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A study on the ALAD gene polymorphisms associated with lead exposure.
Toxicol Ind Health 2008; 24(7):501-6TI

Abstract

Delta -aminolevulinic acid dehydratase (ALAD) plays an important role in lead poisoning, and polymorphisms in this gene may affect the response of individuals to lead toxicity symptoms. This study was carried out to examine the effects of ALAD gene polymorphism (G177C) on blood lead levels (BLL) and hematological parameters. In all, 113 battery manufacturing unit workers and 102 occupationally unexposed controls from Hyderabad, A.P, India formed the study group. Genotypes for the ALAD G177C polymorphism were determined by Polymerase chain reaction and restriction fragment length digestion. BLL were determined by anode stripping voltammetry using ESA Model 3010B Lead analyzer. Complete blood picture was analyzed using ADVIA cell counter for each sample. The frequencies for ALAD1 and ALAD2 alleles were 0.98 and 0.01, respectively. ALAD 1-2 and ALAD 2-2 genotypes together were considered as a single group and compared with the ALAD 1-1 genotype group. BLL did not differ significantly among ALAD1-1, 1-2, and 2-2 genotypes; however, subjects from the ALAD 1-2/2-2 genotype group showed higher BLL concentrations of 80.51 microg/dL when compared with subjects from the ALAD 1-1 genotype group (50.4 microg/dL). Approximately 29.2% volunteers (nA =A 33) from the occupationally exposed group had hemoglobin levels below 10.0 g/dL. There was no significant difference in total white cell count and platelet count between occupational and non-exposed lead-exposed groups. BLL of occupationally exposed individuals were significantly high compared with the unexposed group. ALAD G177C polymorphism along with BLL and assessment of hematological parameters may play an important role in evaluation and better understanding of the consequences of lead exposure.

Authors+Show Affiliations

Department of Genetics, Bhagwan Mahavir Medical Research Centre, Hyderabad, AP, India.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19028776

Citation

Shaik, A P., and K Jamil. "A Study On the ALAD Gene Polymorphisms Associated With Lead Exposure." Toxicology and Industrial Health, vol. 24, no. 7, 2008, pp. 501-6.
Shaik AP, Jamil K. A study on the ALAD gene polymorphisms associated with lead exposure. Toxicol Ind Health. 2008;24(7):501-6.
Shaik, A. P., & Jamil, K. (2008). A study on the ALAD gene polymorphisms associated with lead exposure. Toxicology and Industrial Health, 24(7), pp. 501-6. doi:10.1177/0748233708095770.
Shaik AP, Jamil K. A Study On the ALAD Gene Polymorphisms Associated With Lead Exposure. Toxicol Ind Health. 2008;24(7):501-6. PubMed PMID: 19028776.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A study on the ALAD gene polymorphisms associated with lead exposure. AU - Shaik,A P, AU - Jamil,K, PY - 2008/11/26/pubmed PY - 2009/3/11/medline PY - 2008/11/26/entrez SP - 501 EP - 6 JF - Toxicology and industrial health JO - Toxicol Ind Health VL - 24 IS - 7 N2 - Delta -aminolevulinic acid dehydratase (ALAD) plays an important role in lead poisoning, and polymorphisms in this gene may affect the response of individuals to lead toxicity symptoms. This study was carried out to examine the effects of ALAD gene polymorphism (G177C) on blood lead levels (BLL) and hematological parameters. In all, 113 battery manufacturing unit workers and 102 occupationally unexposed controls from Hyderabad, A.P, India formed the study group. Genotypes for the ALAD G177C polymorphism were determined by Polymerase chain reaction and restriction fragment length digestion. BLL were determined by anode stripping voltammetry using ESA Model 3010B Lead analyzer. Complete blood picture was analyzed using ADVIA cell counter for each sample. The frequencies for ALAD1 and ALAD2 alleles were 0.98 and 0.01, respectively. ALAD 1-2 and ALAD 2-2 genotypes together were considered as a single group and compared with the ALAD 1-1 genotype group. BLL did not differ significantly among ALAD1-1, 1-2, and 2-2 genotypes; however, subjects from the ALAD 1-2/2-2 genotype group showed higher BLL concentrations of 80.51 microg/dL when compared with subjects from the ALAD 1-1 genotype group (50.4 microg/dL). Approximately 29.2% volunteers (nA =A 33) from the occupationally exposed group had hemoglobin levels below 10.0 g/dL. There was no significant difference in total white cell count and platelet count between occupational and non-exposed lead-exposed groups. BLL of occupationally exposed individuals were significantly high compared with the unexposed group. ALAD G177C polymorphism along with BLL and assessment of hematological parameters may play an important role in evaluation and better understanding of the consequences of lead exposure. SN - 0748-2337 UR - https://www.unboundmedicine.com/medline/citation/19028776/A_study_on_the_ALAD_gene_polymorphisms_associated_with_lead_exposure_ L2 - http://journals.sagepub.com/doi/full/10.1177/0748233708095770?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -