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Genetic individualization of Cannabis sativa by a short tandem repeat multiplex system.
Anal Bioanal Chem 2009; 393(2):719-26AB

Abstract

Cannabis sativa is the most frequently used of all illicit drugs in the USA. Cannabis has been used throughout history for its stems in the production of hemp fiber, seed for oil and food, and buds and leaves as a psychoactive drug. Short tandem repeats (STRs) were chosen as molecular markers owing to their distinct advantages over other genetic methods. STRs are codominant, can be standardized such that reproducibility between laboratories can be easily achieved, have a high discrimination power, and can be multiplexed. In this study, six STR markers previously described for C. sativa were multiplexed into one reaction. The multiplex reaction was able to individualize 98 cannabis samples (14 hemp and 84 marijuana, authenticated as originating from 33 of the 50 states of the USA) and detect 29 alleles averaging 4.8 alleles per loci. The data did not relate the samples from the same state to each other. This is the first study to report a single-reaction sixplex and apply it to the analysis of almost 100 cannabis samples of known geographic origin.

Authors+Show Affiliations

Department of Chemistry and Biochemistry and International Forensic Research Institute, Florida International University, University Park, Miami, FL 33199, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19066867

Citation

Mendoza, Maria A., et al. "Genetic Individualization of Cannabis Sativa By a Short Tandem Repeat Multiplex System." Analytical and Bioanalytical Chemistry, vol. 393, no. 2, 2009, pp. 719-26.
Mendoza MA, Mills DK, Lata H, et al. Genetic individualization of Cannabis sativa by a short tandem repeat multiplex system. Anal Bioanal Chem. 2009;393(2):719-26.
Mendoza, M. A., Mills, D. K., Lata, H., Chandra, S., ElSohly, M. A., & Almirall, J. R. (2009). Genetic individualization of Cannabis sativa by a short tandem repeat multiplex system. Analytical and Bioanalytical Chemistry, 393(2), pp. 719-26. doi:10.1007/s00216-008-2500-3.
Mendoza MA, et al. Genetic Individualization of Cannabis Sativa By a Short Tandem Repeat Multiplex System. Anal Bioanal Chem. 2009;393(2):719-26. PubMed PMID: 19066867.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic individualization of Cannabis sativa by a short tandem repeat multiplex system. AU - Mendoza,Maria A, AU - Mills,DeEtta K, AU - Lata,Hemant, AU - Chandra,Suman, AU - ElSohly,Mahmoud A, AU - Almirall,Jose R, Y1 - 2008/12/06/ PY - 2008/09/09/received PY - 2008/10/27/accepted PY - 2008/10/22/revised PY - 2008/12/11/pubmed PY - 2009/2/12/medline PY - 2008/12/11/entrez SP - 719 EP - 26 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 393 IS - 2 N2 - Cannabis sativa is the most frequently used of all illicit drugs in the USA. Cannabis has been used throughout history for its stems in the production of hemp fiber, seed for oil and food, and buds and leaves as a psychoactive drug. Short tandem repeats (STRs) were chosen as molecular markers owing to their distinct advantages over other genetic methods. STRs are codominant, can be standardized such that reproducibility between laboratories can be easily achieved, have a high discrimination power, and can be multiplexed. In this study, six STR markers previously described for C. sativa were multiplexed into one reaction. The multiplex reaction was able to individualize 98 cannabis samples (14 hemp and 84 marijuana, authenticated as originating from 33 of the 50 states of the USA) and detect 29 alleles averaging 4.8 alleles per loci. The data did not relate the samples from the same state to each other. This is the first study to report a single-reaction sixplex and apply it to the analysis of almost 100 cannabis samples of known geographic origin. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/19066867/abstract/Genetic_individualization_of_Cannabis_sativa_by_a_short_tandem_repeat_multiplex_system_ L2 - https://dx.doi.org/10.1007/s00216-008-2500-3 DB - PRIME DP - Unbound Medicine ER -