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Differential localizations of the transient receptor potential channels TRPV4 and TRPV1 in the mouse urinary bladder.
J Histochem Cytochem. 2009 Mar; 57(3):277-87.JH

Abstract

We studied the localization and physiological functions of the transient receptor potential (TRP) channels TRPV1 (TRP vanilloid 1) and TRPV4 (TRP vanilloid 4) in the mouse bladder, because both channels are thought to be mechanosensors for bladder distention. RT-PCR specifically amplified TRPV4 transcripts from the urothelial cells, whereas TRPV1 transcripts were barely detectable. ISH experiments showed that TRPV4 transcripts were abundantly expressed in the urothelium, whereas TRPV1 transcripts were not detectable in the urothelial cells. Immunoblotting and IHC studies showed that TRPV4 proteins were mainly localized at the basal plasma membrane domains of the basal urothelial cells. In contrast, TRPV1-immunoreactivities were found not in the urothelial cells but in the nerve fibers that innervate the urinary bladder. In Ca(2+)-imaging experiments, 4alpha-phorbol 12,13-didecanoate, a TRPV4 agonist, and hypotonic stimuli induced significant increases in intracellular calcium ion concentration ([Ca(2+)](i)) in isolated urothelial cells, whereas capsaicin, a TRPV1 agonist, showed no marked effect on the cells. These findings raise the possibility that, in mouse urothelial cells, TRPV4 may contribute to the detection of increases in intravesical pressure related to the micturition reflex.

Authors+Show Affiliations

Department of Neurobiology and Anatomy, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19029406

Citation

Yamada, Takahiro, et al. "Differential Localizations of the Transient Receptor Potential Channels TRPV4 and TRPV1 in the Mouse Urinary Bladder." The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society, vol. 57, no. 3, 2009, pp. 277-87.
Yamada T, Ugawa S, Ueda T, et al. Differential localizations of the transient receptor potential channels TRPV4 and TRPV1 in the mouse urinary bladder. J Histochem Cytochem. 2009;57(3):277-87.
Yamada, T., Ugawa, S., Ueda, T., Ishida, Y., Kajita, K., & Shimada, S. (2009). Differential localizations of the transient receptor potential channels TRPV4 and TRPV1 in the mouse urinary bladder. The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society, 57(3), 277-87. https://doi.org/10.1369/jhc.2008.951962
Yamada T, et al. Differential Localizations of the Transient Receptor Potential Channels TRPV4 and TRPV1 in the Mouse Urinary Bladder. J Histochem Cytochem. 2009;57(3):277-87. PubMed PMID: 19029406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential localizations of the transient receptor potential channels TRPV4 and TRPV1 in the mouse urinary bladder. AU - Yamada,Takahiro, AU - Ugawa,Shinya, AU - Ueda,Takashi, AU - Ishida,Yusuke, AU - Kajita,Kenji, AU - Shimada,Shoichi, Y1 - 2008/11/24/ PY - 2008/11/26/pubmed PY - 2009/3/24/medline PY - 2008/11/26/entrez SP - 277 EP - 87 JF - The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society JO - J Histochem Cytochem VL - 57 IS - 3 N2 - We studied the localization and physiological functions of the transient receptor potential (TRP) channels TRPV1 (TRP vanilloid 1) and TRPV4 (TRP vanilloid 4) in the mouse bladder, because both channels are thought to be mechanosensors for bladder distention. RT-PCR specifically amplified TRPV4 transcripts from the urothelial cells, whereas TRPV1 transcripts were barely detectable. ISH experiments showed that TRPV4 transcripts were abundantly expressed in the urothelium, whereas TRPV1 transcripts were not detectable in the urothelial cells. Immunoblotting and IHC studies showed that TRPV4 proteins were mainly localized at the basal plasma membrane domains of the basal urothelial cells. In contrast, TRPV1-immunoreactivities were found not in the urothelial cells but in the nerve fibers that innervate the urinary bladder. In Ca(2+)-imaging experiments, 4alpha-phorbol 12,13-didecanoate, a TRPV4 agonist, and hypotonic stimuli induced significant increases in intracellular calcium ion concentration ([Ca(2+)](i)) in isolated urothelial cells, whereas capsaicin, a TRPV1 agonist, showed no marked effect on the cells. These findings raise the possibility that, in mouse urothelial cells, TRPV4 may contribute to the detection of increases in intravesical pressure related to the micturition reflex. SN - 0022-1554 UR - https://www.unboundmedicine.com/medline/citation/19029406/Differential_localizations_of_the_transient_receptor_potential_channels_TRPV4_and_TRPV1_in_the_mouse_urinary_bladder_ L2 - https://journals.sagepub.com/doi/10.1369/jhc.2008.951962?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -