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Selective enrichment of aminothiols using polysorbate 20-capped gold nanoparticles followed by capillary electrophoresis with laser-induced fluorescence.
J Chromatogr A. 2009 Jan 09; 1216(2):288-93.JC

Abstract

In this article, we report a simple method for selective enrichment of aminothiols using Tween 20-capped gold nanoparticles (AuNPs) prior to capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF). Compared to citrate-capped AuNPs, Tween 20-capped AuNPs exhibit the ability to disperse in a highly saline solution and selectively extract aminothiols through the formation of Au-S bonds. After extraction and centrifugation, 1mM thioglycollic acid (TGA) was utilized to remove aminothiols that attached to the NP surfaces. After a solution of 8.0 mL aminothiols were extracted using 2x AuNPs (200 microL), the extracted aminothiols derivatized with o-phthalaldehyde at pH 12.0 were detected by CE-LIF. As a result, the limits of detection at a signal-to-noise ratio of 3 for homocysteine (HCys), glutathione (GSH), and gamma-glutamycysteine (Glu-cys) are 4013.2, 79.8, and 382.8 pM, respectively. The use of this probe provided approximately 11-, 282-, and 21-fold sensitivity improvements for HCys, GSH, and Glu-cys, respectively. A practical analysis of HCys, GSH, and Glu-cys in human urine sample has been accomplished by this present method.

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Authors+Show Affiliations

Shen CC
Department of Chemistry, National Sun Yat-sen University, 70, Lien-hai Road, Kaohsiung 804, Taiwan.
Tseng WL
No affiliation info available
Hsieh MM
No affiliation info available

MeSH

Amino Acids, SulfurDipeptidesElectrophoresis, CapillaryFluorescenceGlutathioneGoldHomocysteineHumansLasersLinear ModelsMetal NanoparticlesPolysorbatesReproducibility of ResultsSensitivity and Specificityo-Phthalaldehyde

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19058808

Citation

Shen, Chien-Chih, et al. "Selective Enrichment of Aminothiols Using Polysorbate 20-capped Gold Nanoparticles Followed By Capillary Electrophoresis With Laser-induced Fluorescence." Journal of Chromatography. A, vol. 1216, no. 2, 2009, pp. 288-93.
Shen CC, Tseng WL, Hsieh MM. Selective enrichment of aminothiols using polysorbate 20-capped gold nanoparticles followed by capillary electrophoresis with laser-induced fluorescence. J Chromatogr A. 2009;1216(2):288-93.
Shen, C. C., Tseng, W. L., & Hsieh, M. M. (2009). Selective enrichment of aminothiols using polysorbate 20-capped gold nanoparticles followed by capillary electrophoresis with laser-induced fluorescence. Journal of Chromatography. A, 1216(2), 288-93. https://doi.org/10.1016/j.chroma.2008.11.044
Shen CC, Tseng WL, Hsieh MM. Selective Enrichment of Aminothiols Using Polysorbate 20-capped Gold Nanoparticles Followed By Capillary Electrophoresis With Laser-induced Fluorescence. J Chromatogr A. 2009 Jan 9;1216(2):288-93. PubMed PMID: 19058808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective enrichment of aminothiols using polysorbate 20-capped gold nanoparticles followed by capillary electrophoresis with laser-induced fluorescence. AU - Shen,Chien-Chih, AU - Tseng,Wei-Lung, AU - Hsieh,Ming-Mu, Y1 - 2008/11/21/ PY - 2008/09/08/received PY - 2008/11/09/revised PY - 2008/11/17/accepted PY - 2008/12/9/pubmed PY - 2009/3/20/medline PY - 2008/12/9/entrez SP - 288 EP - 93 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 2 N2 - In this article, we report a simple method for selective enrichment of aminothiols using Tween 20-capped gold nanoparticles (AuNPs) prior to capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF). Compared to citrate-capped AuNPs, Tween 20-capped AuNPs exhibit the ability to disperse in a highly saline solution and selectively extract aminothiols through the formation of Au-S bonds. After extraction and centrifugation, 1mM thioglycollic acid (TGA) was utilized to remove aminothiols that attached to the NP surfaces. After a solution of 8.0 mL aminothiols were extracted using 2x AuNPs (200 microL), the extracted aminothiols derivatized with o-phthalaldehyde at pH 12.0 were detected by CE-LIF. As a result, the limits of detection at a signal-to-noise ratio of 3 for homocysteine (HCys), glutathione (GSH), and gamma-glutamycysteine (Glu-cys) are 4013.2, 79.8, and 382.8 pM, respectively. The use of this probe provided approximately 11-, 282-, and 21-fold sensitivity improvements for HCys, GSH, and Glu-cys, respectively. A practical analysis of HCys, GSH, and Glu-cys in human urine sample has been accomplished by this present method. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/19058808/Selective_enrichment_of_aminothiols_using_polysorbate_20_capped_gold_nanoparticles_followed_by_capillary_electrophoresis_with_laser_induced_fluorescence_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)02029-3 DB - PRIME DP - Unbound Medicine ER -