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High-speed separation and characterization of major constituents in Radix Paeoniae Rubra by fast high-performance liquid chromatography coupled with diode-array detection and time-of-flight mass spectrometry.
Rapid Commun Mass Spectrom. 2009 Jan; 23(1):119-30.RC

Abstract

A fast high-performance liquid chromatography (HPLC) method coupled with diode-array detection (DAD) and electrospray ionization time-of-flight mass spectrometry (ESI-TOFMS) has been developed for rapid separation and sensitive identification of major constituents in Radix Paeoniae Rubra (RPR). The total analysis time on a short column packed with 1.8-microm porous particles was about 20 min without a loss in resolution, six times faster than the performance of a conventional column analysis (115 min). The MS fragmentation behavior and structural characterization of major compounds in RPR were investigated here for the first time. The targets were rapidly screened from RPR matrix using a narrow mass window of 0.01 Da to restructure extracted ion chromatograms. Accurate mass measurements (less than 5 ppm error) for both the deprotonated molecule and characteristic fragment ions represent reliable identification criteria for these compounds in complex matrices with similar if not even better performance compared with tandem mass spectrometry. A total of 26 components were screened and identified in RPR including 11 monoterpene glycosides, 11 galloyl glucoses and 4 other phenolic compounds. From the point of time savings, resolving power, accurate mass measurement capability and full spectral sensitivity, the established fast HPLC/DAD/TOFMS method turns out to be a highly useful technique to identify constituents in complex herbal medicines.

Authors+Show Affiliations

Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), Ministry of Education, Nanjing 210009, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19065578

Citation

Liu, E-Hu, et al. "High-speed Separation and Characterization of Major Constituents in Radix Paeoniae Rubra By Fast High-performance Liquid Chromatography Coupled With Diode-array Detection and Time-of-flight Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 23, no. 1, 2009, pp. 119-30.
Liu EH, Qi LW, Li B, et al. High-speed separation and characterization of major constituents in Radix Paeoniae Rubra by fast high-performance liquid chromatography coupled with diode-array detection and time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2009;23(1):119-30.
Liu, E. H., Qi, L. W., Li, B., Peng, Y. B., Li, P., Li, C. Y., & Cao, J. (2009). High-speed separation and characterization of major constituents in Radix Paeoniae Rubra by fast high-performance liquid chromatography coupled with diode-array detection and time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 23(1), 119-30. https://doi.org/10.1002/rcm.3848
Liu EH, et al. High-speed Separation and Characterization of Major Constituents in Radix Paeoniae Rubra By Fast High-performance Liquid Chromatography Coupled With Diode-array Detection and Time-of-flight Mass Spectrometry. Rapid Commun Mass Spectrom. 2009;23(1):119-30. PubMed PMID: 19065578.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-speed separation and characterization of major constituents in Radix Paeoniae Rubra by fast high-performance liquid chromatography coupled with diode-array detection and time-of-flight mass spectrometry. AU - Liu,E-Hu, AU - Qi,Lian-Wen, AU - Li,Bin, AU - Peng,Yong-Bo, AU - Li,Ping, AU - Li,Chang-Yin, AU - Cao,Jun, PY - 2008/12/10/pubmed PY - 2009/3/13/medline PY - 2008/12/10/entrez SP - 119 EP - 30 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 23 IS - 1 N2 - A fast high-performance liquid chromatography (HPLC) method coupled with diode-array detection (DAD) and electrospray ionization time-of-flight mass spectrometry (ESI-TOFMS) has been developed for rapid separation and sensitive identification of major constituents in Radix Paeoniae Rubra (RPR). The total analysis time on a short column packed with 1.8-microm porous particles was about 20 min without a loss in resolution, six times faster than the performance of a conventional column analysis (115 min). The MS fragmentation behavior and structural characterization of major compounds in RPR were investigated here for the first time. The targets were rapidly screened from RPR matrix using a narrow mass window of 0.01 Da to restructure extracted ion chromatograms. Accurate mass measurements (less than 5 ppm error) for both the deprotonated molecule and characteristic fragment ions represent reliable identification criteria for these compounds in complex matrices with similar if not even better performance compared with tandem mass spectrometry. A total of 26 components were screened and identified in RPR including 11 monoterpene glycosides, 11 galloyl glucoses and 4 other phenolic compounds. From the point of time savings, resolving power, accurate mass measurement capability and full spectral sensitivity, the established fast HPLC/DAD/TOFMS method turns out to be a highly useful technique to identify constituents in complex herbal medicines. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/19065578/High_speed_separation_and_characterization_of_major_constituents_in_Radix_Paeoniae_Rubra_by_fast_high_performance_liquid_chromatography_coupled_with_diode_array_detection_and_time_of_flight_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.3848 DB - PRIME DP - Unbound Medicine ER -