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Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: implications for immunoadjuvant potential.
J Ethnopharmacol. 2009 Jan 21; 121(2):241-7.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Roots of Asparagus racemosus Willd (Shatavari in vernacular) are widely used in Ayurveda as Rasayana for immunostimulation, galactogogue as also in treatment of conditions like ulcers and cancer. Various studies have indicated immunomodulatory properties of Shatavari root extracts and formulations.

AIM OF THE STUDY

To study the effect of standardized Asparagus racemosus root aqueous extract (ARE) on systemic Th1/Th2 immunity of SRBC sensitized animals.

MATERIALS AND METHODS

We used HPTLC to quantify steroidal saponins (Shatavarin IV, Immunoside) and flow cytometry to study effects of ARE on Th1/Th2 immunity. SRBC specific antibody titres and DTH responses were also monitored as markers of Th2 and Th1 responses, respectively. We also studied lymphocyte proliferation. Cyclosporin, cyclophosphamide and levamisole were used as controls.

RESULTS

Treatment with ARE (100mg/(kg b.w.p.o.)) resulted in significant increase of CD3(+) and CD4/CD8(+) percentages suggesting its effect on T cell activation. ARE treated animals showed significant up-regulation of Th1 (IL-2, IFN-g) and Th2 (IL-4) cytokines suggesting its mixed Th1/Th2 adjuvant activity. Consistent to this, ARE also showed higher antibody titres and DTH responses. ARE, in combination with LPS, Con A or SRBC, produced a significant proliferation suggesting effect on activated lymphocytes.

CONCLUSION

The study suggests mixed Th1/Th2 activity of ARE supports its immunoadjuvant potential.

Authors+Show Affiliations

Bioprospecting Laboratory, Interdisciplinary School of Health Sciences, University of Pune, Pune 411007, Maharashtra, India. manish_gautam2003@yahoo.co.inNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19038322

Citation

Gautam, Manish, et al. "Immunomodulatory Activity of Asparagus Racemosus On Systemic Th1/Th2 Immunity: Implications for Immunoadjuvant Potential." Journal of Ethnopharmacology, vol. 121, no. 2, 2009, pp. 241-7.
Gautam M, Saha S, Bani S, et al. Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: implications for immunoadjuvant potential. J Ethnopharmacol. 2009;121(2):241-7.
Gautam, M., Saha, S., Bani, S., Kaul, A., Mishra, S., Patil, D., Satti, N. K., Suri, K. A., Gairola, S., Suresh, K., Jadhav, S., Qazi, G. N., & Patwardhan, B. (2009). Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: implications for immunoadjuvant potential. Journal of Ethnopharmacology, 121(2), 241-7. https://doi.org/10.1016/j.jep.2008.10.028
Gautam M, et al. Immunomodulatory Activity of Asparagus Racemosus On Systemic Th1/Th2 Immunity: Implications for Immunoadjuvant Potential. J Ethnopharmacol. 2009 Jan 21;121(2):241-7. PubMed PMID: 19038322.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: implications for immunoadjuvant potential. AU - Gautam,Manish, AU - Saha,Santanu, AU - Bani,Sarang, AU - Kaul,A, AU - Mishra,Sanjay, AU - Patil,Dada, AU - Satti,N K, AU - Suri,K A, AU - Gairola,Sunil, AU - Suresh,K, AU - Jadhav,Suresh, AU - Qazi,G N, AU - Patwardhan,Bhushan, Y1 - 2008/11/08/ PY - 2008/03/30/received PY - 2008/09/18/revised PY - 2008/10/23/accepted PY - 2008/11/29/pubmed PY - 2009/4/2/medline PY - 2008/11/29/entrez SP - 241 EP - 7 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 121 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Roots of Asparagus racemosus Willd (Shatavari in vernacular) are widely used in Ayurveda as Rasayana for immunostimulation, galactogogue as also in treatment of conditions like ulcers and cancer. Various studies have indicated immunomodulatory properties of Shatavari root extracts and formulations. AIM OF THE STUDY: To study the effect of standardized Asparagus racemosus root aqueous extract (ARE) on systemic Th1/Th2 immunity of SRBC sensitized animals. MATERIALS AND METHODS: We used HPTLC to quantify steroidal saponins (Shatavarin IV, Immunoside) and flow cytometry to study effects of ARE on Th1/Th2 immunity. SRBC specific antibody titres and DTH responses were also monitored as markers of Th2 and Th1 responses, respectively. We also studied lymphocyte proliferation. Cyclosporin, cyclophosphamide and levamisole were used as controls. RESULTS: Treatment with ARE (100mg/(kg b.w.p.o.)) resulted in significant increase of CD3(+) and CD4/CD8(+) percentages suggesting its effect on T cell activation. ARE treated animals showed significant up-regulation of Th1 (IL-2, IFN-g) and Th2 (IL-4) cytokines suggesting its mixed Th1/Th2 adjuvant activity. Consistent to this, ARE also showed higher antibody titres and DTH responses. ARE, in combination with LPS, Con A or SRBC, produced a significant proliferation suggesting effect on activated lymphocytes. CONCLUSION: The study suggests mixed Th1/Th2 activity of ARE supports its immunoadjuvant potential. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/19038322/Immunomodulatory_activity_of_Asparagus_racemosus_on_systemic_Th1/Th2_immunity:_implications_for_immunoadjuvant_potential_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(08)00592-8 DB - PRIME DP - Unbound Medicine ER -