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Vacuolar dynamics during the morphogenetic transition in Candida albicans.

Abstract

Morphogenetic transitions between the yeast, pseudohyphal and true hyphal forms of the fungal pathogen Candida albicans are coordinated with the cell cycle. In true hyphae, large vacuoles are formed in subapical compartments that influence compartment size and hence cell cycle progression and branching frequency. Here, live-cell imaging techniques were applied to study the dynamic process of vacuolar segregation in the three different morphological forms of C. albicans. We show the presence of a filamentous vacuole segregation structure in pseudohyphae and true hyphae that differs from that of a stream of vacuole vesicles typical of vacuole inheritance in yeast cells. In growing hyphae, retrograde transport of the vacuoles led to apparent capture and adhesion of vacuoles to supapical septa, and septation events sometimes led to the bisection of large vacuoles that occupied sites of cytokinesis. Inhibitor studies also demonstrated that vacuole motility in C. albicans was actin dependent and microtubule independent.

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  • Authors+Show Affiliations

    ,

    The Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK.

    Source

    FEMS yeast research 8:8 2008 Dec pg 1339-48

    MeSH

    Actins
    Candida albicans
    Hyphae
    Image Processing, Computer-Assisted
    Microscopy, Fluorescence
    Microscopy, Interference
    Microtubules
    Morphogenesis
    Vacuoles

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    19054134

    Citation

    TY - JOUR T1 - Vacuolar dynamics during the morphogenetic transition in Candida albicans. AU - Veses,Verónica, AU - Gow,Neil A R, PY - 2008/12/5/pubmed PY - 2009/1/13/medline PY - 2008/12/5/entrez SP - 1339 EP - 48 JF - FEMS yeast research JO - FEMS Yeast Res. VL - 8 IS - 8 N2 - Morphogenetic transitions between the yeast, pseudohyphal and true hyphal forms of the fungal pathogen Candida albicans are coordinated with the cell cycle. In true hyphae, large vacuoles are formed in subapical compartments that influence compartment size and hence cell cycle progression and branching frequency. Here, live-cell imaging techniques were applied to study the dynamic process of vacuolar segregation in the three different morphological forms of C. albicans. We show the presence of a filamentous vacuole segregation structure in pseudohyphae and true hyphae that differs from that of a stream of vacuole vesicles typical of vacuole inheritance in yeast cells. In growing hyphae, retrograde transport of the vacuoles led to apparent capture and adhesion of vacuoles to supapical septa, and septation events sometimes led to the bisection of large vacuoles that occupied sites of cytokinesis. Inhibitor studies also demonstrated that vacuole motility in C. albicans was actin dependent and microtubule independent. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/19054134/Vacuolar_dynamics_during_the_morphogenetic_transition_in_Candida_albicans_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1111/j.1567-1364.2008.00447.x ER -