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Quantitative microleakage evaluation of fissure sealants applied with or without a bonding agent: results after four-year water storage in vitro.
J Adhes Dent. 2008 Oct; 10(5):379-84.JA

Abstract

PURPOSE

To evaluate the effect of long-term water storage on the microleakage of a fissure sealant applied with or without different bonding agents.

MATERIALS AND METHODS

Extracted human third molars were randomly assigned into 8 groups (n = 24/each). The occlusal surfaces were sealed with a fluoride fissure sealant material (Helioseal F) after one of the following pretreatments: (1) phosphoric acid etching only; (2) phosphoric acid etching + Single Bond; (3) phosphoric acid etching + Prime & Bond NT; (4) Clearfil SE Bond; (5) FL Bond; (6) One Up Bond F; (7) Prompt L-Pop; (8) Mac Bond II. All specimens were thermocycled (1000X), after which half of them (n=96) were stored in distilled water for 48 h (group A), and the remaining half (n=96) for 48 months (group B). The specimens were immersed in 0.5% basic fuchsin solution, sectioned, and digitally photographed. Microleakage was evaluated quantitatively using an open-source image analysis toolkit (ImageJ), and the data were analyzed statistically.

RESULTS

Four-year water storage significantly increased the amount of leakage in all test groups (p < 0.001). In both the absence and presence of water aging, the etch-and-rinse adhesives yielded the lowest microleakage scores (p < 0.001). In the 48-h group, the following ranking was achieved in terms microleakage values: phosphoric acid + Prime & Bond NT = phosphoric acid + Single Bond < Prompt L-Pop = phosphoric acid etching only < FL Bond < Clearfil SE Bond < Mac Bond II = One Up Bond F. In the 48-month group, the ranking changed as follows: phosphoric acid + Prime & Bond NT = phosphoric acid + Single Bond < Prompt L-Pop = FL Bond < Clearfil SE Bond = Mac Bond II = One Up Bond F < phosphoric acid etching only.

CONCLUSION

Long-term water storage significantly increased the microleakage of Helioseal F applied alone and with a bonding agent. Regardless of the storage term, the use of etch-and-rinse adhesives resulted in significantly less microleakage compared to that achieved with self-etching adhesives or acid etching alone. The sealants placed without a prior bonding agent showed the greatest amount of leakage after four years.

Authors+Show Affiliations

Department of Pediatric Dentistry, Faculty of Dentistry, Hacettepe University, Ankara, Turkey.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

19058684

Citation

Cehreli, Z C., and H C. Gungor. "Quantitative Microleakage Evaluation of Fissure Sealants Applied With or Without a Bonding Agent: Results After Four-year Water Storage in Vitro." The Journal of Adhesive Dentistry, vol. 10, no. 5, 2008, pp. 379-84.
Cehreli ZC, Gungor HC. Quantitative microleakage evaluation of fissure sealants applied with or without a bonding agent: results after four-year water storage in vitro. J Adhes Dent. 2008;10(5):379-84.
Cehreli, Z. C., & Gungor, H. C. (2008). Quantitative microleakage evaluation of fissure sealants applied with or without a bonding agent: results after four-year water storage in vitro. The Journal of Adhesive Dentistry, 10(5), 379-84.
Cehreli ZC, Gungor HC. Quantitative Microleakage Evaluation of Fissure Sealants Applied With or Without a Bonding Agent: Results After Four-year Water Storage in Vitro. J Adhes Dent. 2008;10(5):379-84. PubMed PMID: 19058684.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative microleakage evaluation of fissure sealants applied with or without a bonding agent: results after four-year water storage in vitro. AU - Cehreli,Z C, AU - Gungor,H C, PY - 2008/12/9/pubmed PY - 2009/2/12/medline PY - 2008/12/9/entrez SP - 379 EP - 84 JF - The journal of adhesive dentistry JO - J Adhes Dent VL - 10 IS - 5 N2 - PURPOSE: To evaluate the effect of long-term water storage on the microleakage of a fissure sealant applied with or without different bonding agents. MATERIALS AND METHODS: Extracted human third molars were randomly assigned into 8 groups (n = 24/each). The occlusal surfaces were sealed with a fluoride fissure sealant material (Helioseal F) after one of the following pretreatments: (1) phosphoric acid etching only; (2) phosphoric acid etching + Single Bond; (3) phosphoric acid etching + Prime & Bond NT; (4) Clearfil SE Bond; (5) FL Bond; (6) One Up Bond F; (7) Prompt L-Pop; (8) Mac Bond II. All specimens were thermocycled (1000X), after which half of them (n=96) were stored in distilled water for 48 h (group A), and the remaining half (n=96) for 48 months (group B). The specimens were immersed in 0.5% basic fuchsin solution, sectioned, and digitally photographed. Microleakage was evaluated quantitatively using an open-source image analysis toolkit (ImageJ), and the data were analyzed statistically. RESULTS: Four-year water storage significantly increased the amount of leakage in all test groups (p < 0.001). In both the absence and presence of water aging, the etch-and-rinse adhesives yielded the lowest microleakage scores (p < 0.001). In the 48-h group, the following ranking was achieved in terms microleakage values: phosphoric acid + Prime & Bond NT = phosphoric acid + Single Bond < Prompt L-Pop = phosphoric acid etching only < FL Bond < Clearfil SE Bond < Mac Bond II = One Up Bond F. In the 48-month group, the ranking changed as follows: phosphoric acid + Prime & Bond NT = phosphoric acid + Single Bond < Prompt L-Pop = FL Bond < Clearfil SE Bond = Mac Bond II = One Up Bond F < phosphoric acid etching only. CONCLUSION: Long-term water storage significantly increased the microleakage of Helioseal F applied alone and with a bonding agent. Regardless of the storage term, the use of etch-and-rinse adhesives resulted in significantly less microleakage compared to that achieved with self-etching adhesives or acid etching alone. The sealants placed without a prior bonding agent showed the greatest amount of leakage after four years. SN - 1461-5185 UR - https://www.unboundmedicine.com/medline/citation/19058684/Quantitative_microleakage_evaluation_of_fissure_sealants_applied_with_or_without_a_bonding_agent:_results_after_four_year_water_storage_in_vitro_ DB - PRIME DP - Unbound Medicine ER -