Tags

Type your tag names separated by a space and hit enter

Determination of paclitaxel and other six taxoids in Taxus species by high-performance liquid chromatography-tandem mass spectrometry.
J Pharm Biomed Anal. 2009 Jan 15; 49(1):81-9.JP

Abstract

A method of high-performance liquid chromatography-tandem mass spectrometry (LC-MS-MS) has been developed for the trace analysis of paclitaxel and other six taxoids in three Taxus species including Taxus cuspidata, Taxus media and Taxus chinensis var. mairiei. Seven taxoids were separated using a gradient mode on an Eclipse XDB-C18 column (4.6x150 mm; i.d., 5 microm) at 20 degrees C. The compound separations were detected by an API 3000 mass spectrometer equipped with a TurboIonSpray interface. The compounds were detected using electrospray ionization (ESI) in positive-ion mode and quantified by multiple-reaction monitoring (MRM) mode using the transition mass of m/z 567.4-->445.4, 609.3-->549.5, 944.9-->286.4, 812.6-->286.1, 832.8-->264.1, 854.4-->286.1, and 812.6-->286.1 for 10-DAB III, baccatin III, 7-xyl-10-DAT, 10-DAT, cephalomannine, paclitaxel, and 7-epi-10-DAT, respectively. The ranges of limit of quantitation (LOQ) for taxoids were 32, 14, 26, 14, 20, 32, and 26 ng/mL for 10-DAB III, baccatin III, 7-xyl-10-DAT, 10-DAT, cephalomannine, paclitaxel, and 7-epi-10-DAT, respectively. Linearity was confirmed over the whole calibration range (0.07-45, 0.058-37.5, 0.058-37.5, 0.056-36.3, 0.053-33.8, 0.057-37.5, and 0.06-38.8 microg/mL for 10-DAB III, baccatin III, 7-xyl-10-DAT, 10-DAT, cephalomannine, paclitaxel, and 7-epi-10-DAT, respectively) with coefficients higher than 0.9903. The inter- and intra-day precision of taxoids ranged from 2.86% to 6.31% for retention time and ranged from 3.91% to 7.33% for peak area. The recovery rates of this method were higher than 94.32% for 10-DAB III, 94.68% for baccatin III, 93.65% for 7-xyl-10-DAT, 93.29% for 10-DAT, 92.91% for cephalomannine, 93.41% for paclitaxel, and 93.06% for 7-epi-10-DAT, respectively.

Authors+Show Affiliations

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19036549

Citation

Li, Shuangming, et al. "Determination of Paclitaxel and Other Six Taxoids in Taxus Species By High-performance Liquid Chromatography-tandem Mass Spectrometry." Journal of Pharmaceutical and Biomedical Analysis, vol. 49, no. 1, 2009, pp. 81-9.
Li S, Fu Y, Zu Y, et al. Determination of paclitaxel and other six taxoids in Taxus species by high-performance liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal. 2009;49(1):81-9.
Li, S., Fu, Y., Zu, Y., Sun, R., Wang, Y., Zhang, L., Luo, H., Gu, C., & Efferth, T. (2009). Determination of paclitaxel and other six taxoids in Taxus species by high-performance liquid chromatography-tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 49(1), 81-9. https://doi.org/10.1016/j.jpba.2008.10.006
Li S, et al. Determination of Paclitaxel and Other Six Taxoids in Taxus Species By High-performance Liquid Chromatography-tandem Mass Spectrometry. J Pharm Biomed Anal. 2009 Jan 15;49(1):81-9. PubMed PMID: 19036549.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of paclitaxel and other six taxoids in Taxus species by high-performance liquid chromatography-tandem mass spectrometry. AU - Li,Shuangming, AU - Fu,Yujie, AU - Zu,Yuangang, AU - Sun,Rui, AU - Wang,Ying, AU - Zhang,Lin, AU - Luo,Hao, AU - Gu,Chengbo, AU - Efferth,Thomas, Y1 - 2008/10/19/ PY - 2008/07/09/received PY - 2008/10/07/revised PY - 2008/10/08/accepted PY - 2008/11/28/pubmed PY - 2009/4/3/medline PY - 2008/11/28/entrez SP - 81 EP - 9 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 49 IS - 1 N2 - A method of high-performance liquid chromatography-tandem mass spectrometry (LC-MS-MS) has been developed for the trace analysis of paclitaxel and other six taxoids in three Taxus species including Taxus cuspidata, Taxus media and Taxus chinensis var. mairiei. Seven taxoids were separated using a gradient mode on an Eclipse XDB-C18 column (4.6x150 mm; i.d., 5 microm) at 20 degrees C. The compound separations were detected by an API 3000 mass spectrometer equipped with a TurboIonSpray interface. The compounds were detected using electrospray ionization (ESI) in positive-ion mode and quantified by multiple-reaction monitoring (MRM) mode using the transition mass of m/z 567.4-->445.4, 609.3-->549.5, 944.9-->286.4, 812.6-->286.1, 832.8-->264.1, 854.4-->286.1, and 812.6-->286.1 for 10-DAB III, baccatin III, 7-xyl-10-DAT, 10-DAT, cephalomannine, paclitaxel, and 7-epi-10-DAT, respectively. The ranges of limit of quantitation (LOQ) for taxoids were 32, 14, 26, 14, 20, 32, and 26 ng/mL for 10-DAB III, baccatin III, 7-xyl-10-DAT, 10-DAT, cephalomannine, paclitaxel, and 7-epi-10-DAT, respectively. Linearity was confirmed over the whole calibration range (0.07-45, 0.058-37.5, 0.058-37.5, 0.056-36.3, 0.053-33.8, 0.057-37.5, and 0.06-38.8 microg/mL for 10-DAB III, baccatin III, 7-xyl-10-DAT, 10-DAT, cephalomannine, paclitaxel, and 7-epi-10-DAT, respectively) with coefficients higher than 0.9903. The inter- and intra-day precision of taxoids ranged from 2.86% to 6.31% for retention time and ranged from 3.91% to 7.33% for peak area. The recovery rates of this method were higher than 94.32% for 10-DAB III, 94.68% for baccatin III, 93.65% for 7-xyl-10-DAT, 93.29% for 10-DAT, 92.91% for cephalomannine, 93.41% for paclitaxel, and 93.06% for 7-epi-10-DAT, respectively. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/19036549/Determination_of_paclitaxel_and_other_six_taxoids_in_Taxus_species_by_high_performance_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(08)00567-0 DB - PRIME DP - Unbound Medicine ER -