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Time-dependent protective efficacy of Trolox (vitamin E analog) against microcystin-induced toxicity in tilapia (Oreochromis niloticus).
Environ Toxicol. 2009 Dec; 24(6):563-79.ET

Abstract

Microcystins (MCs), hepatotoxins from cyanobacteria, induce oxidative stress and pathological changes in fish that can be ameliorated with chemoprotectants such as vitamin E (vit E). This study investigated the time period after MCs exposure in which Trolox, a vitamin E analog, is effective against oxidative and histological damage in different organs of tilapia (Oreochromis niloticus). Fish were fed Trolox supplement (700 mg/kg diet) for 7 days, or received only commercial fish food, and then were exposed to a single oral dose of 120 microg/fish microcystin-LR, and sacrificed in 24, 48, or 72 h. The Trolox protective efficacy was evaluated based on lipid peroxidation (LPO), protein oxidation, enzymatic and non-enzymatic antioxidants, and a morphologic study. Regarding the oxidative stress biomarkers altered by MCs, the higher protective action of Trolox was observed 24 h post toxin exposure, although it extends also until 48 h in gills (superoxide dismutase (SOD), catalase (CAT)), and liver, where glutathione reductase (GR) backed to control values 48 and 72 h after the toxin application. Glutathione-S- transferase (GST) activity in the liver was ameliorated by the chemoprotectant after 24 and 48 h, although control values were not recovered. Trolox modulation of these biomarkers and its ability to quench free radicals explain the recovery of LPO values in all organs at 24 h and also in gills at 48 h. Histopathologically, Trolox efficacy was more evident after 72 h.

Authors+Show Affiliations

Area of Toxicology, Faculty of Pharmacy, University of Seville, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19051281

Citation

Prieto, Ana Isabel, et al. "Time-dependent Protective Efficacy of Trolox (vitamin E Analog) Against Microcystin-induced Toxicity in Tilapia (Oreochromis Niloticus)." Environmental Toxicology, vol. 24, no. 6, 2009, pp. 563-79.
Prieto AI, Jos A, Pichardo S, et al. Time-dependent protective efficacy of Trolox (vitamin E analog) against microcystin-induced toxicity in tilapia (Oreochromis niloticus). Environ Toxicol. 2009;24(6):563-79.
Prieto, A. I., Jos, A., Pichardo, S., Moreno, I., de Sotomayor, M. A., Moyano, R., Blanco, A., & Cameán, A. M. (2009). Time-dependent protective efficacy of Trolox (vitamin E analog) against microcystin-induced toxicity in tilapia (Oreochromis niloticus). Environmental Toxicology, 24(6), 563-79. https://doi.org/10.1002/tox.20458
Prieto AI, et al. Time-dependent Protective Efficacy of Trolox (vitamin E Analog) Against Microcystin-induced Toxicity in Tilapia (Oreochromis Niloticus). Environ Toxicol. 2009;24(6):563-79. PubMed PMID: 19051281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Time-dependent protective efficacy of Trolox (vitamin E analog) against microcystin-induced toxicity in tilapia (Oreochromis niloticus). AU - Prieto,Ana Isabel, AU - Jos,Angeles, AU - Pichardo,Silvia, AU - Moreno,Isabel, AU - de Sotomayor,María Alvarez, AU - Moyano,Rosario, AU - Blanco,Alfonso, AU - Cameán,Ana María, PY - 2008/12/4/pubmed PY - 2010/1/8/medline PY - 2008/12/4/entrez SP - 563 EP - 79 JF - Environmental toxicology JO - Environ Toxicol VL - 24 IS - 6 N2 - Microcystins (MCs), hepatotoxins from cyanobacteria, induce oxidative stress and pathological changes in fish that can be ameliorated with chemoprotectants such as vitamin E (vit E). This study investigated the time period after MCs exposure in which Trolox, a vitamin E analog, is effective against oxidative and histological damage in different organs of tilapia (Oreochromis niloticus). Fish were fed Trolox supplement (700 mg/kg diet) for 7 days, or received only commercial fish food, and then were exposed to a single oral dose of 120 microg/fish microcystin-LR, and sacrificed in 24, 48, or 72 h. The Trolox protective efficacy was evaluated based on lipid peroxidation (LPO), protein oxidation, enzymatic and non-enzymatic antioxidants, and a morphologic study. Regarding the oxidative stress biomarkers altered by MCs, the higher protective action of Trolox was observed 24 h post toxin exposure, although it extends also until 48 h in gills (superoxide dismutase (SOD), catalase (CAT)), and liver, where glutathione reductase (GR) backed to control values 48 and 72 h after the toxin application. Glutathione-S- transferase (GST) activity in the liver was ameliorated by the chemoprotectant after 24 and 48 h, although control values were not recovered. Trolox modulation of these biomarkers and its ability to quench free radicals explain the recovery of LPO values in all organs at 24 h and also in gills at 48 h. Histopathologically, Trolox efficacy was more evident after 72 h. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/19051281/Time_dependent_protective_efficacy_of_Trolox__vitamin_E_analog__against_microcystin_induced_toxicity_in_tilapia__Oreochromis_niloticus__ L2 - https://doi.org/10.1002/tox.20458 DB - PRIME DP - Unbound Medicine ER -