactamases resistance genes (bla(TEM), bla(SHV), bla(CTX-M), bla(KPC), bla(IMP) and bla(VIM)) and class 1 integrons were detected by PCR followed by DNA sequencing. The genetic relatedness of isolates was determined by PFGE. RESULTS: All K. pneumoniae isolates were positive for ESBL and class A carbapenemase production and negative for MBL production. All isolates were resistant to all beta-lactam antibiotics, ciprofloxacin and gentamicin, being susceptible only to tigecycline and polymyxin B. The bla(KPC-2), bla(CTX-M-1), bla(CTX-M-2), bla(CTX-M-8) and bla(SHV-11) genes were detected. PFGE analysis revealed two clonal types among KPC-producing isolates, both identified in the same hospital. CONCLUSIONS: Our findings should alert medical authorities to implement stringent methods for the detection and spread control of emerging KPC-2 carbapenemases in the hospital setting in Brazil. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/19028717/Carbapenem_hydrolysing_beta_lactamase_KPC_2_in_Klebsiella_pneumoniae_isolated_in_Rio_de_Janeiro_Brazil_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkn484 DB - PRIME DP - Unbound Medicine ER -