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Heart-cutting two-dimensional (size exclusion x reversed phase) liquid chromatography-mass spectrometry analysis of flavonol glycosides from leaves of Maytenus ilicifolia.
J Chromatogr A. 2009 Jan 02; 1216(1):99-105.JC

Abstract

Two-dimensional liquid chromatography-electrospray ionization mass spectrometry was employed to analyze flavonol glycosides present in leaves of Maytenus ilicifolia, frequently used in traditional Brazilian medicine. Since they contain many flavonol glycosides, including isomers, one-dimensional liquid chromatography did not give complete separation and identification, yielding overlapping of compounds with different molecular weights. Thus, employing size exclusion chromatography in the first and reversed phase in the second dimension, a great number of flavonol glycosides could be identified and its relative abundances determined. The majority of glycosides contained kaempferol or quercetin as aglycones, and glycosides with previously unreported structures were also present and characterized.

Authors+Show Affiliations

Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-980, Curitiba-PR, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19062030

Citation

de Souza, Lauro M., et al. "Heart-cutting Two-dimensional (size Exclusion X Reversed Phase) Liquid Chromatography-mass Spectrometry Analysis of Flavonol Glycosides From Leaves of Maytenus Ilicifolia." Journal of Chromatography. A, vol. 1216, no. 1, 2009, pp. 99-105.
de Souza LM, Cipriani TR, Sant'ana CF, et al. Heart-cutting two-dimensional (size exclusion x reversed phase) liquid chromatography-mass spectrometry analysis of flavonol glycosides from leaves of Maytenus ilicifolia. J Chromatogr A. 2009;1216(1):99-105.
de Souza, L. M., Cipriani, T. R., Sant'ana, C. F., Iacomini, M., Gorin, P. A., & Sassaki, G. L. (2009). Heart-cutting two-dimensional (size exclusion x reversed phase) liquid chromatography-mass spectrometry analysis of flavonol glycosides from leaves of Maytenus ilicifolia. Journal of Chromatography. A, 1216(1), 99-105. https://doi.org/10.1016/j.chroma.2008.11.032
de Souza LM, et al. Heart-cutting Two-dimensional (size Exclusion X Reversed Phase) Liquid Chromatography-mass Spectrometry Analysis of Flavonol Glycosides From Leaves of Maytenus Ilicifolia. J Chromatogr A. 2009 Jan 2;1216(1):99-105. PubMed PMID: 19062030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heart-cutting two-dimensional (size exclusion x reversed phase) liquid chromatography-mass spectrometry analysis of flavonol glycosides from leaves of Maytenus ilicifolia. AU - de Souza,Lauro M, AU - Cipriani,Thales R, AU - Sant'ana,Carolina F, AU - Iacomini,Marcello, AU - Gorin,Philip A J, AU - Sassaki,Guilherme L, Y1 - 2008/11/19/ PY - 2008/09/04/received PY - 2008/11/07/revised PY - 2008/11/13/accepted PY - 2008/12/9/pubmed PY - 2009/3/21/medline PY - 2008/12/9/entrez SP - 99 EP - 105 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 1 N2 - Two-dimensional liquid chromatography-electrospray ionization mass spectrometry was employed to analyze flavonol glycosides present in leaves of Maytenus ilicifolia, frequently used in traditional Brazilian medicine. Since they contain many flavonol glycosides, including isomers, one-dimensional liquid chromatography did not give complete separation and identification, yielding overlapping of compounds with different molecular weights. Thus, employing size exclusion chromatography in the first and reversed phase in the second dimension, a great number of flavonol glycosides could be identified and its relative abundances determined. The majority of glycosides contained kaempferol or quercetin as aglycones, and glycosides with previously unreported structures were also present and characterized. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/19062030/Heart_cutting_two_dimensional__size_exclusion_x_reversed_phase__liquid_chromatography_mass_spectrometry_analysis_of_flavonol_glycosides_from_leaves_of_Maytenus_ilicifolia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)02015-3 DB - PRIME DP - Unbound Medicine ER -