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Aptamer-functionalized gold nanoparticles as probes in a dry-reagent strip biosensor for protein analysis.
Anal Chem. 2009 Jan 15; 81(2):669-75.AC

Abstract

The highly specific molecular recognition properties of aptamers are combined with the unique optical properties of gold nanoparticles for the development of a dry-reagent strip biosensor that enables qualitative (visual)/quantitative detection of protein within minutes. A model system comprising thrombin as an analyte and a pair of aptamer probes is used to demonstrate the proof-of-concept on the conventional lateral flow test strip. The assay avoids the multiple incubation and washing steps performed in most current aptamer-based protein analyses. Although qualitative tests are realized by observing the color change of the test zone, quantitative data are obtained by recording the optical responses of the test zone with a portable "strip reader". The response of the biosensor is linear over the range of 5-100 nM of thrombin with a detection limit of 2.5 nM (S/N = 3). By comparing the analytical performances of the aptamer-based strip biosensor with the antibody-based strip sensor, we can demonstrate that aptamers are equivalent or superior to antibodies in terms of specificity and sensitivity, respectively. The sensor was used successfully for detection of thrombin in human plasma samples. It shows great promise for use of aptamer-functionalized gold nanoparticle probes in dry-reagent strip biosensors for point-of-care or in-field detection of proteins.

Authors+Show Affiliations

College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, P. R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19072289

Citation

Xu, Hui, et al. "Aptamer-functionalized Gold Nanoparticles as Probes in a Dry-reagent Strip Biosensor for Protein Analysis." Analytical Chemistry, vol. 81, no. 2, 2009, pp. 669-75.
Xu H, Mao X, Zeng Q, et al. Aptamer-functionalized gold nanoparticles as probes in a dry-reagent strip biosensor for protein analysis. Anal Chem. 2009;81(2):669-75.
Xu, H., Mao, X., Zeng, Q., Wang, S., Kawde, A. N., & Liu, G. (2009). Aptamer-functionalized gold nanoparticles as probes in a dry-reagent strip biosensor for protein analysis. Analytical Chemistry, 81(2), 669-75. https://doi.org/10.1021/ac8020592
Xu H, et al. Aptamer-functionalized Gold Nanoparticles as Probes in a Dry-reagent Strip Biosensor for Protein Analysis. Anal Chem. 2009 Jan 15;81(2):669-75. PubMed PMID: 19072289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aptamer-functionalized gold nanoparticles as probes in a dry-reagent strip biosensor for protein analysis. AU - Xu,Hui, AU - Mao,Xun, AU - Zeng,Qingxiang, AU - Wang,Shengfu, AU - Kawde,Abdel-Nasser, AU - Liu,Guodong, PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/2/21/medline SP - 669 EP - 75 JF - Analytical chemistry JO - Anal Chem VL - 81 IS - 2 N2 - The highly specific molecular recognition properties of aptamers are combined with the unique optical properties of gold nanoparticles for the development of a dry-reagent strip biosensor that enables qualitative (visual)/quantitative detection of protein within minutes. A model system comprising thrombin as an analyte and a pair of aptamer probes is used to demonstrate the proof-of-concept on the conventional lateral flow test strip. The assay avoids the multiple incubation and washing steps performed in most current aptamer-based protein analyses. Although qualitative tests are realized by observing the color change of the test zone, quantitative data are obtained by recording the optical responses of the test zone with a portable "strip reader". The response of the biosensor is linear over the range of 5-100 nM of thrombin with a detection limit of 2.5 nM (S/N = 3). By comparing the analytical performances of the aptamer-based strip biosensor with the antibody-based strip sensor, we can demonstrate that aptamers are equivalent or superior to antibodies in terms of specificity and sensitivity, respectively. The sensor was used successfully for detection of thrombin in human plasma samples. It shows great promise for use of aptamer-functionalized gold nanoparticle probes in dry-reagent strip biosensors for point-of-care or in-field detection of proteins. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/19072289/Aptamer_functionalized_gold_nanoparticles_as_probes_in_a_dry_reagent_strip_biosensor_for_protein_analysis_ L2 - https://doi.org/10.1021/ac8020592 DB - PRIME DP - Unbound Medicine ER -