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RETRACTED ARTICLE

Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified alpha-linolenic acid in unanesthetized rats.
J Lipid Res. 2009 Apr; 50(4):749-58.JL

Abstract

Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), long-chain n-3 PUFAs important for brain and heart function, can be obtained from dietary fish products or by liver synthesis from alpha-linolenic acid (alpha-LNA). Their daily human dietary requirements are not clear, and their liver synthesis rates in humans and nonhumans are unknown. We estimated whole-body (presumably liver) synthesis rates in unanesthetized rats by infusing [U-(13)C]alpha-LNA intravenously for 2 h and measuring labeled and unlabeled n-3 PUFA in arterial plasma using negative chemical ionization GC-MS. Newly synthesized esterified [(13)C]DHA, [(13)C]EPA, and [(13)C]docosapentaenoic acid (DPA) appeared in arterial plasma after 60 min of infusion, then their concentrations rose in an S-shaped manner. Esterified concentration x plasma volume data were fit with a sigmoidal equation, whose peak first derivatives provided synthesis rates of unlabeled EPA, DPA, and DHA equal to 8.40, 6.27, and 9.84 mumol/day, respectively. The DHA synthesis rate exceeded the published daily rat brain DHA consumption rate by 30-fold, suggesting that liver synthesis from alpha-LNA could maintain brain DHA homeostasis were DHA absent from the diet. This stable isotope infusion method could be used to quantify whole-body DHA synthesis rates in human subjects.

Authors+Show Affiliations

Brain Physiology and Metabolism Section, National Institute on Aging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA. gaof@mail.nih.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Retracted Publication

Language

eng

PubMed ID

19074373

Citation

Gao, Fei, et al. "Whole-body Synthesis-secretion Rates of Long-chain N-3 PUFAs From Circulating Unesterified Alpha-linolenic Acid in Unanesthetized Rats." Journal of Lipid Research, vol. 50, no. 4, 2009, pp. 749-58.
Gao F, Kiesewetter D, Chang L, et al. Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified alpha-linolenic acid in unanesthetized rats. J Lipid Res. 2009;50(4):749-58.
Gao, F., Kiesewetter, D., Chang, L., Ma, K., Bell, J. M., Rapoport, S. I., & Igarashi, M. (2009). Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified alpha-linolenic acid in unanesthetized rats. Journal of Lipid Research, 50(4), 749-58. https://doi.org/10.1194/jlr.D800056-JLR200
Gao F, et al. Whole-body Synthesis-secretion Rates of Long-chain N-3 PUFAs From Circulating Unesterified Alpha-linolenic Acid in Unanesthetized Rats. J Lipid Res. 2009;50(4):749-58. PubMed PMID: 19074373.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified alpha-linolenic acid in unanesthetized rats. AU - Gao,Fei, AU - Kiesewetter,Dale, AU - Chang,Lisa, AU - Ma,Kaizong, AU - Bell,Jane M, AU - Rapoport,Stanley I, AU - Igarashi,Miki, Y1 - 2008/12/11/ PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/6/23/medline SP - 749 EP - 58 JF - Journal of lipid research JO - J Lipid Res VL - 50 IS - 4 N2 - Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), long-chain n-3 PUFAs important for brain and heart function, can be obtained from dietary fish products or by liver synthesis from alpha-linolenic acid (alpha-LNA). Their daily human dietary requirements are not clear, and their liver synthesis rates in humans and nonhumans are unknown. We estimated whole-body (presumably liver) synthesis rates in unanesthetized rats by infusing [U-(13)C]alpha-LNA intravenously for 2 h and measuring labeled and unlabeled n-3 PUFA in arterial plasma using negative chemical ionization GC-MS. Newly synthesized esterified [(13)C]DHA, [(13)C]EPA, and [(13)C]docosapentaenoic acid (DPA) appeared in arterial plasma after 60 min of infusion, then their concentrations rose in an S-shaped manner. Esterified concentration x plasma volume data were fit with a sigmoidal equation, whose peak first derivatives provided synthesis rates of unlabeled EPA, DPA, and DHA equal to 8.40, 6.27, and 9.84 mumol/day, respectively. The DHA synthesis rate exceeded the published daily rat brain DHA consumption rate by 30-fold, suggesting that liver synthesis from alpha-LNA could maintain brain DHA homeostasis were DHA absent from the diet. This stable isotope infusion method could be used to quantify whole-body DHA synthesis rates in human subjects. SN - 0022-2275 UR - https://www.unboundmedicine.com/medline/citation/19074373/Whole_body_synthesis_secretion_rates_of_long_chain_n_3_PUFAs_from_circulating_unesterified_alpha_linolenic_acid_in_unanesthetized_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2275(20)30873-7 DB - PRIME DP - Unbound Medicine ER -