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[Identification of periodontopathogen microorganisms by PCR technique].
Srp Arh Celok Lek. 2008 Sep-Oct; 136(9-10):476-80.SA

Abstract

INTRODUCTION

Periodontitis is an inflammatory disease of the supporting tissues of teeth and is a major cause of tooth loss in adults. The onset and progression of periodontal disease is attributed to the presence of elevated levels of a consortium of pathogenic bacteria. Gram negative bacteria, mainly strict anaerobes, play the major role.

OBJECTIVE

The present study aimed to assess the presence of the main types of microorganisms involved in the aetiopathogenesis of periodontal disease: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia and Prevotella intermedia in different samples collected from the oral cavity of 90 patients diagnosed with periodontitis.

METHOD

Bacterial DNA detection was performed in diverse biological materials, namely in dental plaque, gingival tissue and saliva, by means of multiplex PCR, a technique that allows simultaneous identification of two different bacterial genomes.

RESULTS

In the dental plaque of the periodontitis patients, Treponema denticola dominated. In the gingival tissue, Tannerella forsythia and Treponema denticola were the microbiota most frequently detected, whilst in saliva Treponema denticola and Eikenella corrodens were found with the highest percentage.

CONCLUSION

The identification of microorganisms by multiplex PCR is specific and sensitive. Rapid and precise assessment of different types of periodontopathogens is extremely important for early detection of the infection and consequently for the prevention and treatment of periodontal disease. In everyday clinical practice, for routine bacterial evaluation in patients with periodontal disease, the dental plaque is the most suitable biological material, because it is the richest in periodontal bacteria.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

srp

PubMed ID

19069337

Citation

Milićević, Radovan, et al. "[Identification of Periodontopathogen Microorganisms By PCR Technique]." Srpski Arhiv Za Celokupno Lekarstvo, vol. 136, no. 9-10, 2008, pp. 476-80.
Milićević R, Brajović G, Nikolić-Jakoba N, et al. [Identification of periodontopathogen microorganisms by PCR technique]. Srp Arh Celok Lek. 2008;136(9-10):476-80.
Milićević, R., Brajović, G., Nikolić-Jakoba, N., Popović, B., Pavlica, D., Leković, V., & Milasin, J. (2008). [Identification of periodontopathogen microorganisms by PCR technique]. Srpski Arhiv Za Celokupno Lekarstvo, 136(9-10), 476-80.
Milićević R, et al. [Identification of Periodontopathogen Microorganisms By PCR Technique]. Srp Arh Celok Lek. 2008 Sep-Oct;136(9-10):476-80. PubMed PMID: 19069337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Identification of periodontopathogen microorganisms by PCR technique]. AU - Milićević,Radovan, AU - Brajović,Gavrilo, AU - Nikolić-Jakoba,Natasa, AU - Popović,Branka, AU - Pavlica,Dusan, AU - Leković,Vojislav, AU - Milasin,Jelena, PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/1/28/medline SP - 476 EP - 80 JF - Srpski arhiv za celokupno lekarstvo JO - Srp Arh Celok Lek VL - 136 IS - 9-10 N2 - INTRODUCTION: Periodontitis is an inflammatory disease of the supporting tissues of teeth and is a major cause of tooth loss in adults. The onset and progression of periodontal disease is attributed to the presence of elevated levels of a consortium of pathogenic bacteria. Gram negative bacteria, mainly strict anaerobes, play the major role. OBJECTIVE: The present study aimed to assess the presence of the main types of microorganisms involved in the aetiopathogenesis of periodontal disease: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia and Prevotella intermedia in different samples collected from the oral cavity of 90 patients diagnosed with periodontitis. METHOD: Bacterial DNA detection was performed in diverse biological materials, namely in dental plaque, gingival tissue and saliva, by means of multiplex PCR, a technique that allows simultaneous identification of two different bacterial genomes. RESULTS: In the dental plaque of the periodontitis patients, Treponema denticola dominated. In the gingival tissue, Tannerella forsythia and Treponema denticola were the microbiota most frequently detected, whilst in saliva Treponema denticola and Eikenella corrodens were found with the highest percentage. CONCLUSION: The identification of microorganisms by multiplex PCR is specific and sensitive. Rapid and precise assessment of different types of periodontopathogens is extremely important for early detection of the infection and consequently for the prevention and treatment of periodontal disease. In everyday clinical practice, for routine bacterial evaluation in patients with periodontal disease, the dental plaque is the most suitable biological material, because it is the richest in periodontal bacteria. SN - 0370-8179 UR - https://www.unboundmedicine.com/medline/citation/19069337/[Identification_of_periodontopathogen_microorganisms_by_PCR_technique]_ L2 - http://srpskiarhiv.rs/global/pdf/articles-2008/septembar-oktobar/IdentificationofperiodontopathogenmicroorganismsbyPCRtechnique.pdf DB - PRIME DP - Unbound Medicine ER -