TY - JOUR T1 - Ultrasensitive electrogenerated chemiluminescence detection of DNA hybridization using carbon-nanotubes loaded with tris(2,2'-bipyridyl) ruthenium derivative tags. AU - Li,Yan, AU - Qi,Honglan, AU - Fang,Fang, AU - Zhang,Chengxiao, Y1 - 2007/02/09/ PY - 2006/09/02/received PY - 2007/01/21/revised PY - 2007/01/26/accepted PY - 2008/12/17/entrez PY - 2007/7/31/pubmed PY - 2007/7/31/medline SP - 1704 EP - 9 JF - Talanta JO - Talanta VL - 72 IS - 5 N2 - An ultrasensitive electrogenerated chemiluminescence (ECL) detection method of DNA hybridization based on single-walled carbon-nanotubes (SWNT) carrying a large number of ruthenium complex tags was developed. The probe single strand DNA (ss-DNA) and ruthenium complex were loaded at SWNT, which was taken as an ECL probe. When the capture ss-DNA with a thiol group was self-assembled onto the surface of gold electrode, and then hybridized with target ss-DNA and further hybridized with the ECL probe to form DNA sandwich conjugate, a strong ECL response was electrochemically generated. The ECL intensity was linearly related to the concentration of perfect-matched target ss-DNA in the range from 2.4x10(-14) to 1.7x10(-12)M with a detection limit of 9.0x10(-15)M. The ECL signal difference permitted to discriminate the perfect-matched target ss-DNA and two-base-mismatched ss-DNA. This work demonstrates that SWNT can provide an amplification platform for carrying a large number of ECL probe and thus resulting in an ultrasensitive ECL detection of DNA hybridization. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/19071820/Ultrasensitive_electrogenerated_chemiluminescence_detection_of_DNA_hybridization_using_carbon_nanotubes_loaded_with_tris_22'_bipyridyl__ruthenium_derivative_tags_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(07)00105-1 DB - PRIME DP - Unbound Medicine ER -