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Witch hazel (Hamamelis virginiana) fractions and the importance of gallate moieties--electron transfer capacities in their antitumoral properties.
J Agric Food Chem. 2008 Dec 24; 56(24):11675-82.JA

Abstract

Witch hazel (Hamamelis virginia) extracts are used in traditional medicine. They are particularly rich in gallate esters included in proanthocyanidins, hydrolyzable tannins (galloylated sugars), and methyl gallate. This study examines the response of human colon cancer cells to treatment with fractions obtained from a witch hazel polyphenolic extract. The results are compared with those obtained previously with homologous fractions from grape (less galloylated) and pine (nongalloylated). Witch hazel fractions were the most efficient in inhibiting cell proliferation in HT29 and HCT116 human colon cancer cell lines, which clearly shows that the more galloylated the fractions, the more effective they were at inhibiting proliferation of colon cancer cells. Witch hazel fractions were, in addition, more potent in arresting the cell cycle at the S phase and inducing apoptosis; they also induced a significant percentage of necrosis. Interestingly, the apoptosis and cell cycle arrest effects induced were proportional to their galloylation. Moreover, witch hazel fractions with a high degree of galloylation were also the most effective as scavengers of both hydroxyl and superoxide radicals and in protecting against DNA damage triggered by the hydroxyl radical system. These findings provide a better understanding of the structure-bioactivity relationships of polyphenolics, which should be of assistance in choosing an appropriate source and preparing a rational design for formulations of plant polyphenols in nutritional supplements.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Lizárraga D
Department of Biochemistry and Molecular Biology, unit associated with CSIC, Faculty of Biology, University of Barcelona, Biomedicine Institute of the University of Barcelona, Diagonal 645, E-08028 Barcelona, Spain.
Touriño S
No affiliation info available
Reyes-Zurita FJ
No affiliation info available
de Kok TM
No affiliation info available
van Delft JH
No affiliation info available
Maas LM
No affiliation info available
Briedé JJ
No affiliation info available
Centelles JJ
No affiliation info available
Torres JL
No affiliation info available
Cascante M
No affiliation info available

MeSH

Antineoplastic AgentsApoptosisCell CycleCell Line, TumorCell ProliferationColonic NeoplasmsFlavonoidsGallic AcidHamamelisHumansPhenolsPlant ExtractsPolyphenolsStructure-Activity Relationship

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19035659

Citation

Lizárraga, Daneida, et al. "Witch Hazel (Hamamelis Virginiana) Fractions and the Importance of Gallate Moieties--electron Transfer Capacities in Their Antitumoral Properties." Journal of Agricultural and Food Chemistry, vol. 56, no. 24, 2008, pp. 11675-82.
Lizárraga D, Touriño S, Reyes-Zurita FJ, et al. Witch hazel (Hamamelis virginiana) fractions and the importance of gallate moieties--electron transfer capacities in their antitumoral properties. J Agric Food Chem. 2008;56(24):11675-82.
Lizárraga, D., Touriño, S., Reyes-Zurita, F. J., de Kok, T. M., van Delft, J. H., Maas, L. M., Briedé, J. J., Centelles, J. J., Torres, J. L., & Cascante, M. (2008). Witch hazel (Hamamelis virginiana) fractions and the importance of gallate moieties--electron transfer capacities in their antitumoral properties. Journal of Agricultural and Food Chemistry, 56(24), 11675-82. https://doi.org/10.1021/jf802345x
Lizárraga D, et al. Witch Hazel (Hamamelis Virginiana) Fractions and the Importance of Gallate Moieties--electron Transfer Capacities in Their Antitumoral Properties. J Agric Food Chem. 2008 Dec 24;56(24):11675-82. PubMed PMID: 19035659.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Witch hazel (Hamamelis virginiana) fractions and the importance of gallate moieties--electron transfer capacities in their antitumoral properties. AU - Lizárraga,Daneida, AU - Touriño,Sonia, AU - Reyes-Zurita,Fernando J, AU - de Kok,Theo M, AU - van Delft,Joost H, AU - Maas,Lou M, AU - Briedé,Jacco J, AU - Centelles,Josep J, AU - Torres,Josep L, AU - Cascante,Marta, PY - 2008/11/28/pubmed PY - 2009/1/27/medline PY - 2008/11/28/entrez SP - 11675 EP - 82 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 56 IS - 24 N2 - Witch hazel (Hamamelis virginia) extracts are used in traditional medicine. They are particularly rich in gallate esters included in proanthocyanidins, hydrolyzable tannins (galloylated sugars), and methyl gallate. This study examines the response of human colon cancer cells to treatment with fractions obtained from a witch hazel polyphenolic extract. The results are compared with those obtained previously with homologous fractions from grape (less galloylated) and pine (nongalloylated). Witch hazel fractions were the most efficient in inhibiting cell proliferation in HT29 and HCT116 human colon cancer cell lines, which clearly shows that the more galloylated the fractions, the more effective they were at inhibiting proliferation of colon cancer cells. Witch hazel fractions were, in addition, more potent in arresting the cell cycle at the S phase and inducing apoptosis; they also induced a significant percentage of necrosis. Interestingly, the apoptosis and cell cycle arrest effects induced were proportional to their galloylation. Moreover, witch hazel fractions with a high degree of galloylation were also the most effective as scavengers of both hydroxyl and superoxide radicals and in protecting against DNA damage triggered by the hydroxyl radical system. These findings provide a better understanding of the structure-bioactivity relationships of polyphenolics, which should be of assistance in choosing an appropriate source and preparing a rational design for formulations of plant polyphenols in nutritional supplements. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19035659/Witch_hazel__Hamamelis_virginiana__fractions_and_the_importance_of_gallate_moieties__electron_transfer_capacities_in_their_antitumoral_properties_ L2 - https://doi.org/10.1021/jf802345x DB - PRIME DP - Unbound Medicine ER -