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Increasing erucic acid content through combination of endogenous low polyunsaturated fatty acids alleles with Ld-LPAAT + Bn-fae1 transgenes in rapeseed (Brassica napus L.).
Theor Appl Genet. 2009 Feb; 118(4):765-73.TA

Abstract

High erucic acid rapeseed (HEAR) oil is of interest for industrial purposes because erucic acid (22:1) and its derivatives are important renewable raw materials for the oleochemical industry. Currently available cultivars contain only about 50% erucic acid in the seed oil. A substantial increase in erucic acid content would significantly reduce processing costs and could increase market prospects of HEAR oil. It has been proposed that erucic acid content in rapeseed is limited because of insufficient fatty acid elongation, lack of insertion of erucic acid into the central sn-2 position of the triaclyglycerol backbone and due to competitive desaturation of the precursor oleic acid (18:1) to linoleic acid (18:2). The objective of the present study was to increase erucic content of HEAR winter rapeseed through over expression of the rapeseed fatty acid elongase gene (fae1) in combination with expression of the lysophosphatidic acid acyltransferase gene from Limnanthes douglasii (Ld-LPAAT), which enables insertion of erucic acid into the sn-2 glycerol position. Furthermore, mutant alleles for low contents of polyunsaturated fatty acids (18:2 + 18:3) were combined with the transgenic material. Selected transgenic lines showed up to 63% erucic acid in the seed oil in comparison to a mean of 54% erucic acid of segregating non-transgenic HEAR plants. Amongst 220 F(2) plants derived from the cross between a transgenic HEAR line and a non-transgenic HEAR line with a low content of polyunsaturated fatty acids, recombinant F(2) plants were identified with an erucic acid content of up to 72% and a polyunsaturated fatty acid content as low as 6%. Regression analysis revealed that a reduction of 10% in polyunsaturated fatty acids content led to a 6.5% increase in erucic acid content. Results from selected F(2) plants were confirmed in the next generation by analysing F(4) seeds harvested from five F(3) plants per selected F(2) plant. F(3) lines contained up to 72% erucic acid and as little as 4% polyunsaturated fatty acids content in the seed oil. The 72% erucic acid content of rapeseed oil achieved in the present study represents a major breakthrough in breeding high erucic acid rapeseed.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Nath UK
Department of Crop Sciences, Plant Breeding, Georg-August-Universität Göttingen, Von-Siebold-Str. 8, 37075 Göttingen, Germany.
Wilmer JA
No affiliation info available
Wallington EJ
No affiliation info available
Becker HC
No affiliation info available
Möllers C
No affiliation info available

MeSH

AcetyltransferasesAcyltransferasesAgrobacterium tumefaciensBrassica napusCrosses, GeneticDNA PrimersErucic AcidsFatty Acid ElongasesFatty Acids, UnsaturatedRegression AnalysisTransformation, GeneticTransgenes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19050848

Citation

Nath, Ujjal K., et al. "Increasing Erucic Acid Content Through Combination of Endogenous Low Polyunsaturated Fatty Acids Alleles With Ld-LPAAT + Bn-fae1 Transgenes in Rapeseed (Brassica Napus L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 118, no. 4, 2009, pp. 765-73.
Nath UK, Wilmer JA, Wallington EJ, et al. Increasing erucic acid content through combination of endogenous low polyunsaturated fatty acids alleles with Ld-LPAAT + Bn-fae1 transgenes in rapeseed (Brassica napus L.). Theor Appl Genet. 2009;118(4):765-73.
Nath, U. K., Wilmer, J. A., Wallington, E. J., Becker, H. C., & Möllers, C. (2009). Increasing erucic acid content through combination of endogenous low polyunsaturated fatty acids alleles with Ld-LPAAT + Bn-fae1 transgenes in rapeseed (Brassica napus L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 118(4), 765-73. https://doi.org/10.1007/s00122-008-0936-7
Nath UK, et al. Increasing Erucic Acid Content Through Combination of Endogenous Low Polyunsaturated Fatty Acids Alleles With Ld-LPAAT + Bn-fae1 Transgenes in Rapeseed (Brassica Napus L.). Theor Appl Genet. 2009;118(4):765-73. PubMed PMID: 19050848.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increasing erucic acid content through combination of endogenous low polyunsaturated fatty acids alleles with Ld-LPAAT + Bn-fae1 transgenes in rapeseed (Brassica napus L.). AU - Nath,Ujjal K, AU - Wilmer,Jeroen A, AU - Wallington,Emma J, AU - Becker,Heiko C, AU - Möllers,Christian, Y1 - 2008/12/03/ PY - 2008/06/25/received PY - 2008/11/13/accepted PY - 2008/12/4/pubmed PY - 2009/6/2/medline PY - 2008/12/4/entrez SP - 765 EP - 73 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 118 IS - 4 N2 - High erucic acid rapeseed (HEAR) oil is of interest for industrial purposes because erucic acid (22:1) and its derivatives are important renewable raw materials for the oleochemical industry. Currently available cultivars contain only about 50% erucic acid in the seed oil. A substantial increase in erucic acid content would significantly reduce processing costs and could increase market prospects of HEAR oil. It has been proposed that erucic acid content in rapeseed is limited because of insufficient fatty acid elongation, lack of insertion of erucic acid into the central sn-2 position of the triaclyglycerol backbone and due to competitive desaturation of the precursor oleic acid (18:1) to linoleic acid (18:2). The objective of the present study was to increase erucic content of HEAR winter rapeseed through over expression of the rapeseed fatty acid elongase gene (fae1) in combination with expression of the lysophosphatidic acid acyltransferase gene from Limnanthes douglasii (Ld-LPAAT), which enables insertion of erucic acid into the sn-2 glycerol position. Furthermore, mutant alleles for low contents of polyunsaturated fatty acids (18:2 + 18:3) were combined with the transgenic material. Selected transgenic lines showed up to 63% erucic acid in the seed oil in comparison to a mean of 54% erucic acid of segregating non-transgenic HEAR plants. Amongst 220 F(2) plants derived from the cross between a transgenic HEAR line and a non-transgenic HEAR line with a low content of polyunsaturated fatty acids, recombinant F(2) plants were identified with an erucic acid content of up to 72% and a polyunsaturated fatty acid content as low as 6%. Regression analysis revealed that a reduction of 10% in polyunsaturated fatty acids content led to a 6.5% increase in erucic acid content. Results from selected F(2) plants were confirmed in the next generation by analysing F(4) seeds harvested from five F(3) plants per selected F(2) plant. F(3) lines contained up to 72% erucic acid and as little as 4% polyunsaturated fatty acids content in the seed oil. The 72% erucic acid content of rapeseed oil achieved in the present study represents a major breakthrough in breeding high erucic acid rapeseed. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/19050848/Increasing_erucic_acid_content_through_combination_of_endogenous_low_polyunsaturated_fatty_acids_alleles_with_Ld_LPAAT_+_Bn_fae1_transgenes_in_rapeseed__Brassica_napus_L___ L2 - https://dx.doi.org/10.1007/s00122-008-0936-7 DB - PRIME DP - Unbound Medicine ER -