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Peripheral antinociceptive effects of mu- and delta-opioid receptor agonists in NOS2 and NOS1 knockout mice during chronic inflammatory pain.
Eur J Pharmacol. 2009 Jan 05; 602(1):41-9.EJ

Abstract

The aim of this study is to investigate the involvement of nitric oxide synthesized by the inducible (NOS2) or neuronal (NOS1) nitric oxide synthases in the local antinociceptive effects produced by micro- and delta-opioid receptor agonists during chronic inflammatory pain. Peripheral inflammatory pain was induced in NOS2 and NOS1 knockout mice and their wild type littermates by the subplantar administration of complete Freund's adjuvant (30 microl). The presence of paw inflammation, mechanical allodynia and thermal hyperalgesia induced by complete Freund's adjuvant were assessed by measuring paw diameter and using the von Frey filaments and plantar tests, respectively. During chronic inflammation, NOS2 deficient mice have a more rapid recovery of paw edema and a reduced thermal hyperalgesia compared to wild type. In contrast, a reduced paw edema and mechanical allodynia, as well as a modest rapid recovery from thermal hyperalgesia were observed in NOS1 knockout mice compared to wild type. The thermal hyperalgesia induced by complete Freund's adjuvant was not completely reversed by the subplantar administration of morphine (days 4 and 7) or [D-Pen (2,5)] enkephalin (DPDPE) (days 1 and 4) in NOS2 knockout mice as occurs in wild type mice. Moreover, the local administration of morphine or DPDPE also failed to reverse the decrease of ipsilateral paw withdrawal latency induced by complete Freund's adjuvant in NOS1 knockout mice throughout 10 days of peripheral inflammation. These results indicate the different roles played by nitric oxide synthesized by NOS2 or NOS1 in the maintenance of mechanical allodynia and thermal hyperalgesia induced by chronic inflammatory pain as well as, in the antinociceptive effects produced by micro- and delta-opioid receptor agonists during peripheral inflammatory pain.

Authors+Show Affiliations

Laboratori de Neurofarmacologia Molecular, Institut de Recerca Hospital de Sta Creu i Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19041302

Citation

Leánez, Sergi, et al. "Peripheral Antinociceptive Effects of Mu- and Delta-opioid Receptor Agonists in NOS2 and NOS1 Knockout Mice During Chronic Inflammatory Pain." European Journal of Pharmacology, vol. 602, no. 1, 2009, pp. 41-9.
Leánez S, Hervera A, Pol O. Peripheral antinociceptive effects of mu- and delta-opioid receptor agonists in NOS2 and NOS1 knockout mice during chronic inflammatory pain. Eur J Pharmacol. 2009;602(1):41-9.
Leánez, S., Hervera, A., & Pol, O. (2009). Peripheral antinociceptive effects of mu- and delta-opioid receptor agonists in NOS2 and NOS1 knockout mice during chronic inflammatory pain. European Journal of Pharmacology, 602(1), 41-9. https://doi.org/10.1016/j.ejphar.2008.11.019
Leánez S, Hervera A, Pol O. Peripheral Antinociceptive Effects of Mu- and Delta-opioid Receptor Agonists in NOS2 and NOS1 Knockout Mice During Chronic Inflammatory Pain. Eur J Pharmacol. 2009 Jan 5;602(1):41-9. PubMed PMID: 19041302.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Peripheral antinociceptive effects of mu- and delta-opioid receptor agonists in NOS2 and NOS1 knockout mice during chronic inflammatory pain. AU - Leánez,Sergi, AU - Hervera,Arnau, AU - Pol,Olga, Y1 - 2008/11/18/ PY - 2008/09/11/received PY - 2008/10/27/revised PY - 2008/11/10/accepted PY - 2008/12/2/entrez PY - 2008/12/2/pubmed PY - 2009/10/8/medline SP - 41 EP - 9 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 602 IS - 1 N2 - The aim of this study is to investigate the involvement of nitric oxide synthesized by the inducible (NOS2) or neuronal (NOS1) nitric oxide synthases in the local antinociceptive effects produced by micro- and delta-opioid receptor agonists during chronic inflammatory pain. Peripheral inflammatory pain was induced in NOS2 and NOS1 knockout mice and their wild type littermates by the subplantar administration of complete Freund's adjuvant (30 microl). The presence of paw inflammation, mechanical allodynia and thermal hyperalgesia induced by complete Freund's adjuvant were assessed by measuring paw diameter and using the von Frey filaments and plantar tests, respectively. During chronic inflammation, NOS2 deficient mice have a more rapid recovery of paw edema and a reduced thermal hyperalgesia compared to wild type. In contrast, a reduced paw edema and mechanical allodynia, as well as a modest rapid recovery from thermal hyperalgesia were observed in NOS1 knockout mice compared to wild type. The thermal hyperalgesia induced by complete Freund's adjuvant was not completely reversed by the subplantar administration of morphine (days 4 and 7) or [D-Pen (2,5)] enkephalin (DPDPE) (days 1 and 4) in NOS2 knockout mice as occurs in wild type mice. Moreover, the local administration of morphine or DPDPE also failed to reverse the decrease of ipsilateral paw withdrawal latency induced by complete Freund's adjuvant in NOS1 knockout mice throughout 10 days of peripheral inflammation. These results indicate the different roles played by nitric oxide synthesized by NOS2 or NOS1 in the maintenance of mechanical allodynia and thermal hyperalgesia induced by chronic inflammatory pain as well as, in the antinociceptive effects produced by micro- and delta-opioid receptor agonists during peripheral inflammatory pain. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/19041302/Peripheral_antinociceptive_effects_of_mu__and_delta_opioid_receptor_agonists_in_NOS2_and_NOS1_knockout_mice_during_chronic_inflammatory_pain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(08)01174-6 DB - PRIME DP - Unbound Medicine ER -