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Protective effect of sulfhydryl-containing antioxidants against ischemia/reperfusion injury of prepubertal rat intestine.
J Gastroenterol Hepatol. 2009 Apr; 24(4):681-7.JG

Abstract

BACKGROUND AND AIM

Reactive oxygen species generated during reperfusion of the tissue are known to play an important role in the basic pathophysiology of ischemia/reperfusion (I/R) injury. The aim of this study was to investigate and compare the protective effects of three sulfide-based antioxidants, N-acetylcysteine (NAC), erdosteine (ERD), and alpha-lipoic acid (LA), on I/R injury of the small intestine tissue.

METHODS

Forty male Sprague-Dawley rats weighing between 100-150 g were divided into five groups (n = 8 for each): control (sham operated), I/R, I/R + NAC, I/R + ERD, and I/R + LA. Intestinal ischemia was provided by occluding the superior mesenteric artery via a special microvascular clamp; ischemia for 30 min and reperfusion for 3 days were carried out. Ileal specimens were obtained to determine the tissue levels of malondialdehyde (MDA), protein carbonyl contents (PCO), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities and histological changes.

RESULTS

The rats subjected to intestinal I/R exhibited an increase in tissue MDA and PCO; the levels could hardly be ameliorated in the treatment groups. SOD and GPx activities were significantly decreased in the I/R group, whereas their reduction was less expressed in the treatment groups. Additionally, the histopathological injury scores of the disulfide-treated groups were lower than those of the I/R group.

CONCLUSION

All of the sulfhydryl-containing antioxidants used in this study exhibited a significant role in attenuating intestinal I/R injury; however, the outcome of the LA-treated group was significantly marked than that of the others.

Authors+Show Affiliations

Department of Pediatrics, Gülhane Military Medical Academy, Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19054265

Citation

Tunc, Turan, et al. "Protective Effect of Sulfhydryl-containing Antioxidants Against Ischemia/reperfusion Injury of Prepubertal Rat Intestine." Journal of Gastroenterology and Hepatology, vol. 24, no. 4, 2009, pp. 681-7.
Tunc T, Oter S, Güven A, et al. Protective effect of sulfhydryl-containing antioxidants against ischemia/reperfusion injury of prepubertal rat intestine. J Gastroenterol Hepatol. 2009;24(4):681-7.
Tunc, T., Oter, S., Güven, A., Topal, T., Kul, M., Korkmaz, A., Ongürü, O., & Sarici, U. (2009). Protective effect of sulfhydryl-containing antioxidants against ischemia/reperfusion injury of prepubertal rat intestine. Journal of Gastroenterology and Hepatology, 24(4), 681-7. https://doi.org/10.1111/j.1440-1746.2008.05673.x
Tunc T, et al. Protective Effect of Sulfhydryl-containing Antioxidants Against Ischemia/reperfusion Injury of Prepubertal Rat Intestine. J Gastroenterol Hepatol. 2009;24(4):681-7. PubMed PMID: 19054265.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of sulfhydryl-containing antioxidants against ischemia/reperfusion injury of prepubertal rat intestine. AU - Tunc,Turan, AU - Oter,Sükrü, AU - Güven,Ahmet, AU - Topal,Turgut, AU - Kul,Mustafa, AU - Korkmaz,Ahmet, AU - Ongürü,Onder, AU - Sarici,Umit, Y1 - 2009/12/01/ PY - 2008/12/5/pubmed PY - 2009/7/3/medline PY - 2008/12/5/entrez SP - 681 EP - 7 JF - Journal of gastroenterology and hepatology JO - J Gastroenterol Hepatol VL - 24 IS - 4 N2 - BACKGROUND AND AIM: Reactive oxygen species generated during reperfusion of the tissue are known to play an important role in the basic pathophysiology of ischemia/reperfusion (I/R) injury. The aim of this study was to investigate and compare the protective effects of three sulfide-based antioxidants, N-acetylcysteine (NAC), erdosteine (ERD), and alpha-lipoic acid (LA), on I/R injury of the small intestine tissue. METHODS: Forty male Sprague-Dawley rats weighing between 100-150 g were divided into five groups (n = 8 for each): control (sham operated), I/R, I/R + NAC, I/R + ERD, and I/R + LA. Intestinal ischemia was provided by occluding the superior mesenteric artery via a special microvascular clamp; ischemia for 30 min and reperfusion for 3 days were carried out. Ileal specimens were obtained to determine the tissue levels of malondialdehyde (MDA), protein carbonyl contents (PCO), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities and histological changes. RESULTS: The rats subjected to intestinal I/R exhibited an increase in tissue MDA and PCO; the levels could hardly be ameliorated in the treatment groups. SOD and GPx activities were significantly decreased in the I/R group, whereas their reduction was less expressed in the treatment groups. Additionally, the histopathological injury scores of the disulfide-treated groups were lower than those of the I/R group. CONCLUSION: All of the sulfhydryl-containing antioxidants used in this study exhibited a significant role in attenuating intestinal I/R injury; however, the outcome of the LA-treated group was significantly marked than that of the others. SN - 1440-1746 UR - https://www.unboundmedicine.com/medline/citation/19054265/Protective_effect_of_sulfhydryl_containing_antioxidants_against_ischemia/reperfusion_injury_of_prepubertal_rat_intestine_ L2 - https://doi.org/10.1111/j.1440-1746.2008.05673.x DB - PRIME DP - Unbound Medicine ER -