Tags

Type your tag names separated by a space and hit enter

Parvovirus B19 profiles in patients presenting with acute myocarditis and chronic dilated cardiomyopathy.
Med Sci Monit. 2008 Dec; 14(12):CR589-97.MS

Abstract

BACKGROUND

Parvovirus B19 (B19V) is the most prevalent cardiotropic virus in endomyocardial biopsies (EMBs) from patients presenting with acute myocarditis (AMC) and chronic dilated cardiomyopathy (DCM). We elucidated the role of B19V specific IgG and IgM antibodies against native VP2-capsids and denatured VP1-, VP2- and NS1-proteins, which discriminate disease acuity in other B19V related diseases, in patients presenting with clinically suspected AMC and DCM for the determination of the viral infection stage.

MATERIAL/METHODS

n=62 prospectively enrolled AMC (n=33) and DCM (n=29) patients were investigated. B19V genomes were amplified in EMBs by nested PCR (nPCR). B19V-specific IgG and IgM were investigated by recomLine blots in the sera.

RESULTS

B19V genomes were detectable by nPCR with comparable frequencies in AMC (63.6%) and DCM patients (51.7%), respectively. IgM antibodies were detectable in 18.1% of the AMC, but not in DCM patients. In patients with myocardial B19V infection, antibody profiles indicating recent infections were more frequent in AMC (47.6%) compared with DCM patients (6.7%), while persistent/reactivating profiles were significantly more prevalent in DCM (20.0%) compared with AMC (0%) patients (p<0.05).

CONCLUSIONS

IgM B19V antibodies can be detected primarily in AMC, but not in DCM patients. The discrimination of the B19V specific IgG antibodies using recomLine blots may be suitable to distinguish acute versus chronic, and persistent/reactivating infection status in patients with myocardial B19V infection.

Authors+Show Affiliations

Department of Cardiology and Pneumonology, Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19043365

Citation

Escher, Felicitas, et al. "Parvovirus B19 Profiles in Patients Presenting With Acute Myocarditis and Chronic Dilated Cardiomyopathy." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 14, no. 12, 2008, pp. CR589-97.
Escher F, Modrow S, Sabi T, et al. Parvovirus B19 profiles in patients presenting with acute myocarditis and chronic dilated cardiomyopathy. Med Sci Monit. 2008;14(12):CR589-97.
Escher, F., Modrow, S., Sabi, T., Kühl, U., Lassner, D., Schultheiss, H. P., & Noutsias, M. (2008). Parvovirus B19 profiles in patients presenting with acute myocarditis and chronic dilated cardiomyopathy. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 14(12), CR589-97.
Escher F, et al. Parvovirus B19 Profiles in Patients Presenting With Acute Myocarditis and Chronic Dilated Cardiomyopathy. Med Sci Monit. 2008;14(12):CR589-97. PubMed PMID: 19043365.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Parvovirus B19 profiles in patients presenting with acute myocarditis and chronic dilated cardiomyopathy. AU - Escher,Felicitas, AU - Modrow,Susanne, AU - Sabi,Titus, AU - Kühl,Uwe, AU - Lassner,Dirk, AU - Schultheiss,Heinz-Peter, AU - Noutsias,Michel, PY - 2008/12/2/pubmed PY - 2009/2/20/medline PY - 2008/12/2/entrez SP - CR589 EP - 97 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med Sci Monit VL - 14 IS - 12 N2 - BACKGROUND: Parvovirus B19 (B19V) is the most prevalent cardiotropic virus in endomyocardial biopsies (EMBs) from patients presenting with acute myocarditis (AMC) and chronic dilated cardiomyopathy (DCM). We elucidated the role of B19V specific IgG and IgM antibodies against native VP2-capsids and denatured VP1-, VP2- and NS1-proteins, which discriminate disease acuity in other B19V related diseases, in patients presenting with clinically suspected AMC and DCM for the determination of the viral infection stage. MATERIAL/METHODS: n=62 prospectively enrolled AMC (n=33) and DCM (n=29) patients were investigated. B19V genomes were amplified in EMBs by nested PCR (nPCR). B19V-specific IgG and IgM were investigated by recomLine blots in the sera. RESULTS: B19V genomes were detectable by nPCR with comparable frequencies in AMC (63.6%) and DCM patients (51.7%), respectively. IgM antibodies were detectable in 18.1% of the AMC, but not in DCM patients. In patients with myocardial B19V infection, antibody profiles indicating recent infections were more frequent in AMC (47.6%) compared with DCM patients (6.7%), while persistent/reactivating profiles were significantly more prevalent in DCM (20.0%) compared with AMC (0%) patients (p<0.05). CONCLUSIONS: IgM B19V antibodies can be detected primarily in AMC, but not in DCM patients. The discrimination of the B19V specific IgG antibodies using recomLine blots may be suitable to distinguish acute versus chronic, and persistent/reactivating infection status in patients with myocardial B19V infection. SN - 1643-3750 UR - https://www.unboundmedicine.com/medline/citation/19043365/Parvovirus_B19_profiles_in_patients_presenting_with_acute_myocarditis_and_chronic_dilated_cardiomyopathy_ L2 - https://www.medscimonit.com/download/index/idArt/869479 DB - PRIME DP - Unbound Medicine ER -