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Antioxidant small molecules confer variable protection against oxidative damage in yeast mutants.
J Agric Food Chem. 2008 Dec 24; 56(24):11740-51.JA

Abstract

To assess the capacity of small molecules to function as antioxidants in pathologic conditions, a set of yeast assays utilizing strains deficient in the antioxidant machinery was applied with measurements of reactive oxygen species (ROS), glutathione (GSH/GSSG), and induction of the stress responsive proteins oye2 and oye3. Yeast strains deficient in superoxide dismutase (Delta sod1), catalase A (Delta cta1), and double-deficient in Old Yellow enzyme 2 and glutathione reductase 1 (Delta oye2 glr1) were supplemented with ascorbic acid, beta-carotene, caffeic acid, or quercetin, subjected to pro-oxidant insult, and monitored for growth recovery. Ascorbic acid and caffeic acid protected cells under most circumstances, whereas beta-carotene and quercetin protection was highly context dependent, exhibiting protection in some cases and inhibition in others. Beta-carotene and quercetin elevated substantially endogenous levels of ROS in some yeast mutants. Quercetin supplementation increased significantly GSH and GSSG levels but could not maintain GSH levels in H(2)O(2)-exposed cells. Induction of the stress response machinery was manifested by the strong up-regulation of a chromosomally encoded OYE2-GFP fusion. In the case of quercetin, there was simultaneous induction of OYE3-GFP, which was previously shown to sensitize cells to H(2)O(2)-induced programmed cell death (PCD). Taken together, the results show that mutations in the antioxidant machinery affect significantly the capacity of dietary antioxidants to protect cells.

Authors+Show Affiliations

Department of Natural Products, Mediterranean Agronomic Institute of Chania, P.O. Box 85, Chania 73100, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19049288

Citation

Amari, Foued, et al. "Antioxidant Small Molecules Confer Variable Protection Against Oxidative Damage in Yeast Mutants." Journal of Agricultural and Food Chemistry, vol. 56, no. 24, 2008, pp. 11740-51.
Amari F, Fettouche A, Samra MA, et al. Antioxidant small molecules confer variable protection against oxidative damage in yeast mutants. J Agric Food Chem. 2008;56(24):11740-51.
Amari, F., Fettouche, A., Samra, M. A., Kefalas, P., Kampranis, S. C., & Makris, A. M. (2008). Antioxidant small molecules confer variable protection against oxidative damage in yeast mutants. Journal of Agricultural and Food Chemistry, 56(24), 11740-51. https://doi.org/10.1021/jf802829r
Amari F, et al. Antioxidant Small Molecules Confer Variable Protection Against Oxidative Damage in Yeast Mutants. J Agric Food Chem. 2008 Dec 24;56(24):11740-51. PubMed PMID: 19049288.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant small molecules confer variable protection against oxidative damage in yeast mutants. AU - Amari,Foued, AU - Fettouche,Abdelmadjid, AU - Samra,Mario Abou, AU - Kefalas,Panagiotis, AU - Kampranis,Sotirios C, AU - Makris,Antonios M, PY - 2008/12/4/pubmed PY - 2009/1/27/medline PY - 2008/12/4/entrez SP - 11740 EP - 51 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 56 IS - 24 N2 - To assess the capacity of small molecules to function as antioxidants in pathologic conditions, a set of yeast assays utilizing strains deficient in the antioxidant machinery was applied with measurements of reactive oxygen species (ROS), glutathione (GSH/GSSG), and induction of the stress responsive proteins oye2 and oye3. Yeast strains deficient in superoxide dismutase (Delta sod1), catalase A (Delta cta1), and double-deficient in Old Yellow enzyme 2 and glutathione reductase 1 (Delta oye2 glr1) were supplemented with ascorbic acid, beta-carotene, caffeic acid, or quercetin, subjected to pro-oxidant insult, and monitored for growth recovery. Ascorbic acid and caffeic acid protected cells under most circumstances, whereas beta-carotene and quercetin protection was highly context dependent, exhibiting protection in some cases and inhibition in others. Beta-carotene and quercetin elevated substantially endogenous levels of ROS in some yeast mutants. Quercetin supplementation increased significantly GSH and GSSG levels but could not maintain GSH levels in H(2)O(2)-exposed cells. Induction of the stress response machinery was manifested by the strong up-regulation of a chromosomally encoded OYE2-GFP fusion. In the case of quercetin, there was simultaneous induction of OYE3-GFP, which was previously shown to sensitize cells to H(2)O(2)-induced programmed cell death (PCD). Taken together, the results show that mutations in the antioxidant machinery affect significantly the capacity of dietary antioxidants to protect cells. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19049288/Antioxidant_small_molecules_confer_variable_protection_against_oxidative_damage_in_yeast_mutants_ L2 - https://doi.org/10.1021/jf802829r DB - PRIME DP - Unbound Medicine ER -