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TGF-beta's delay skeletal muscle progenitor cell differentiation in an isoform-independent manner.
Exp Cell Res. 2009 Feb 01; 315(3):373-84.EC

Abstract

Satellite cells are a quiescent heterogeneous population of mononuclear stem and progenitor cells which, once activated, differentiate into myotubes and facilitate skeletal muscle repair or growth. The Transforming Growth Factor-beta (TGF-beta) superfamily members are elevated post-injury and their importance in the regulation of myogenesis and wound healing has been demonstrated both in vitro and in vivo. Most studies suggest a negative role for TGF-beta on satellite cell differentiation. However, none have compared the effect of these three isoforms on myogenesis in vitro. This is despite known isoform-specific effects of TGF-beta1, -beta2 and -beta3 on wound repair in other tissues. In the current study we compared the effect of TGF-beta1, -beta2 and -beta3 on proliferation and differentiation of the C2C12 myoblast cell-line. We found that, irrespective of the isoform, TGF-beta increased proliferation of C2C12 cells by changing the cellular localisation of PCNA to promote cell division and prevent cell cycle exit. Concomitantly, TGF-beta1, -beta2 and -beta3 delayed myogenic commitment by increasing MyoD degradation and decreasing myogenin expression. Terminal differentiation, as measured by a decrease in myosin heavy chain (MHC) expression, was also delayed. These results demonstrate that TGF-beta promotes proliferation and delays differentiation of C2C12 myoblasts in an isoform-independent manner.

Authors+Show Affiliations

Department of Physiological Sciences, University of Stellenbosch, Private Bag X1, Stellenbosch 7602, South Africa.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19038250

Citation

Schabort, Elske J., et al. "TGF-beta's Delay Skeletal Muscle Progenitor Cell Differentiation in an Isoform-independent Manner." Experimental Cell Research, vol. 315, no. 3, 2009, pp. 373-84.
Schabort EJ, van der Merwe M, Loos B, et al. TGF-beta's delay skeletal muscle progenitor cell differentiation in an isoform-independent manner. Exp Cell Res. 2009;315(3):373-84.
Schabort, E. J., van der Merwe, M., Loos, B., Moore, F. P., & Niesler, C. U. (2009). TGF-beta's delay skeletal muscle progenitor cell differentiation in an isoform-independent manner. Experimental Cell Research, 315(3), 373-84. https://doi.org/10.1016/j.yexcr.2008.10.037
Schabort EJ, et al. TGF-beta's Delay Skeletal Muscle Progenitor Cell Differentiation in an Isoform-independent Manner. Exp Cell Res. 2009 Feb 1;315(3):373-84. PubMed PMID: 19038250.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TGF-beta's delay skeletal muscle progenitor cell differentiation in an isoform-independent manner. AU - Schabort,Elske J, AU - van der Merwe,Mathilde, AU - Loos,Benjamin, AU - Moore,Frances P, AU - Niesler,Carola U, Y1 - 2008/11/07/ PY - 2008/04/24/received PY - 2008/10/20/revised PY - 2008/10/28/accepted PY - 2008/11/29/entrez PY - 2008/11/29/pubmed PY - 2009/2/28/medline SP - 373 EP - 84 JF - Experimental cell research JO - Exp Cell Res VL - 315 IS - 3 N2 - Satellite cells are a quiescent heterogeneous population of mononuclear stem and progenitor cells which, once activated, differentiate into myotubes and facilitate skeletal muscle repair or growth. The Transforming Growth Factor-beta (TGF-beta) superfamily members are elevated post-injury and their importance in the regulation of myogenesis and wound healing has been demonstrated both in vitro and in vivo. Most studies suggest a negative role for TGF-beta on satellite cell differentiation. However, none have compared the effect of these three isoforms on myogenesis in vitro. This is despite known isoform-specific effects of TGF-beta1, -beta2 and -beta3 on wound repair in other tissues. In the current study we compared the effect of TGF-beta1, -beta2 and -beta3 on proliferation and differentiation of the C2C12 myoblast cell-line. We found that, irrespective of the isoform, TGF-beta increased proliferation of C2C12 cells by changing the cellular localisation of PCNA to promote cell division and prevent cell cycle exit. Concomitantly, TGF-beta1, -beta2 and -beta3 delayed myogenic commitment by increasing MyoD degradation and decreasing myogenin expression. Terminal differentiation, as measured by a decrease in myosin heavy chain (MHC) expression, was also delayed. These results demonstrate that TGF-beta promotes proliferation and delays differentiation of C2C12 myoblasts in an isoform-independent manner. SN - 1090-2422 UR - https://www.unboundmedicine.com/medline/citation/19038250/TGF_beta's_delay_skeletal_muscle_progenitor_cell_differentiation_in_an_isoform_independent_manner_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4827(08)00462-X DB - PRIME DP - Unbound Medicine ER -