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The endocannabinoid system in bull sperm and bovine oviductal epithelium: role of anandamide in sperm-oviduct interaction.
Reproduction. 2009 Mar; 137(3):403-14.R

Abstract

Anandamide binds to cannabinoid receptors and plays several central and peripheral functions. The aim of this work was to study the possible role for this endocannabinoid in controlling sperm-oviduct interaction in mammals. We observed that bull sperm and bovine oviductal epithelial cells express cannabinoid receptors, CB1 and CB2, and fatty acid amide hydrolase, the enzyme that controls intracellular anandamide levels. A quantitative assay to determine whether anandamide was involved in bovine sperm-oviduct interaction was developed. R(+)-methanandamide, a non-hydrolysable anandamide analog, inhibited sperm binding to and induced sperm release from oviductal epithelia. Selective CB1 antagonists (SR141716A or AM251) completely blocked R(+)-methanandamide effects. However, SR144528, a selective CB2 antagonist, did not exert any effect, indicating that only CB1 was involved in R(+)-methanandamide effect. This effect was not caused by inhibition of the sperm progressive motility or by induction of the acrosome reaction. Overall, our findings indicate for the first time that the endocannabinoid system is present in bovine sperm and oviductal epithelium and that anandamide modulates the sperm-oviduct interaction, by inhibition of sperm binding and induction of sperm release from oviductal epithelial cells, probably by activating CB1 receptors.

Authors+Show Affiliations

Facultad de Medicina, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires, Buenos Aires, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19042982

Citation

Gervasi, María Gracia, et al. "The Endocannabinoid System in Bull Sperm and Bovine Oviductal Epithelium: Role of Anandamide in Sperm-oviduct Interaction." Reproduction (Cambridge, England), vol. 137, no. 3, 2009, pp. 403-14.
Gervasi MG, Rapanelli M, Ribeiro ML, et al. The endocannabinoid system in bull sperm and bovine oviductal epithelium: role of anandamide in sperm-oviduct interaction. Reproduction. 2009;137(3):403-14.
Gervasi, M. G., Rapanelli, M., Ribeiro, M. L., Farina, M., Billi, S., Franchi, A. M., & Perez Martinez, S. (2009). The endocannabinoid system in bull sperm and bovine oviductal epithelium: role of anandamide in sperm-oviduct interaction. Reproduction (Cambridge, England), 137(3), 403-14. https://doi.org/10.1530/REP-08-0204
Gervasi MG, et al. The Endocannabinoid System in Bull Sperm and Bovine Oviductal Epithelium: Role of Anandamide in Sperm-oviduct Interaction. Reproduction. 2009;137(3):403-14. PubMed PMID: 19042982.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The endocannabinoid system in bull sperm and bovine oviductal epithelium: role of anandamide in sperm-oviduct interaction. AU - Gervasi,María Gracia, AU - Rapanelli,Maximiliano, AU - Ribeiro,María Laura, AU - Farina,Mariana, AU - Billi,Silvia, AU - Franchi,Ana María, AU - Perez Martinez,Silvina, Y1 - 2008/11/28/ PY - 2008/12/2/pubmed PY - 2009/6/16/medline PY - 2008/12/2/entrez SP - 403 EP - 14 JF - Reproduction (Cambridge, England) JO - Reproduction VL - 137 IS - 3 N2 - Anandamide binds to cannabinoid receptors and plays several central and peripheral functions. The aim of this work was to study the possible role for this endocannabinoid in controlling sperm-oviduct interaction in mammals. We observed that bull sperm and bovine oviductal epithelial cells express cannabinoid receptors, CB1 and CB2, and fatty acid amide hydrolase, the enzyme that controls intracellular anandamide levels. A quantitative assay to determine whether anandamide was involved in bovine sperm-oviduct interaction was developed. R(+)-methanandamide, a non-hydrolysable anandamide analog, inhibited sperm binding to and induced sperm release from oviductal epithelia. Selective CB1 antagonists (SR141716A or AM251) completely blocked R(+)-methanandamide effects. However, SR144528, a selective CB2 antagonist, did not exert any effect, indicating that only CB1 was involved in R(+)-methanandamide effect. This effect was not caused by inhibition of the sperm progressive motility or by induction of the acrosome reaction. Overall, our findings indicate for the first time that the endocannabinoid system is present in bovine sperm and oviductal epithelium and that anandamide modulates the sperm-oviduct interaction, by inhibition of sperm binding and induction of sperm release from oviductal epithelial cells, probably by activating CB1 receptors. SN - 1741-7899 UR - https://www.unboundmedicine.com/medline/citation/19042982/The_endocannabinoid_system_in_bull_sperm_and_bovine_oviductal_epithelium:_role_of_anandamide_in_sperm_oviduct_interaction_ L2 - https://rep.bioscientifica.com/doi/10.1530/REP-08-0204 DB - PRIME DP - Unbound Medicine ER -