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Effects of amburoside A and isokaempferide, polyphenols from Amburana cearensis, on rodent inflammatory processes and myeloperoxidase activity in human neutrophils.
Basic Clin Pharmacol Toxicol. 2009 Mar; 104(3):198-205.BC

Abstract

The present study evaluated the anti-inflammatory activity of amburoside A (a phenol glucoside) and isokaempferide (a flavonol) isolated from the trunk bark of Amburana cearensis, a medicinal plant used in northeast Brazil for the treatment of asthma. Animals (male Wistar rats or Swiss mice) pre-treated with amburoside A (25 and 50 mg/kg) or isokaempferide (12.5, 25 and 50 mg/kg), orally or intraperitoneally, showed a significant inhibition of the paw oedema induced by carrageenan (1%), prostaglandin E(2) (30 nmol/paw), histamine (200 microg/paw) or serotonin (200 microg/paw). Histological and morphometric evaluations of the rat paw oedema induced by carrageenan showed that amburoside A and isokaempferide also inhibited the accumulation of inflammatory cells. Amburoside A reduced significantly the paw oedema and the increase in vascular permeability induced by dextran, as related to the control group. Similar results were observed with the isokaempferide pre-treatment. Furthermore, amburoside A or isokaempferide inhibited both leucocyte and neutrophil migrations, in mouse peritoneal cavity, after the carrageenan injection. The polyphenols were not cytotoxic and blocked N-formyl-methyl-leucyl-phenylalanine-induced myeloperoxidase release and activity in human neutrophils. In addition, amburoside A and isokaempferide at 50 and 100 microg/ml concentrations reduced significantly the lipopolysaccharide-mediated increase in tumour necrosis factor-alpha (TNF-alpha) levels. These results provide, for the first time, evidence to support the anti-inflammatory activity of amburoside A and isokaempferide that seems to be related to an inhibition of inflammatory mediators, such as TNF-alpha, as well as histamine, serotonin and prostaglandin E(2), besides leucocyte infiltration in a dose- or concentration-dependent manner. These anti-inflammatory effects can be explained, at least in part, by the ability of these compounds to reduce neutrophil degranulation, myeloperoxidase activity, mediators as well as TNF-alpha secretion.

Authors+Show Affiliations

Department of Pharmacy, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Brazil. kalyne@ufc.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19053991

Citation

Leal, Luzia Kalyne Almeida Moreira, et al. "Effects of Amburoside a and Isokaempferide, Polyphenols From Amburana Cearensis, On Rodent Inflammatory Processes and Myeloperoxidase Activity in Human Neutrophils." Basic & Clinical Pharmacology & Toxicology, vol. 104, no. 3, 2009, pp. 198-205.
Leal LK, Canuto KM, da Silva Costa KC, et al. Effects of amburoside A and isokaempferide, polyphenols from Amburana cearensis, on rodent inflammatory processes and myeloperoxidase activity in human neutrophils. Basic Clin Pharmacol Toxicol. 2009;104(3):198-205.
Leal, L. K., Canuto, K. M., da Silva Costa, K. C., Nobre-Júnior, H. V., Vasconcelos, S. M., Silveira, E. R., Ferreira, M. V., Fontenele, J. B., Andrade, G. M., & de Barros Viana, G. S. (2009). Effects of amburoside A and isokaempferide, polyphenols from Amburana cearensis, on rodent inflammatory processes and myeloperoxidase activity in human neutrophils. Basic & Clinical Pharmacology & Toxicology, 104(3), 198-205. https://doi.org/10.1111/j.1742-7843.2008.00329.x
Leal LK, et al. Effects of Amburoside a and Isokaempferide, Polyphenols From Amburana Cearensis, On Rodent Inflammatory Processes and Myeloperoxidase Activity in Human Neutrophils. Basic Clin Pharmacol Toxicol. 2009;104(3):198-205. PubMed PMID: 19053991.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of amburoside A and isokaempferide, polyphenols from Amburana cearensis, on rodent inflammatory processes and myeloperoxidase activity in human neutrophils. AU - Leal,Luzia Kalyne Almeida Moreira, AU - Canuto,Kirley Marques, AU - da Silva Costa,Kassiane Cristine, AU - Nobre-Júnior,Hélio Vitoriano, AU - Vasconcelos,Silvânia Mendes, AU - Silveira,Edilberto Rocha, AU - Ferreira,Márcia Valéria Pitombeira, AU - Fontenele,Juvênia Bezerra, AU - Andrade,Geane Matos, AU - de Barros Viana,Glauce Socorro, Y1 - 2008/11/14/ PY - 2008/12/5/pubmed PY - 2009/4/11/medline PY - 2008/12/5/entrez SP - 198 EP - 205 JF - Basic & clinical pharmacology & toxicology JO - Basic Clin Pharmacol Toxicol VL - 104 IS - 3 N2 - The present study evaluated the anti-inflammatory activity of amburoside A (a phenol glucoside) and isokaempferide (a flavonol) isolated from the trunk bark of Amburana cearensis, a medicinal plant used in northeast Brazil for the treatment of asthma. Animals (male Wistar rats or Swiss mice) pre-treated with amburoside A (25 and 50 mg/kg) or isokaempferide (12.5, 25 and 50 mg/kg), orally or intraperitoneally, showed a significant inhibition of the paw oedema induced by carrageenan (1%), prostaglandin E(2) (30 nmol/paw), histamine (200 microg/paw) or serotonin (200 microg/paw). Histological and morphometric evaluations of the rat paw oedema induced by carrageenan showed that amburoside A and isokaempferide also inhibited the accumulation of inflammatory cells. Amburoside A reduced significantly the paw oedema and the increase in vascular permeability induced by dextran, as related to the control group. Similar results were observed with the isokaempferide pre-treatment. Furthermore, amburoside A or isokaempferide inhibited both leucocyte and neutrophil migrations, in mouse peritoneal cavity, after the carrageenan injection. The polyphenols were not cytotoxic and blocked N-formyl-methyl-leucyl-phenylalanine-induced myeloperoxidase release and activity in human neutrophils. In addition, amburoside A and isokaempferide at 50 and 100 microg/ml concentrations reduced significantly the lipopolysaccharide-mediated increase in tumour necrosis factor-alpha (TNF-alpha) levels. These results provide, for the first time, evidence to support the anti-inflammatory activity of amburoside A and isokaempferide that seems to be related to an inhibition of inflammatory mediators, such as TNF-alpha, as well as histamine, serotonin and prostaglandin E(2), besides leucocyte infiltration in a dose- or concentration-dependent manner. These anti-inflammatory effects can be explained, at least in part, by the ability of these compounds to reduce neutrophil degranulation, myeloperoxidase activity, mediators as well as TNF-alpha secretion. SN - 1742-7843 UR - https://www.unboundmedicine.com/medline/citation/19053991/Effects_of_amburoside_A_and_isokaempferide_polyphenols_from_Amburana_cearensis_on_rodent_inflammatory_processes_and_myeloperoxidase_activity_in_human_neutrophils_ L2 - https://doi.org/10.1111/j.1742-7843.2008.00329.x DB - PRIME DP - Unbound Medicine ER -