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Effects of feeding fish oil on mesenteric lymph node cytokine responses in obese leptin receptor-deficient JCR:LA-cp rats.
Int J Obes (Lond). 2009 Jan; 33(1):96-103.IJ

Abstract

OBJECTIVE

berrant immune responses have been identified in obesity; however, immune cells of lymph nodes residing in the inflammatory environment of visceral adipose tissue have been largely overlooked. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) can reduce inflammation and modify T-cell function and therefore may improve immune function in obesity. Thus, we determined the effects of feeding fish oil (FO) containing EPA and DHA on mesenteric lymph node (MLN) immune cell function.

METHODS

In this study, 14-week-old obese, leptin receptor-deficient JCR:LA-cp rats (cp/cp) (n=10 per group) were randomized to one of three nutritionally adequate diets for 3 weeks: control (ctl, 0% EPA+DHA), low FO (LFO, 0.8% w/w EPA+DHA) or high FO (HFO, 1.4% w/w EPA+DHA). Lean JCR:LA-cp (Cp/cp or Cp/Cp) rats (n=5) were fed ctl diet. MLN cell phospholipid (PL) fatty acid composition, phenotypes and cytokine production were measured.

RESULTS

Obese ctl rats produced more IL-1beta, IL-4 and IL-10, despite a higher proportion of (n-3) polyunsaturated fatty acids (PUFAs) and a lower (n-6):(n-3) PUFA ratio in MLN PL compared with lean ctl rats (P<0.05). Concanavalin A-stimulated IL-2 production did not differ from lean rats even though obese ctl rats had a lower proportion of CD4(+)CD25(+) cells (P<0.05). Feeding FO to obese rats increased the incorporation of (n-3) PUFA into MLN PL and normalized production of IL-1beta (HFO only), IL-4 and IL-10 to the levels similar to lean ctl rats (P<0.05).

CONCLUSION

We demonstrate for the first time that obese JCR:LA-cp rats have impaired responses of MLN immune cells to mitogen stimulation and altered PL fatty acid composition. Feeding FO lowered the ex vivo inflammatory response (HFO only) and production of Th2 cytokines, without changing IL-2 production from ConA-stimulated splenocytes, which may occur independent of leptin signalling.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Ruth MR
Department of Agricultural Food and Nutritional Science, Alberta Institute for Human Nutrition, University of Alberta, Edmonton, Alberta, Canada.
Proctor SD
No affiliation info available
Field CJ
No affiliation info available

MeSH

AnimalsCells, CulturedCytokinesDocosahexaenoic AcidsEicosapentaenoic AcidFatty Acids, Omega-3Fish OilsHaptoglobinsImmunophenotypingLymph NodesMesenteryObesityPhospholipidsRandom AllocationRatsRats, Mutant StrainsReceptors, Leptin

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19048014

Citation

Ruth, M R., et al. "Effects of Feeding Fish Oil On Mesenteric Lymph Node Cytokine Responses in Obese Leptin Receptor-deficient JCR:LA-cp Rats." International Journal of Obesity (2005), vol. 33, no. 1, 2009, pp. 96-103.
Ruth MR, Proctor SD, Field CJ. Effects of feeding fish oil on mesenteric lymph node cytokine responses in obese leptin receptor-deficient JCR:LA-cp rats. Int J Obes (Lond). 2009;33(1):96-103.
Ruth, M. R., Proctor, S. D., & Field, C. J. (2009). Effects of feeding fish oil on mesenteric lymph node cytokine responses in obese leptin receptor-deficient JCR:LA-cp rats. International Journal of Obesity (2005), 33(1), 96-103. https://doi.org/10.1038/ijo.2008.227
Ruth MR, Proctor SD, Field CJ. Effects of Feeding Fish Oil On Mesenteric Lymph Node Cytokine Responses in Obese Leptin Receptor-deficient JCR:LA-cp Rats. Int J Obes (Lond). 2009;33(1):96-103. PubMed PMID: 19048014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of feeding fish oil on mesenteric lymph node cytokine responses in obese leptin receptor-deficient JCR:LA-cp rats. AU - Ruth,M R, AU - Proctor,S D, AU - Field,C J, Y1 - 2008/12/02/ PY - 2008/12/3/pubmed PY - 2009/9/17/medline PY - 2008/12/3/entrez SP - 96 EP - 103 JF - International journal of obesity (2005) JO - Int J Obes (Lond) VL - 33 IS - 1 N2 - OBJECTIVE: berrant immune responses have been identified in obesity; however, immune cells of lymph nodes residing in the inflammatory environment of visceral adipose tissue have been largely overlooked. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) can reduce inflammation and modify T-cell function and therefore may improve immune function in obesity. Thus, we determined the effects of feeding fish oil (FO) containing EPA and DHA on mesenteric lymph node (MLN) immune cell function. METHODS: In this study, 14-week-old obese, leptin receptor-deficient JCR:LA-cp rats (cp/cp) (n=10 per group) were randomized to one of three nutritionally adequate diets for 3 weeks: control (ctl, 0% EPA+DHA), low FO (LFO, 0.8% w/w EPA+DHA) or high FO (HFO, 1.4% w/w EPA+DHA). Lean JCR:LA-cp (Cp/cp or Cp/Cp) rats (n=5) were fed ctl diet. MLN cell phospholipid (PL) fatty acid composition, phenotypes and cytokine production were measured. RESULTS: Obese ctl rats produced more IL-1beta, IL-4 and IL-10, despite a higher proportion of (n-3) polyunsaturated fatty acids (PUFAs) and a lower (n-6):(n-3) PUFA ratio in MLN PL compared with lean ctl rats (P<0.05). Concanavalin A-stimulated IL-2 production did not differ from lean rats even though obese ctl rats had a lower proportion of CD4(+)CD25(+) cells (P<0.05). Feeding FO to obese rats increased the incorporation of (n-3) PUFA into MLN PL and normalized production of IL-1beta (HFO only), IL-4 and IL-10 to the levels similar to lean ctl rats (P<0.05). CONCLUSION: We demonstrate for the first time that obese JCR:LA-cp rats have impaired responses of MLN immune cells to mitogen stimulation and altered PL fatty acid composition. Feeding FO lowered the ex vivo inflammatory response (HFO only) and production of Th2 cytokines, without changing IL-2 production from ConA-stimulated splenocytes, which may occur independent of leptin signalling. SN - 1476-5497 UR - https://www.unboundmedicine.com/medline/citation/19048014/Effects_of_feeding_fish_oil_on_mesenteric_lymph_node_cytokine_responses_in_obese_leptin_receptor_deficient_JCR:LA_cp_rats_ L2 - https://doi.org/10.1038/ijo.2008.227 DB - PRIME DP - Unbound Medicine ER -
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