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Recombinant modified vaccinia virus Ankara expressing the hemagglutinin gene confers protection against homologous and heterologous H5N1 influenza virus infections in macaques.
J Infect Dis. 2009 Feb 01; 199(3):405-13.JI

Abstract

BACKGROUND

Highly pathogenic avian influenza viruses of the H5N1 subtype have been responsible for an increasing number of infections in humans since 2003. More than 60% of infected individuals die, and new infections are reported frequently. In light of the pandemic threat caused by these events, the rapid availability of safe and effective vaccines is desirable. Modified vaccinia virus Ankara (MVA) expressing the hemagglutinin (HA) gene of H5N1 viruses is a promising candidate vaccine that induced protective immunity against infection with homologous and heterologous H5N1 influenza virus in mice.

METHODS

In the present study, we evaluated a recombinant MVA vector expressing the HA gene of H5N1 influenza virus A/Vietnam/1194/04 (MVA-HA-VN/04) in nonhuman primates. Cynomolgus macaques were immunized twice and then were challenged with influenza virus A/Vietnam/1194/04 (clade 1) or A/Indonesia/5/05 (clade 2.1) to assess the level of protective immunity.

RESULTS

Immunization with MVA-HA-VN/04 induced (cross-reactive) antibodies and prevented virus replication in the upper and lower respiratory tract and the development of severe necrotizing bronchointerstitial pneumonia.

CONCLUSION

Therefore, MVA-HA-VN/04 is a promising vaccine candidate for the induction of protective immunity against highly pathogenic H5N1 avian influenza viruses in humans.

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Authors+Show Affiliations

Kreijtz JH
Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands.
Suezer Y
No affiliation info available
de Mutsert G
No affiliation info available
van den Brand JM
No affiliation info available
van Amerongen G
No affiliation info available
Schnierle BS
No affiliation info available
Kuiken T
No affiliation info available
Fouchier RA
No affiliation info available
Löwer J
No affiliation info available
Osterhaus AD
No affiliation info available
Sutter G
No affiliation info available
Rimmelzwaan GF
No affiliation info available

MeSH

AnimalsAntibodies, ViralBrainHemagglutininsImmunohistochemistryInfluenza A Virus, H5N1 SubtypeInfluenza VaccinesLungMacaca fascicularisOrthomyxoviridae InfectionsRecombination, GeneticRespiratory SystemSpleenVaccines, SyntheticVaccinia virus

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19061423

Citation

Kreijtz, J H C M., et al. "Recombinant Modified Vaccinia Virus Ankara Expressing the Hemagglutinin Gene Confers Protection Against Homologous and Heterologous H5N1 Influenza Virus Infections in Macaques." The Journal of Infectious Diseases, vol. 199, no. 3, 2009, pp. 405-13.
Kreijtz JH, Suezer Y, de Mutsert G, et al. Recombinant modified vaccinia virus Ankara expressing the hemagglutinin gene confers protection against homologous and heterologous H5N1 influenza virus infections in macaques. J Infect Dis. 2009;199(3):405-13.
Kreijtz, J. H., Suezer, Y., de Mutsert, G., van den Brand, J. M., van Amerongen, G., Schnierle, B. S., Kuiken, T., Fouchier, R. A., Löwer, J., Osterhaus, A. D., Sutter, G., & Rimmelzwaan, G. F. (2009). Recombinant modified vaccinia virus Ankara expressing the hemagglutinin gene confers protection against homologous and heterologous H5N1 influenza virus infections in macaques. The Journal of Infectious Diseases, 199(3), 405-13. https://doi.org/10.1086/595984
Kreijtz JH, et al. Recombinant Modified Vaccinia Virus Ankara Expressing the Hemagglutinin Gene Confers Protection Against Homologous and Heterologous H5N1 Influenza Virus Infections in Macaques. J Infect Dis. 2009 Feb 1;199(3):405-13. PubMed PMID: 19061423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recombinant modified vaccinia virus Ankara expressing the hemagglutinin gene confers protection against homologous and heterologous H5N1 influenza virus infections in macaques. AU - Kreijtz,J H C M, AU - Suezer,Y, AU - de Mutsert,G, AU - van den Brand,J M A, AU - van Amerongen,G, AU - Schnierle,B S, AU - Kuiken,T, AU - Fouchier,R A M, AU - Löwer,J, AU - Osterhaus,A D M E, AU - Sutter,G, AU - Rimmelzwaan,G F, PY - 2008/12/9/pubmed PY - 2009/3/13/medline PY - 2008/12/9/entrez SP - 405 EP - 13 JF - The Journal of infectious diseases JO - J Infect Dis VL - 199 IS - 3 N2 - BACKGROUND: Highly pathogenic avian influenza viruses of the H5N1 subtype have been responsible for an increasing number of infections in humans since 2003. More than 60% of infected individuals die, and new infections are reported frequently. In light of the pandemic threat caused by these events, the rapid availability of safe and effective vaccines is desirable. Modified vaccinia virus Ankara (MVA) expressing the hemagglutinin (HA) gene of H5N1 viruses is a promising candidate vaccine that induced protective immunity against infection with homologous and heterologous H5N1 influenza virus in mice. METHODS: In the present study, we evaluated a recombinant MVA vector expressing the HA gene of H5N1 influenza virus A/Vietnam/1194/04 (MVA-HA-VN/04) in nonhuman primates. Cynomolgus macaques were immunized twice and then were challenged with influenza virus A/Vietnam/1194/04 (clade 1) or A/Indonesia/5/05 (clade 2.1) to assess the level of protective immunity. RESULTS: Immunization with MVA-HA-VN/04 induced (cross-reactive) antibodies and prevented virus replication in the upper and lower respiratory tract and the development of severe necrotizing bronchointerstitial pneumonia. CONCLUSION: Therefore, MVA-HA-VN/04 is a promising vaccine candidate for the induction of protective immunity against highly pathogenic H5N1 avian influenza viruses in humans. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/19061423/Recombinant_modified_vaccinia_virus_Ankara_expressing_the_hemagglutinin_gene_confers_protection_against_homologous_and_heterologous_H5N1_influenza_virus_infections_in_macaques_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1086/595984 DB - PRIME DP - Unbound Medicine ER -