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Characterization of two isoforms of Japanese spiny lobster Panulirus japonicus defensin cDNA.
Dev Comp Immunol 2009; 33(4):434-8DC

Abstract

Antimicrobial peptides (AMPs) are components of the innate immune responses that form the first line of host defense against pathogens. In this study, cDNAs of two new isoforms of defensin (designated PJD1 and PJD2) from a Japanese spiny lobster Panulirus japonicus haemocytes cDNA library were cloned and sequenced. PJD1 and PJD2 consist of 656 and 673 nucleotides encoding putative proteins of 66 and 64 amino acids, respectively. The isoforms share 74.2% amino acid identity. In a phylogenetic analysis, the peptides clustered with vertebrate defensins and were closely mostly related to chicken beta-defensin. PJD1 and PJD2 were detected in all tissues examined including heart, nerves, intestine, haemocytes, gills and hepatopancreas.

Authors+Show Affiliations

Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19073210

Citation

Pisuttharachai, Duangjai, et al. "Characterization of Two Isoforms of Japanese Spiny Lobster Panulirus Japonicus Defensin CDNA." Developmental and Comparative Immunology, vol. 33, no. 4, 2009, pp. 434-8.
Pisuttharachai D, Yasuike M, Aono H, et al. Characterization of two isoforms of Japanese spiny lobster Panulirus japonicus defensin cDNA. Dev Comp Immunol. 2009;33(4):434-8.
Pisuttharachai, D., Yasuike, M., Aono, H., Yano, Y., Murakami, K., Kondo, H., ... Hirono, I. (2009). Characterization of two isoforms of Japanese spiny lobster Panulirus japonicus defensin cDNA. Developmental and Comparative Immunology, 33(4), pp. 434-8. doi:10.1016/j.dci.2008.11.007.
Pisuttharachai D, et al. Characterization of Two Isoforms of Japanese Spiny Lobster Panulirus Japonicus Defensin CDNA. Dev Comp Immunol. 2009;33(4):434-8. PubMed PMID: 19073210.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of two isoforms of Japanese spiny lobster Panulirus japonicus defensin cDNA. AU - Pisuttharachai,Duangjai, AU - Yasuike,Motoshige, AU - Aono,Hideaki, AU - Yano,Yutaka, AU - Murakami,Keisuke, AU - Kondo,Hidehiro, AU - Aoki,Takashi, AU - Hirono,Ikuo, Y1 - 2008/12/13/ PY - 2008/10/01/received PY - 2008/11/18/revised PY - 2008/11/19/accepted PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/3/13/medline SP - 434 EP - 8 JF - Developmental and comparative immunology JO - Dev. Comp. Immunol. VL - 33 IS - 4 N2 - Antimicrobial peptides (AMPs) are components of the innate immune responses that form the first line of host defense against pathogens. In this study, cDNAs of two new isoforms of defensin (designated PJD1 and PJD2) from a Japanese spiny lobster Panulirus japonicus haemocytes cDNA library were cloned and sequenced. PJD1 and PJD2 consist of 656 and 673 nucleotides encoding putative proteins of 66 and 64 amino acids, respectively. The isoforms share 74.2% amino acid identity. In a phylogenetic analysis, the peptides clustered with vertebrate defensins and were closely mostly related to chicken beta-defensin. PJD1 and PJD2 were detected in all tissues examined including heart, nerves, intestine, haemocytes, gills and hepatopancreas. SN - 1879-0089 UR - https://www.unboundmedicine.com/medline/citation/19073210/Characterization_of_two_isoforms_of_Japanese_spiny_lobster_Panulirus_japonicus_defensin_cDNA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0145-305X(08)00271-1 DB - PRIME DP - Unbound Medicine ER -