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Simultaneous total antioxidant capacity assay of lipophilic and hydrophilic antioxidants in the same acetone-water solution containing 2% methyl-beta-cyclodextrin using the cupric reducing antioxidant capacity (CUPRAC) method.
Anal Chim Acta. 2008 Dec 07; 630(1):28-39.AC

Abstract

Antioxidants are health beneficial compounds that can protect cells from the damage caused by unstable molecules known as reactive oxygen species (ROS). This work reports the capacity assay of both lipophilic and hydrophilic antioxidants simultaneously, by making use of their 'host-guest' complexes with methyl-beta-cyclodextrin (M-beta-CD), a cyclic oligosaccharide, in acetonated aqueous medium using the cupric reducing antioxidant capacity (CUPRAC) method. Thus the order of antioxidant potency of various compounds irrespective of their lipophilicity could be established in the same solvent medium. M-beta-CD was introduced as the water solubility enhancer for lipophilic antioxidants. Two percent M-beta-CD (w/v) in an acetone-H(2)O (9:1, v/v) mixture was found to sufficiently solubilize beta-carotene, lycopene, vitamin E, vitamin C, synthetic antioxidants and other phenolic antioxidants. This assay was validated through linearity, additivity, precision, and recovery. The validation results demonstrate that the CUPRAC assay is reliable and robust. In acetonated aqueous solution of M-beta-CD, only CUPRAC and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays were capable of measuring carotenoids together with hydrophilic antioxidants. The CUPRAC antioxidant capacities of a wide range of polyphenolics and flavonoids were experimentally reported in this work as trolox equivalent antioxidant capacity (TEAC) in the CUPRAC assay, and compared to those found by reference methods, ABTS/horseradish peroxidase (HRP)-H(2)O(2) and ferric reducing antioxidant power (FRAP) assays.

Authors+Show Affiliations

Department of Chemistry, Faculty of Engineering, Istanbul University, Avcilar 34320, Istanbul, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19068323

Citation

Ozyürek, Mustafa, et al. "Simultaneous Total Antioxidant Capacity Assay of Lipophilic and Hydrophilic Antioxidants in the Same Acetone-water Solution Containing 2% Methyl-beta-cyclodextrin Using the Cupric Reducing Antioxidant Capacity (CUPRAC) Method." Analytica Chimica Acta, vol. 630, no. 1, 2008, pp. 28-39.
Ozyürek M, Bektaşoğlu B, Güçlü K, et al. Simultaneous total antioxidant capacity assay of lipophilic and hydrophilic antioxidants in the same acetone-water solution containing 2% methyl-beta-cyclodextrin using the cupric reducing antioxidant capacity (CUPRAC) method. Anal Chim Acta. 2008;630(1):28-39.
Ozyürek, M., Bektaşoğlu, B., Güçlü, K., Güngör, N., & Apak, R. (2008). Simultaneous total antioxidant capacity assay of lipophilic and hydrophilic antioxidants in the same acetone-water solution containing 2% methyl-beta-cyclodextrin using the cupric reducing antioxidant capacity (CUPRAC) method. Analytica Chimica Acta, 630(1), 28-39. https://doi.org/10.1016/j.aca.2008.09.057
Ozyürek M, et al. Simultaneous Total Antioxidant Capacity Assay of Lipophilic and Hydrophilic Antioxidants in the Same Acetone-water Solution Containing 2% Methyl-beta-cyclodextrin Using the Cupric Reducing Antioxidant Capacity (CUPRAC) Method. Anal Chim Acta. 2008 Dec 7;630(1):28-39. PubMed PMID: 19068323.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous total antioxidant capacity assay of lipophilic and hydrophilic antioxidants in the same acetone-water solution containing 2% methyl-beta-cyclodextrin using the cupric reducing antioxidant capacity (CUPRAC) method. AU - Ozyürek,Mustafa, AU - Bektaşoğlu,Burcu, AU - Güçlü,Kubilay, AU - Güngör,Nilay, AU - Apak,Reşat, Y1 - 2008/10/08/ PY - 2008/07/26/received PY - 2008/09/19/revised PY - 2008/09/23/accepted PY - 2008/12/11/pubmed PY - 2009/2/14/medline PY - 2008/12/11/entrez SP - 28 EP - 39 JF - Analytica chimica acta JO - Anal. Chim. Acta VL - 630 IS - 1 N2 - Antioxidants are health beneficial compounds that can protect cells from the damage caused by unstable molecules known as reactive oxygen species (ROS). This work reports the capacity assay of both lipophilic and hydrophilic antioxidants simultaneously, by making use of their 'host-guest' complexes with methyl-beta-cyclodextrin (M-beta-CD), a cyclic oligosaccharide, in acetonated aqueous medium using the cupric reducing antioxidant capacity (CUPRAC) method. Thus the order of antioxidant potency of various compounds irrespective of their lipophilicity could be established in the same solvent medium. M-beta-CD was introduced as the water solubility enhancer for lipophilic antioxidants. Two percent M-beta-CD (w/v) in an acetone-H(2)O (9:1, v/v) mixture was found to sufficiently solubilize beta-carotene, lycopene, vitamin E, vitamin C, synthetic antioxidants and other phenolic antioxidants. This assay was validated through linearity, additivity, precision, and recovery. The validation results demonstrate that the CUPRAC assay is reliable and robust. In acetonated aqueous solution of M-beta-CD, only CUPRAC and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays were capable of measuring carotenoids together with hydrophilic antioxidants. The CUPRAC antioxidant capacities of a wide range of polyphenolics and flavonoids were experimentally reported in this work as trolox equivalent antioxidant capacity (TEAC) in the CUPRAC assay, and compared to those found by reference methods, ABTS/horseradish peroxidase (HRP)-H(2)O(2) and ferric reducing antioxidant power (FRAP) assays. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/19068323/Simultaneous_total_antioxidant_capacity_assay_of_lipophilic_and_hydrophilic_antioxidants_in_the_same_acetone_water_solution_containing_2_methyl_beta_cyclodextrin_using_the_cupric_reducing_antioxidant_capacity__CUPRAC__method_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(08)01703-0 DB - PRIME DP - Unbound Medicine ER -