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Cannabidiol decreases bone resorption by inhibiting RANK/RANKL expression and pro-inflammatory cytokines during experimental periodontitis in rats.

Abstract

Cannabidiol (CBD) is a cannabinoid component from Cannabis sativa that does not induce psychotomimetic effects and possess anti-inflammatory properties. In the present study we tested the effects of CBD in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying these effects. Periodontal disease was induced by a ligature placed around the mandible first molars of each animal. Male Wistar rats were divided into 3 groups: control animals; ligature-induced animals treated with vehicle and ligature-induced animals treated with CBD (5 mg/kg, daily). Thirty days after the induction of periodontal disease the animals were sacrificed and mandibles and gingival tissues removed for further analysis. Morphometrical analysis of alveolar bone loss demonstrated that CBD-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-kappaB ligand RANKL/RANK. Moreover, gingival tissues from the CBD-treated group showed decreased neutrophil migration (MPO assay) associated with lower interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha production. These results indicate that CBD may be useful to control bone resorption during progression of experimental periodontitis in rats.

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  • Authors+Show Affiliations

    ,

    Laboratory of Molecular Biology, University of Uberaba, Brazil. marcelo.napimoga@uniube.br

    , , , , , , ,

    Source

    International immunopharmacology 9:2 2009 Feb pg 216-22

    MeSH

    Animals
    Anti-Inflammatory Agents, Non-Steroidal
    Bone Resorption
    Cannabidiol
    Cell Movement
    Cytokines
    Disease Models, Animal
    Male
    Neutrophils
    Periodontitis
    Peroxidase
    RANK Ligand
    Rats
    Rats, Wistar
    Receptor Activator of Nuclear Factor-kappa B

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    19070683

    Citation

    Napimoga, Marcelo H., et al. "Cannabidiol Decreases Bone Resorption By Inhibiting RANK/RANKL Expression and Pro-inflammatory Cytokines During Experimental Periodontitis in Rats." International Immunopharmacology, vol. 9, no. 2, 2009, pp. 216-22.
    Napimoga MH, Benatti BB, Lima FO, et al. Cannabidiol decreases bone resorption by inhibiting RANK/RANKL expression and pro-inflammatory cytokines during experimental periodontitis in rats. Int Immunopharmacol. 2009;9(2):216-22.
    Napimoga, M. H., Benatti, B. B., Lima, F. O., Alves, P. M., Campos, A. C., Pena-Dos-Santos, D. R., ... Guimarães, F. S. (2009). Cannabidiol decreases bone resorption by inhibiting RANK/RANKL expression and pro-inflammatory cytokines during experimental periodontitis in rats. International Immunopharmacology, 9(2), pp. 216-22. doi:10.1016/j.intimp.2008.11.010.
    Napimoga MH, et al. Cannabidiol Decreases Bone Resorption By Inhibiting RANK/RANKL Expression and Pro-inflammatory Cytokines During Experimental Periodontitis in Rats. Int Immunopharmacol. 2009;9(2):216-22. PubMed PMID: 19070683.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Cannabidiol decreases bone resorption by inhibiting RANK/RANKL expression and pro-inflammatory cytokines during experimental periodontitis in rats. AU - Napimoga,Marcelo H, AU - Benatti,Bruno B, AU - Lima,Flavia O, AU - Alves,Polyanna M, AU - Campos,Alline C, AU - Pena-Dos-Santos,Diego R, AU - Severino,Fernando P, AU - Cunha,Fernando Q, AU - Guimarães,Francisco S, Y1 - 2008/12/12/ PY - 2008/08/25/received PY - 2008/11/12/revised PY - 2008/11/13/accepted PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/5/1/medline SP - 216 EP - 22 JF - International immunopharmacology JO - Int. Immunopharmacol. VL - 9 IS - 2 N2 - Cannabidiol (CBD) is a cannabinoid component from Cannabis sativa that does not induce psychotomimetic effects and possess anti-inflammatory properties. In the present study we tested the effects of CBD in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying these effects. Periodontal disease was induced by a ligature placed around the mandible first molars of each animal. Male Wistar rats were divided into 3 groups: control animals; ligature-induced animals treated with vehicle and ligature-induced animals treated with CBD (5 mg/kg, daily). Thirty days after the induction of periodontal disease the animals were sacrificed and mandibles and gingival tissues removed for further analysis. Morphometrical analysis of alveolar bone loss demonstrated that CBD-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-kappaB ligand RANKL/RANK. Moreover, gingival tissues from the CBD-treated group showed decreased neutrophil migration (MPO assay) associated with lower interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha production. These results indicate that CBD may be useful to control bone resorption during progression of experimental periodontitis in rats. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/19070683/abstract/Cannabidiol_decreases_bone_resorption_by_inhibiting_RANK/RANKL_expression_and_pro_inflammatory_cytokines_during_experimental_periodontitis_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(08)00346-9 DB - PRIME DP - Unbound Medicine ER -