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Pancreas beta-cells morphology, liver antioxidant enzymes and liver oxidative parameters in alloxan-resistant and alloxan-susceptible Wistar rats: a viable model system for the study of concepts into reactive oxygen species.
Fundam Clin Pharmacol. 2008 Dec; 22(6):657-66.FC

Abstract

The aim of this study was to investigate biochemical and antioxidant parameters in alloxan-resistant (ALR) and alloxan-susceptible (ALS) rats. Diabetes was induced in 60-day-old male Wistar rats by a single intraperitonial injection of alloxan (AL, 150 mg/kg). Ten days after induction, a group of rats showed a significant decrease in glycemia. This group was named alloxan-resistant group. Susceptible rats showed a remarkable increase in the plasma lipid content, blood glucose and HbA1. Glycogen content in the liver decreased significantly in the ALS group (2.08 +/- 0.41 mg%) compared with ALR group (4.22 +/- 0.18). Aspartate aminotransferase and alanine aminotransferase activities were quantified in the plasma. Interestingly, ALR rats showed a decrease in both activities (42.1 +/- 6.11 and 21.7 +/- 5.54 U/mL) when compared with ALS rats (59.1 +/- 6.55 and 58.1 +/- 7.28 U/mL). The TBARS index was significantly increased in the ALS liver (0.38 +/- 0.08 nm/mg protein) when compared with the ALR liver (0.18 +/- 0.04). Superoxide dismutase and catalase activities in the ALR (230 +/- 13 and 131 +/- 15 U/mg protein) liver showed a marked increase when compared with the ALS liver (148 +/- 13 and 68 +/- 5 U/mg protein). The immunohistochemical and hematoxilin-eosin analysis also revealed that pancreatic islets of ALR rats display a different morphology amongst the groups. These results suggest an increased regenerative or recovery process in the ALR rat pancreatic islets and an increased hepatic antioxidant defenses in these group of alloxan-resistant rats.

Authors+Show Affiliations

Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. guibehr@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19049670

Citation

Behr, Guilherme Antônio, et al. "Pancreas Beta-cells Morphology, Liver Antioxidant Enzymes and Liver Oxidative Parameters in Alloxan-resistant and Alloxan-susceptible Wistar Rats: a Viable Model System for the Study of Concepts Into Reactive Oxygen Species." Fundamental & Clinical Pharmacology, vol. 22, no. 6, 2008, pp. 657-66.
Behr GA, da Silva EG, Ferreira AR, et al. Pancreas beta-cells morphology, liver antioxidant enzymes and liver oxidative parameters in alloxan-resistant and alloxan-susceptible Wistar rats: a viable model system for the study of concepts into reactive oxygen species. Fundam Clin Pharmacol. 2008;22(6):657-66.
Behr, G. A., da Silva, E. G., Ferreira, A. R., Cerski, C. T., Dal-Pizzol, F., & Moreira, J. C. (2008). Pancreas beta-cells morphology, liver antioxidant enzymes and liver oxidative parameters in alloxan-resistant and alloxan-susceptible Wistar rats: a viable model system for the study of concepts into reactive oxygen species. Fundamental & Clinical Pharmacology, 22(6), 657-66. https://doi.org/10.1111/j.1472-8206.2008.00628.x
Behr GA, et al. Pancreas Beta-cells Morphology, Liver Antioxidant Enzymes and Liver Oxidative Parameters in Alloxan-resistant and Alloxan-susceptible Wistar Rats: a Viable Model System for the Study of Concepts Into Reactive Oxygen Species. Fundam Clin Pharmacol. 2008;22(6):657-66. PubMed PMID: 19049670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pancreas beta-cells morphology, liver antioxidant enzymes and liver oxidative parameters in alloxan-resistant and alloxan-susceptible Wistar rats: a viable model system for the study of concepts into reactive oxygen species. AU - Behr,Guilherme Antônio, AU - da Silva,Evandro Gomes, AU - Ferreira,Amâncio Romanelli, AU - Cerski,Carlos Thadeu Schmidt, AU - Dal-Pizzol,Felipe, AU - Moreira,José Cláudio Fonseca, PY - 2008/12/4/pubmed PY - 2009/2/20/medline PY - 2008/12/4/entrez SP - 657 EP - 66 JF - Fundamental & clinical pharmacology JO - Fundam Clin Pharmacol VL - 22 IS - 6 N2 - The aim of this study was to investigate biochemical and antioxidant parameters in alloxan-resistant (ALR) and alloxan-susceptible (ALS) rats. Diabetes was induced in 60-day-old male Wistar rats by a single intraperitonial injection of alloxan (AL, 150 mg/kg). Ten days after induction, a group of rats showed a significant decrease in glycemia. This group was named alloxan-resistant group. Susceptible rats showed a remarkable increase in the plasma lipid content, blood glucose and HbA1. Glycogen content in the liver decreased significantly in the ALS group (2.08 +/- 0.41 mg%) compared with ALR group (4.22 +/- 0.18). Aspartate aminotransferase and alanine aminotransferase activities were quantified in the plasma. Interestingly, ALR rats showed a decrease in both activities (42.1 +/- 6.11 and 21.7 +/- 5.54 U/mL) when compared with ALS rats (59.1 +/- 6.55 and 58.1 +/- 7.28 U/mL). The TBARS index was significantly increased in the ALS liver (0.38 +/- 0.08 nm/mg protein) when compared with the ALR liver (0.18 +/- 0.04). Superoxide dismutase and catalase activities in the ALR (230 +/- 13 and 131 +/- 15 U/mg protein) liver showed a marked increase when compared with the ALS liver (148 +/- 13 and 68 +/- 5 U/mg protein). The immunohistochemical and hematoxilin-eosin analysis also revealed that pancreatic islets of ALR rats display a different morphology amongst the groups. These results suggest an increased regenerative or recovery process in the ALR rat pancreatic islets and an increased hepatic antioxidant defenses in these group of alloxan-resistant rats. SN - 1472-8206 UR - https://www.unboundmedicine.com/medline/citation/19049670/Pancreas_beta_cells_morphology_liver_antioxidant_enzymes_and_liver_oxidative_parameters_in_alloxan_resistant_and_alloxan_susceptible_Wistar_rats:_a_viable_model_system_for_the_study_of_concepts_into_reactive_oxygen_species_ L2 - https://doi.org/10.1111/j.1472-8206.2008.00628.x DB - PRIME DP - Unbound Medicine ER -