Tags

Type your tag names separated by a space and hit enter

The GnRH antagonist reduces chemotherapy-induced ovarian damage in rats by suppressing the apoptosis.
Gynecol Oncol 2009; 112(2):409-14GO

Abstract

OBJECTIVE

GnRH antagonist cetrorelix could reserve the ovarian follicles during chemotherapy, but the mechanism remains unclear. The objectives of this study were to examine the overall effect of cetrorelix against ovarian failure and to define if the apoptotic process was involved.

METHODS

Female SD rats were injected with cetrorelix before and after administration of saline, or cyclophosphamide (Cy), or oral etoposide (VP). Main outcome measures were the number of ovarian follicles, serum hormones, ovary histology and apoptotic markers.

RESULTS

The females exposed to Cy or VP had reduced body and ovary weights, which could be restored by cetrorelix pretreatment. Single cetrorelix treatment could increase the number of primordial follicles, but reduce the number of growing and mature follicles. As a consequence, the ovaries exposed to cetrorelix prior to Cy or VP showed significantly higher numbers of follicles at all developing stages than those exposed to Cy or VP alone. Meanwhile, the ovarian apoptotic indexes as shown by TUNEL assay were reduced by cetrorelix pretreatment and the ovary expressed less caspases-3 and more Bcl-2 compared with chemotherapy alone. Moreover, the rats regained normal hormonal profile after cetrorelix pretreatment without any alterations in ovarian expression of estrogen receptor (ER)alpha, ERbeta, or progesterone receptor (PR).

CONCLUSION

Cetrorelix could reduce the chemotherapy-induced ovarian damage through regulating the expression of Bcl-2 and caspases-3 in the ovary, without any expressional alterations of nuclear receptors, suggesting the apoptosis pathway involved.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Xi'jing Hospital, PR China. huangyh@fmmu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19038435

Citation

Huang, Yan-hong, et al. "The GnRH Antagonist Reduces Chemotherapy-induced Ovarian Damage in Rats By Suppressing the Apoptosis." Gynecologic Oncology, vol. 112, no. 2, 2009, pp. 409-14.
Huang YH, Zhao XJ, Zhang QH, et al. The GnRH antagonist reduces chemotherapy-induced ovarian damage in rats by suppressing the apoptosis. Gynecol Oncol. 2009;112(2):409-14.
Huang, Y. H., Zhao, X. J., Zhang, Q. H., & Xin, X. Y. (2009). The GnRH antagonist reduces chemotherapy-induced ovarian damage in rats by suppressing the apoptosis. Gynecologic Oncology, 112(2), pp. 409-14. doi:10.1016/j.ygyno.2008.09.044.
Huang YH, et al. The GnRH Antagonist Reduces Chemotherapy-induced Ovarian Damage in Rats By Suppressing the Apoptosis. Gynecol Oncol. 2009;112(2):409-14. PubMed PMID: 19038435.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The GnRH antagonist reduces chemotherapy-induced ovarian damage in rats by suppressing the apoptosis. AU - Huang,Yan-hong, AU - Zhao,Xue-jing, AU - Zhang,Qing-hong, AU - Xin,Xiao-yan, Y1 - 2008/11/26/ PY - 2008/07/29/received PY - 2008/09/11/revised PY - 2008/09/19/accepted PY - 2008/11/29/entrez PY - 2008/11/29/pubmed PY - 2009/1/27/medline SP - 409 EP - 14 JF - Gynecologic oncology JO - Gynecol. Oncol. VL - 112 IS - 2 N2 - OBJECTIVE: GnRH antagonist cetrorelix could reserve the ovarian follicles during chemotherapy, but the mechanism remains unclear. The objectives of this study were to examine the overall effect of cetrorelix against ovarian failure and to define if the apoptotic process was involved. METHODS: Female SD rats were injected with cetrorelix before and after administration of saline, or cyclophosphamide (Cy), or oral etoposide (VP). Main outcome measures were the number of ovarian follicles, serum hormones, ovary histology and apoptotic markers. RESULTS: The females exposed to Cy or VP had reduced body and ovary weights, which could be restored by cetrorelix pretreatment. Single cetrorelix treatment could increase the number of primordial follicles, but reduce the number of growing and mature follicles. As a consequence, the ovaries exposed to cetrorelix prior to Cy or VP showed significantly higher numbers of follicles at all developing stages than those exposed to Cy or VP alone. Meanwhile, the ovarian apoptotic indexes as shown by TUNEL assay were reduced by cetrorelix pretreatment and the ovary expressed less caspases-3 and more Bcl-2 compared with chemotherapy alone. Moreover, the rats regained normal hormonal profile after cetrorelix pretreatment without any alterations in ovarian expression of estrogen receptor (ER)alpha, ERbeta, or progesterone receptor (PR). CONCLUSION: Cetrorelix could reduce the chemotherapy-induced ovarian damage through regulating the expression of Bcl-2 and caspases-3 in the ovary, without any expressional alterations of nuclear receptors, suggesting the apoptosis pathway involved. SN - 1095-6859 UR - https://www.unboundmedicine.com/medline/citation/19038435/The_GnRH_antagonist_reduces_chemotherapy_induced_ovarian_damage_in_rats_by_suppressing_the_apoptosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0090-8258(08)00790-7 DB - PRIME DP - Unbound Medicine ER -