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ABM/P-15 modulates proliferation and mRNA synthesis of growth factors of periodontal ligament cells.
Acta Odontol Scand. 2009; 67(2):65-73.AO

Abstract

OBJECTIVE

Periodontal regeneration is histologically defined as regeneration of the tooth supporting structures, including alveolar bone, periodontal ligament, and cementum. Cells in the remaining periodontal tissues need optimal conditions if they are to perform their functions in the regeneration process. The present study is an investigation of the molecular effects of ABM/P-15 on human periodontal ligament cells (PDL) in vitro.

MATERIAL AND METHODS

PDL cells obtained from healthy subjects were used for in vitro experiments. Cell proliferation, morphology, and mineralization using Von kossa staining were evaluated. mRNA expressions for transforming growth factor-beta (TGF-beta), insulin-like growth factor-I (IGF-I), basic fibroblast growth factor (b-FGF), vascular endothelial growth factor (VEGF), bone morphogenic protein-2 (BMP-2), platelet-derived growth factor (PDGF), and type 1 collagen (COL1) were assessed on days 3 and 7 using RT-PCR.

RESULTS

ABM/P-15 enhanced proliferation of cultured PDL cells. It increased the mRNA expression of TGF-beta and BMP-2 in cultured PDL cells on days 3 and 7. IGF-I and b-FGF mRNA expressions showed a slight decrease, while PDGF expression was observed to have increased on day 3. VEGF and COL1 mRNA expressions were found not to be different on days 3 and 7. No differences were observed in the mineralization properties of cultured PDL cells treated with or without ABM/P-15.

CONCLUSIONS

Based on the results of this in vitro study, it may be concluded that ABM/P-15 enhanced the regenerative capacity of PDL by regulating specific gene expressions of cells during early wound healing.

Authors+Show Affiliations

Department of Periodontology, College of Dentistry, Ohio State University, Colombus, OH, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19031159

Citation

Emecen, Pinar, et al. "ABM/P-15 Modulates Proliferation and mRNA Synthesis of Growth Factors of Periodontal Ligament Cells." Acta Odontologica Scandinavica, vol. 67, no. 2, 2009, pp. 65-73.
Emecen P, Akman AC, Hakki SS, et al. ABM/P-15 modulates proliferation and mRNA synthesis of growth factors of periodontal ligament cells. Acta Odontol Scand. 2009;67(2):65-73.
Emecen, P., Akman, A. C., Hakki, S. S., Hakki, E. E., Demiralp, B., Tözüm, T. F., & Nohutcu, R. M. (2009). ABM/P-15 modulates proliferation and mRNA synthesis of growth factors of periodontal ligament cells. Acta Odontologica Scandinavica, 67(2), 65-73. https://doi.org/10.1080/00016350802555525
Emecen P, et al. ABM/P-15 Modulates Proliferation and mRNA Synthesis of Growth Factors of Periodontal Ligament Cells. Acta Odontol Scand. 2009;67(2):65-73. PubMed PMID: 19031159.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ABM/P-15 modulates proliferation and mRNA synthesis of growth factors of periodontal ligament cells. AU - Emecen,Pinar, AU - Akman,Abdullah Cevdet, AU - Hakki,Sema Sezgin, AU - Hakki,Erdogan Esref, AU - Demiralp,Burak, AU - Tözüm,Tolga Fikret, AU - Nohutcu,Rahime Meral, PY - 2008/11/26/pubmed PY - 2009/5/20/medline PY - 2008/11/26/entrez SP - 65 EP - 73 JF - Acta odontologica Scandinavica JO - Acta Odontol. Scand. VL - 67 IS - 2 N2 - OBJECTIVE: Periodontal regeneration is histologically defined as regeneration of the tooth supporting structures, including alveolar bone, periodontal ligament, and cementum. Cells in the remaining periodontal tissues need optimal conditions if they are to perform their functions in the regeneration process. The present study is an investigation of the molecular effects of ABM/P-15 on human periodontal ligament cells (PDL) in vitro. MATERIAL AND METHODS: PDL cells obtained from healthy subjects were used for in vitro experiments. Cell proliferation, morphology, and mineralization using Von kossa staining were evaluated. mRNA expressions for transforming growth factor-beta (TGF-beta), insulin-like growth factor-I (IGF-I), basic fibroblast growth factor (b-FGF), vascular endothelial growth factor (VEGF), bone morphogenic protein-2 (BMP-2), platelet-derived growth factor (PDGF), and type 1 collagen (COL1) were assessed on days 3 and 7 using RT-PCR. RESULTS: ABM/P-15 enhanced proliferation of cultured PDL cells. It increased the mRNA expression of TGF-beta and BMP-2 in cultured PDL cells on days 3 and 7. IGF-I and b-FGF mRNA expressions showed a slight decrease, while PDGF expression was observed to have increased on day 3. VEGF and COL1 mRNA expressions were found not to be different on days 3 and 7. No differences were observed in the mineralization properties of cultured PDL cells treated with or without ABM/P-15. CONCLUSIONS: Based on the results of this in vitro study, it may be concluded that ABM/P-15 enhanced the regenerative capacity of PDL by regulating specific gene expressions of cells during early wound healing. SN - 1502-3850 UR - https://www.unboundmedicine.com/medline/citation/19031159/ABM/P_15_modulates_proliferation_and_mRNA_synthesis_of_growth_factors_of_periodontal_ligament_cells_ L2 - http://www.tandfonline.com/doi/full/10.1080/00016350802555525 DB - PRIME DP - Unbound Medicine ER -