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A novel label-free multi-throughput optical biosensor based on localized surface plasmon resonance.
Biosens Bioelectron. 2009 Mar 15; 24(7):2255-9.BB

Abstract

A novel and sensitive multi-throughput localized surface plasmon resonance (MLSPR) biosensor was developed for the first time. Various gold nanorods with different aspect ratios were used to fabricate the optical sensor. Five kinds of gold nanorods with different aspect ratios were chosen to construct five throughputs of MLSPR. Various LSPR peaks imply that different acceptor-ligand pairs can be detected simultaneously in the wavelength range from 530 to 940nm. The biosensor immobilized on glass slides was applied to label-free detection between acceptor and ligand. The MLSPR-based optical biosensor can be used to detect three antigen-antibody pairs simultaneously. The biosensor proposed herein is easy to fabricate, and its operation procedure is convenient as labeling procedure is unnecessary.

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Authors+Show Affiliations

Huang H
School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, China.
He C
No affiliation info available
Zeng Y
No affiliation info available
Xia X
No affiliation info available
Yu X
No affiliation info available
Yi P
No affiliation info available
Chen Z
No affiliation info available

MeSH

Biosensing TechniquesEquipment DesignEquipment Failure AnalysisImmunoassayOptical DevicesProtein Interaction MappingReproducibility of ResultsSensitivity and SpecificityStaining and LabelingSurface Plasmon Resonance

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19042120

Citation

Huang, Haowen, et al. "A Novel Label-free Multi-throughput Optical Biosensor Based On Localized Surface Plasmon Resonance." Biosensors & Bioelectronics, vol. 24, no. 7, 2009, pp. 2255-9.
Huang H, He C, Zeng Y, et al. A novel label-free multi-throughput optical biosensor based on localized surface plasmon resonance. Biosens Bioelectron. 2009;24(7):2255-9.
Huang, H., He, C., Zeng, Y., Xia, X., Yu, X., Yi, P., & Chen, Z. (2009). A novel label-free multi-throughput optical biosensor based on localized surface plasmon resonance. Biosensors & Bioelectronics, 24(7), 2255-9. https://doi.org/10.1016/j.bios.2008.10.013
Huang H, et al. A Novel Label-free Multi-throughput Optical Biosensor Based On Localized Surface Plasmon Resonance. Biosens Bioelectron. 2009 Mar 15;24(7):2255-9. PubMed PMID: 19042120.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel label-free multi-throughput optical biosensor based on localized surface plasmon resonance. AU - Huang,Haowen, AU - He,Chaocai, AU - Zeng,Yunlong, AU - Xia,Xiaodong, AU - Yu,Xianyong, AU - Yi,Pinggui, AU - Chen,Zhong, Y1 - 2008/10/25/ PY - 2008/09/16/received PY - 2008/10/09/accepted PY - 2008/12/2/pubmed PY - 2009/5/27/medline PY - 2008/12/2/entrez SP - 2255 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 7 N2 - A novel and sensitive multi-throughput localized surface plasmon resonance (MLSPR) biosensor was developed for the first time. Various gold nanorods with different aspect ratios were used to fabricate the optical sensor. Five kinds of gold nanorods with different aspect ratios were chosen to construct five throughputs of MLSPR. Various LSPR peaks imply that different acceptor-ligand pairs can be detected simultaneously in the wavelength range from 530 to 940nm. The biosensor immobilized on glass slides was applied to label-free detection between acceptor and ligand. The MLSPR-based optical biosensor can be used to detect three antigen-antibody pairs simultaneously. The biosensor proposed herein is easy to fabricate, and its operation procedure is convenient as labeling procedure is unnecessary. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/19042120/A_novel_label_free_multi_throughput_optical_biosensor_based_on_localized_surface_plasmon_resonance_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00584-8 DB - PRIME DP - Unbound Medicine ER -