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Phytochemical and genetic analyses of ancient cannabis from Central Asia.

Abstract

The Yanghai Tombs near Turpan, Xinjiang-Uighur Autonomous Region, China have recently been excavated to reveal the 2700-year-old grave of a Caucasoid shaman whose accoutrements included a large cache of cannabis, superbly preserved by climatic and burial conditions. A multidisciplinary international team demonstrated through botanical examination, phytochemical investigation, and genetic deoxyribonucleic acid analysis by polymerase chain reaction that this material contained tetrahydrocannabinol, the psychoactive component of cannabis, its oxidative degradation product, cannabinol, other metabolites, and its synthetic enzyme, tetrahydrocannabinolic acid synthase, as well as a novel genetic variant with two single nucleotide polymorphisms. The cannabis was presumably employed by this culture as a medicinal or psychoactive agent, or an aid to divination. To our knowledge, these investigations provide the oldest documentation of cannabis as a pharmacologically active agent, and contribute to the medical and archaeological record of this pre-Silk Road culture.

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  • Authors+Show Affiliations

    ,

    Institute of Botany, Chinese Academy of Sciences. erusso@gwpharm.com

    , , , , , , , , , , , , , , , ,

    Source

    Journal of experimental botany 59:15 2008 pg 4171-82

    MeSH

    Amino Acid Sequence
    Base Sequence
    Cannabidiol
    Cannabis
    China
    Chromatography, High Pressure Liquid
    Mass Spectrometry
    Molecular Sequence Data
    Plant Proteins

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    19036842

    Citation

    TY - JOUR T1 - Phytochemical and genetic analyses of ancient cannabis from Central Asia. AU - Russo,Ethan B, AU - Jiang,Hong-En, AU - Li,Xiao, AU - Sutton,Alan, AU - Carboni,Andrea, AU - del Bianco,Francesca, AU - Mandolino,Giuseppe, AU - Potter,David J, AU - Zhao,You-Xing, AU - Bera,Subir, AU - Zhang,Yong-Bing, AU - Lü,En-Guo, AU - Ferguson,David K, AU - Hueber,Francis, AU - Zhao,Liang-Cheng, AU - Liu,Chang-Jiang, AU - Wang,Yu-Fei, AU - Li,Cheng-Sen, PY - 2008/11/28/pubmed PY - 2009/1/1/medline PY - 2008/11/28/entrez SP - 4171 EP - 82 JF - Journal of experimental botany JO - J. Exp. Bot. VL - 59 IS - 15 N2 - The Yanghai Tombs near Turpan, Xinjiang-Uighur Autonomous Region, China have recently been excavated to reveal the 2700-year-old grave of a Caucasoid shaman whose accoutrements included a large cache of cannabis, superbly preserved by climatic and burial conditions. A multidisciplinary international team demonstrated through botanical examination, phytochemical investigation, and genetic deoxyribonucleic acid analysis by polymerase chain reaction that this material contained tetrahydrocannabinol, the psychoactive component of cannabis, its oxidative degradation product, cannabinol, other metabolites, and its synthetic enzyme, tetrahydrocannabinolic acid synthase, as well as a novel genetic variant with two single nucleotide polymorphisms. The cannabis was presumably employed by this culture as a medicinal or psychoactive agent, or an aid to divination. To our knowledge, these investigations provide the oldest documentation of cannabis as a pharmacologically active agent, and contribute to the medical and archaeological record of this pre-Silk Road culture. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/19036842/abstract/Phytochemical_and_genetic_analyses_of_ancient_cannabis_from_Central_Asia_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ern260 ER -