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Proteomic analysis of homocysteine induced proliferation of cultured neonatal rat vascular smooth muscle cells.
Biochim Biophys Acta. 2009 Feb; 1794(2):177-84.BB

Abstract

Hyperhomocysteinemia is a common independent risk factor for cardiovascular diseases. The promoting effect of homocysteine (Hcy) on vascular smooth muscle cell (VSMC) proliferation has been considered as one of the important pathological bases of atherosclerosis. However, the mechanism of VSMC proliferation induced by Hcy remains unclear. The present research used proteomic techniques to globally analyze the protein changes in proliferative VSMCs. After comparing the protein expression profiles of VSMCs between the Hcy-treated and non-treated groups, 11 protein spots were found altered markedly in proliferative VSMCs with expression of eight protein spots increased and three protein spots decreased. In the differentially expressed proteins, eight protein spots were identified successfully including glycolytic metabolism proteins: pyruvate kinase M2 (PKM2), triosephosphate isomerase (TPI) and aldose reductase (AR); cytoskeletal proteins: lamin C and vimentin; and three other proteins: calreticulin; similar to WDR1 protein and LIM and SH3 protein 1. The differentially expressed proteins were further validated by Western blot and confirmed by assay of enzymes' activities and ATP content. These results may provide some clues for comprehensively understanding the mechanism of VSMC proliferation and pathogenesis of atherosclerosis induced by Hcy.

Authors+Show Affiliations

Department of Stress Medicine, Institute of Health and Environmental Medicine, Tianjin 300050, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19038367

Citation

Liu, Xiaohua, et al. "Proteomic Analysis of Homocysteine Induced Proliferation of Cultured Neonatal Rat Vascular Smooth Muscle Cells." Biochimica Et Biophysica Acta, vol. 1794, no. 2, 2009, pp. 177-84.
Liu X, Shen J, Zhan R, et al. Proteomic analysis of homocysteine induced proliferation of cultured neonatal rat vascular smooth muscle cells. Biochim Biophys Acta. 2009;1794(2):177-84.
Liu, X., Shen, J., Zhan, R., Wang, X., Wang, X., Zhang, Z., Leng, X., Yang, Z., & Qian, L. (2009). Proteomic analysis of homocysteine induced proliferation of cultured neonatal rat vascular smooth muscle cells. Biochimica Et Biophysica Acta, 1794(2), 177-84. https://doi.org/10.1016/j.bbapap.2008.10.001
Liu X, et al. Proteomic Analysis of Homocysteine Induced Proliferation of Cultured Neonatal Rat Vascular Smooth Muscle Cells. Biochim Biophys Acta. 2009;1794(2):177-84. PubMed PMID: 19038367.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic analysis of homocysteine induced proliferation of cultured neonatal rat vascular smooth muscle cells. AU - Liu,Xiaohua, AU - Shen,Jing, AU - Zhan,Rui, AU - Wang,Xingxing, AU - Wang,Xiaoming, AU - Zhang,Zhiqing, AU - Leng,Xue, AU - Yang,Zhihua, AU - Qian,Lingjia, Y1 - 2008/10/21/ PY - 2008/04/18/received PY - 2008/09/08/revised PY - 2008/10/03/accepted PY - 2008/11/29/pubmed PY - 2009/3/24/medline PY - 2008/11/29/entrez SP - 177 EP - 84 JF - Biochimica et biophysica acta JO - Biochim. Biophys. Acta VL - 1794 IS - 2 N2 - Hyperhomocysteinemia is a common independent risk factor for cardiovascular diseases. The promoting effect of homocysteine (Hcy) on vascular smooth muscle cell (VSMC) proliferation has been considered as one of the important pathological bases of atherosclerosis. However, the mechanism of VSMC proliferation induced by Hcy remains unclear. The present research used proteomic techniques to globally analyze the protein changes in proliferative VSMCs. After comparing the protein expression profiles of VSMCs between the Hcy-treated and non-treated groups, 11 protein spots were found altered markedly in proliferative VSMCs with expression of eight protein spots increased and three protein spots decreased. In the differentially expressed proteins, eight protein spots were identified successfully including glycolytic metabolism proteins: pyruvate kinase M2 (PKM2), triosephosphate isomerase (TPI) and aldose reductase (AR); cytoskeletal proteins: lamin C and vimentin; and three other proteins: calreticulin; similar to WDR1 protein and LIM and SH3 protein 1. The differentially expressed proteins were further validated by Western blot and confirmed by assay of enzymes' activities and ATP content. These results may provide some clues for comprehensively understanding the mechanism of VSMC proliferation and pathogenesis of atherosclerosis induced by Hcy. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/19038367/Proteomic_analysis_of_homocysteine_induced_proliferation_of_cultured_neonatal_rat_vascular_smooth_muscle_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-9639(08)00306-3 DB - PRIME DP - Unbound Medicine ER -