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Application of real-time RT-PCR to study gene expression in active dry yeast (ADY) during the rehydration phase.
Int J Food Microbiol. 2009 Jan 31; 129(1):30-6.IJ

Abstract

During the industrial production of active dry yeast (ADY) and its subsequent use in winemaking, the yeast cell is subjected to drastic environmental changes that force it to undergo extensive metabolic modifications and changes in gene expression. In this study, we describe the use of real-time reverse transcription-polymerase chain reaction (RT-PCR) to monitor gene expression in ADY Saccharomyces cerevisiae during rehydration in different media. We used three statistical approaches to investigate the expression stability of eight potential reference genes during the rehydration process. The reference system thus obtained was used to normalize the expression values of three genes codifying for the ammonium transporters -- MEP1, MEP2, and MEP3 -- and two genes involved in the osmotic response-SIP18 and GPD1. The results suggested that for the target genes tested, the yeast reacted immediately to rehydration only when a fermentable carbon source was present in the medium. Furthermore, MEP2 expression was modulated by the ammonium concentration, indicating that nitrogen catabolite repression (NCR) is active during the rehydration phase.

Authors+Show Affiliations

CRA-Centro di Ricerca per l'Enologia, Asti, Italy. biologia.molecolare@isenologia.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19062120

Citation

Vaudano, Enrico, et al. "Application of Real-time RT-PCR to Study Gene Expression in Active Dry Yeast (ADY) During the Rehydration Phase." International Journal of Food Microbiology, vol. 129, no. 1, 2009, pp. 30-6.
Vaudano E, Costantini A, Cersosimo M, et al. Application of real-time RT-PCR to study gene expression in active dry yeast (ADY) during the rehydration phase. Int J Food Microbiol. 2009;129(1):30-6.
Vaudano, E., Costantini, A., Cersosimo, M., Del Prete, V., & Garcia-Moruno, E. (2009). Application of real-time RT-PCR to study gene expression in active dry yeast (ADY) during the rehydration phase. International Journal of Food Microbiology, 129(1), 30-6. https://doi.org/10.1016/j.ijfoodmicro.2008.10.027
Vaudano E, et al. Application of Real-time RT-PCR to Study Gene Expression in Active Dry Yeast (ADY) During the Rehydration Phase. Int J Food Microbiol. 2009 Jan 31;129(1):30-6. PubMed PMID: 19062120.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of real-time RT-PCR to study gene expression in active dry yeast (ADY) during the rehydration phase. AU - Vaudano,Enrico, AU - Costantini,Antonella, AU - Cersosimo,Manuela, AU - Del Prete,Vincenzo, AU - Garcia-Moruno,Emilia, Y1 - 2008/11/05/ PY - 2008/07/02/received PY - 2008/10/21/revised PY - 2008/10/28/accepted PY - 2008/12/9/pubmed PY - 2009/4/15/medline PY - 2008/12/9/entrez SP - 30 EP - 6 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 129 IS - 1 N2 - During the industrial production of active dry yeast (ADY) and its subsequent use in winemaking, the yeast cell is subjected to drastic environmental changes that force it to undergo extensive metabolic modifications and changes in gene expression. In this study, we describe the use of real-time reverse transcription-polymerase chain reaction (RT-PCR) to monitor gene expression in ADY Saccharomyces cerevisiae during rehydration in different media. We used three statistical approaches to investigate the expression stability of eight potential reference genes during the rehydration process. The reference system thus obtained was used to normalize the expression values of three genes codifying for the ammonium transporters -- MEP1, MEP2, and MEP3 -- and two genes involved in the osmotic response-SIP18 and GPD1. The results suggested that for the target genes tested, the yeast reacted immediately to rehydration only when a fermentable carbon source was present in the medium. Furthermore, MEP2 expression was modulated by the ammonium concentration, indicating that nitrogen catabolite repression (NCR) is active during the rehydration phase. SN - 0168-1605 UR - https://www.unboundmedicine.com/medline/citation/19062120/Application_of_real_time_RT_PCR_to_study_gene_expression_in_active_dry_yeast__ADY__during_the_rehydration_phase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1605(08)00569-2 DB - PRIME DP - Unbound Medicine ER -