Tags

Type your tag names separated by a space and hit enter

Ultrasensitive electrogenerated chemiluminescence detection of DNA hybridization using carbon-nanotubes loaded with tris(2,2'-bipyridyl) ruthenium derivative tags.
Talanta. 2007 Jul 31; 72(5):1704-9.T

Abstract

An ultrasensitive electrogenerated chemiluminescence (ECL) detection method of DNA hybridization based on single-walled carbon-nanotubes (SWNT) carrying a large number of ruthenium complex tags was developed. The probe single strand DNA (ss-DNA) and ruthenium complex were loaded at SWNT, which was taken as an ECL probe. When the capture ss-DNA with a thiol group was self-assembled onto the surface of gold electrode, and then hybridized with target ss-DNA and further hybridized with the ECL probe to form DNA sandwich conjugate, a strong ECL response was electrochemically generated. The ECL intensity was linearly related to the concentration of perfect-matched target ss-DNA in the range from 2.4x10(-14) to 1.7x10(-12)M with a detection limit of 9.0x10(-15)M. The ECL signal difference permitted to discriminate the perfect-matched target ss-DNA and two-base-mismatched ss-DNA. This work demonstrates that SWNT can provide an amplification platform for carrying a large number of ECL probe and thus resulting in an ultrasensitive ECL detection of DNA hybridization.

Authors+Show Affiliations

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, PR China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19071820

Citation

Li, Yan, et al. "Ultrasensitive Electrogenerated Chemiluminescence Detection of DNA Hybridization Using Carbon-nanotubes Loaded With Tris(2,2'-bipyridyl) Ruthenium Derivative Tags." Talanta, vol. 72, no. 5, 2007, pp. 1704-9.
Li Y, Qi H, Fang F, et al. Ultrasensitive electrogenerated chemiluminescence detection of DNA hybridization using carbon-nanotubes loaded with tris(2,2'-bipyridyl) ruthenium derivative tags. Talanta. 2007;72(5):1704-9.
Li, Y., Qi, H., Fang, F., & Zhang, C. (2007). Ultrasensitive electrogenerated chemiluminescence detection of DNA hybridization using carbon-nanotubes loaded with tris(2,2'-bipyridyl) ruthenium derivative tags. Talanta, 72(5), 1704-9. https://doi.org/10.1016/j.talanta.2007.01.062
Li Y, et al. Ultrasensitive Electrogenerated Chemiluminescence Detection of DNA Hybridization Using Carbon-nanotubes Loaded With Tris(2,2'-bipyridyl) Ruthenium Derivative Tags. Talanta. 2007 Jul 31;72(5):1704-9. PubMed PMID: 19071820.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrasensitive electrogenerated chemiluminescence detection of DNA hybridization using carbon-nanotubes loaded with tris(2,2'-bipyridyl) ruthenium derivative tags. AU - Li,Yan, AU - Qi,Honglan, AU - Fang,Fang, AU - Zhang,Chengxiao, Y1 - 2007/02/09/ PY - 2006/09/02/received PY - 2007/01/21/revised PY - 2007/01/26/accepted PY - 2008/12/17/entrez PY - 2007/7/31/pubmed PY - 2007/7/31/medline SP - 1704 EP - 9 JF - Talanta JO - Talanta VL - 72 IS - 5 N2 - An ultrasensitive electrogenerated chemiluminescence (ECL) detection method of DNA hybridization based on single-walled carbon-nanotubes (SWNT) carrying a large number of ruthenium complex tags was developed. The probe single strand DNA (ss-DNA) and ruthenium complex were loaded at SWNT, which was taken as an ECL probe. When the capture ss-DNA with a thiol group was self-assembled onto the surface of gold electrode, and then hybridized with target ss-DNA and further hybridized with the ECL probe to form DNA sandwich conjugate, a strong ECL response was electrochemically generated. The ECL intensity was linearly related to the concentration of perfect-matched target ss-DNA in the range from 2.4x10(-14) to 1.7x10(-12)M with a detection limit of 9.0x10(-15)M. The ECL signal difference permitted to discriminate the perfect-matched target ss-DNA and two-base-mismatched ss-DNA. This work demonstrates that SWNT can provide an amplification platform for carrying a large number of ECL probe and thus resulting in an ultrasensitive ECL detection of DNA hybridization. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/19071820/Ultrasensitive_electrogenerated_chemiluminescence_detection_of_DNA_hybridization_using_carbon_nanotubes_loaded_with_tris_22'_bipyridyl__ruthenium_derivative_tags_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(07)00105-1 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.