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Local injection of BDNF producing mesenchymal stem cells increases neuronal survival and synaptic stability following ventral root avulsion.
Neurobiol Dis. 2009 Feb; 33(2):290-300.ND

Abstract

The present study proposed to graft mesenchymal stem cells (MSCs), which continuously produce BDNF, into the spinal cord ventral horn, after ventral root avulsion. Neurotrophin expression was naturally achieved by culturing MSCs in an undifferentiated state for at least 10 weeks. Lewis rats were subjected to unilateral avulsion of lumbar ventral roots, receiving 3 x 10(5) cells injected through the lateral funiculus. Two weeks after surgery, the animals were sacrificed and neuronal survival, astroglial reaction and synaptic inputs within the motor nucleus analyzed. The results indicated that the MSCs treatment significantly rescued avulsed motoneurons. Such neuronal survival was related to in vivo mRNA up regulation as well as expression of BDNF and GDNF. Such increase was correlated to the preservation of synaptophysin- positive nerve terminals. Thus it was proposed that when maintained undifferentiated for a period of 10 weeks, MSCs may be used as a continuous source of BDNF, positively influencing neuronal survival and synaptic plasticity.

Authors+Show Affiliations

Department of Biochemistry and Immunology, ICB-UFMG, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19049875

Citation

Rodrigues Hell, Rafaela Chitarra, et al. "Local Injection of BDNF Producing Mesenchymal Stem Cells Increases Neuronal Survival and Synaptic Stability Following Ventral Root Avulsion." Neurobiology of Disease, vol. 33, no. 2, 2009, pp. 290-300.
Rodrigues Hell RC, Silva Costa MM, Goes AM, et al. Local injection of BDNF producing mesenchymal stem cells increases neuronal survival and synaptic stability following ventral root avulsion. Neurobiol Dis. 2009;33(2):290-300.
Rodrigues Hell, R. C., Silva Costa, M. M., Goes, A. M., & Oliveira, A. L. (2009). Local injection of BDNF producing mesenchymal stem cells increases neuronal survival and synaptic stability following ventral root avulsion. Neurobiology of Disease, 33(2), 290-300. https://doi.org/10.1016/j.nbd.2008.10.017
Rodrigues Hell RC, et al. Local Injection of BDNF Producing Mesenchymal Stem Cells Increases Neuronal Survival and Synaptic Stability Following Ventral Root Avulsion. Neurobiol Dis. 2009;33(2):290-300. PubMed PMID: 19049875.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Local injection of BDNF producing mesenchymal stem cells increases neuronal survival and synaptic stability following ventral root avulsion. AU - Rodrigues Hell,Rafaela Chitarra, AU - Silva Costa,Miriam Maria, AU - Goes,Alfredo Miranda, AU - Oliveira,Alexandre L R, Y1 - 2008/11/12/ PY - 2008/04/16/received PY - 2008/10/20/revised PY - 2008/10/25/accepted PY - 2008/12/4/pubmed PY - 2009/3/10/medline PY - 2008/12/4/entrez SP - 290 EP - 300 JF - Neurobiology of disease JO - Neurobiol. Dis. VL - 33 IS - 2 N2 - The present study proposed to graft mesenchymal stem cells (MSCs), which continuously produce BDNF, into the spinal cord ventral horn, after ventral root avulsion. Neurotrophin expression was naturally achieved by culturing MSCs in an undifferentiated state for at least 10 weeks. Lewis rats were subjected to unilateral avulsion of lumbar ventral roots, receiving 3 x 10(5) cells injected through the lateral funiculus. Two weeks after surgery, the animals were sacrificed and neuronal survival, astroglial reaction and synaptic inputs within the motor nucleus analyzed. The results indicated that the MSCs treatment significantly rescued avulsed motoneurons. Such neuronal survival was related to in vivo mRNA up regulation as well as expression of BDNF and GDNF. Such increase was correlated to the preservation of synaptophysin- positive nerve terminals. Thus it was proposed that when maintained undifferentiated for a period of 10 weeks, MSCs may be used as a continuous source of BDNF, positively influencing neuronal survival and synaptic plasticity. SN - 1095-953X UR - https://www.unboundmedicine.com/medline/citation/19049875/Local_injection_of_BDNF_producing_mesenchymal_stem_cells_increases_neuronal_survival_and_synaptic_stability_following_ventral_root_avulsion_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0969-9961(08)00266-0 DB - PRIME DP - Unbound Medicine ER -