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Potential anticancer properties of the water extract of Inonotus [corrected] obliquus by induction of apoptosis in melanoma B16-F10 cells.
J Ethnopharmacol. 2009 Jan 21; 121(2):221-8.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Inonotus obliquus (Chaga mushroom), one of the widely known medicinal mushrooms, has been used to treat various cancers in Russia and most of Baltic countries for many centuries.

AIM OF THE STUDY

To examine the anti-proliferative effects of Inonotus obliquus extract on melanoma B16-F10 cells. Furthermore, to assess the anti-tumor effect of Inonotus obliquus extract in vivo in Balb/c mice.

MATERIALS AND METHODS

The water extract of Inonotus obliquus was studied for anti-proliferative effects on the growth and morphology of B16-F10 melanoma cells and for anti-tumor effect using in vivo in Balb/c mice.

RESULTS

Inonotus obliquus extract not only inhibited the growth of B16-F10 cells by causing cell cycle arrest at G(0)/G(1) phase and apoptosis, but also induced cell differentiation. These effects were associated with the down-regulation of pRb, p53 and p27 expression levels, and further showed that Inonotus obliquus extract resulted in a G(0)/G(1) cell cycle arrest with reduction of cyclin E/D1 and Cdk 2/4 expression levels. Furthermore, the anti-tumor effect of Inonotus obliquus extract was assessed in vivo in Balb/c mice. Intraperitoneal administration of Inonotus obliquus extract significantly inhibited the growth of tumor mass in B16-F10 cells implanted mice, resulting in a 3-fold (relative to the positive control, (*)p<0.05) inhibit at dose of 20mg/kg/day for 10 days.

CONCLUSION

This study showed that the water extract of Inonotus obliquus mushroom exhibited a potential anticancer activity against B16-F10 melanoma cells in vitro and in vivo through the inhibition of proliferation and induction of differentiation and apoptosis of cancer cells.

Authors+Show Affiliations

VestibuloCochlear Research Center and Department of Microbiology, Wonkwang University, Iksan, Jeonbuk 570-749, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19041933

Citation

Youn, Myung-Ja, et al. "Potential Anticancer Properties of the Water Extract of Inonotus [corrected] Obliquus By Induction of Apoptosis in Melanoma B16-F10 Cells." Journal of Ethnopharmacology, vol. 121, no. 2, 2009, pp. 221-8.
Youn MJ, Kim JK, Park SY, et al. Potential anticancer properties of the water extract of Inonotus [corrected] obliquus by induction of apoptosis in melanoma B16-F10 cells. J Ethnopharmacol. 2009;121(2):221-8.
Youn, M. J., Kim, J. K., Park, S. Y., Kim, Y., Park, C., Kim, E. S., Park, K. I., So, H. S., & Park, R. (2009). Potential anticancer properties of the water extract of Inonotus [corrected] obliquus by induction of apoptosis in melanoma B16-F10 cells. Journal of Ethnopharmacology, 121(2), 221-8. https://doi.org/10.1016/j.jep.2008.10.016
Youn MJ, et al. Potential Anticancer Properties of the Water Extract of Inonotus [corrected] Obliquus By Induction of Apoptosis in Melanoma B16-F10 Cells. J Ethnopharmacol. 2009 Jan 21;121(2):221-8. PubMed PMID: 19041933.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potential anticancer properties of the water extract of Inonotus [corrected] obliquus by induction of apoptosis in melanoma B16-F10 cells. AU - Youn,Myung-Ja, AU - Kim,Jin-Kyung, AU - Park,Seong-Yeol, AU - Kim,Yunha, AU - Park,Channy, AU - Kim,Eun Sook, AU - Park,Kie-In, AU - So,Hong Seob, AU - Park,Raekil, Y1 - 2008/10/25/ PY - 2007/09/08/received PY - 2008/09/24/revised PY - 2008/10/03/accepted PY - 2008/12/2/pubmed PY - 2009/4/2/medline PY - 2008/12/2/entrez SP - 221 EP - 8 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 121 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Inonotus obliquus (Chaga mushroom), one of the widely known medicinal mushrooms, has been used to treat various cancers in Russia and most of Baltic countries for many centuries. AIM OF THE STUDY: To examine the anti-proliferative effects of Inonotus obliquus extract on melanoma B16-F10 cells. Furthermore, to assess the anti-tumor effect of Inonotus obliquus extract in vivo in Balb/c mice. MATERIALS AND METHODS: The water extract of Inonotus obliquus was studied for anti-proliferative effects on the growth and morphology of B16-F10 melanoma cells and for anti-tumor effect using in vivo in Balb/c mice. RESULTS: Inonotus obliquus extract not only inhibited the growth of B16-F10 cells by causing cell cycle arrest at G(0)/G(1) phase and apoptosis, but also induced cell differentiation. These effects were associated with the down-regulation of pRb, p53 and p27 expression levels, and further showed that Inonotus obliquus extract resulted in a G(0)/G(1) cell cycle arrest with reduction of cyclin E/D1 and Cdk 2/4 expression levels. Furthermore, the anti-tumor effect of Inonotus obliquus extract was assessed in vivo in Balb/c mice. Intraperitoneal administration of Inonotus obliquus extract significantly inhibited the growth of tumor mass in B16-F10 cells implanted mice, resulting in a 3-fold (relative to the positive control, (*)p<0.05) inhibit at dose of 20mg/kg/day for 10 days. CONCLUSION: This study showed that the water extract of Inonotus obliquus mushroom exhibited a potential anticancer activity against B16-F10 melanoma cells in vitro and in vivo through the inhibition of proliferation and induction of differentiation and apoptosis of cancer cells. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/19041933/Potential_anticancer_properties_of_the_water_extract_of_Inonotus_[corrected]_obliquus_by_induction_of_apoptosis_in_melanoma_B16_F10_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(08)00557-6 DB - PRIME DP - Unbound Medicine ER -