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Menin promotes the Wnt signaling pathway in pancreatic endocrine cells.
Mol Cancer Res. 2008 Dec; 6(12):1894-907.MC

Abstract

Menin is a tumor suppressor protein mutated in patients with multiple endocrine neoplasia type 1. We show that menin is essential for canonical Wnt/beta-catenin signaling in cultured rodent islet tumor cells. In these cells, overexpression of menin significantly enhances TCF gene assay reporter activity in response to beta-catenin activation. Contrastingly, inhibition of menin expression with Men1 siRNA decreases TCF reporter gene activity. Likewise, multiple endocrine neoplasia type 1 disease associated missense mutations of menin abrogate the ability to increase TCF reporter gene activity. We show that menin physically interacts with proteins involved in the canonical Wnt signaling pathway, including beta-catenin, TCF3 (TCFL1), and weakly with TCF4 (TCFL2). Menin overexpression increases expression of the Wnt/beta-catenin downstream target gene Axin2, which is associated with increased H3K4 trimethylation of the Axin2 gene promoter. Moreover, inhibition of menin expression by siRNA abrogates H3K4 trimethylation and Axin2 gene expression. Based on these studies, we hypothesized that Wnt signaling could inhibit islet cell proliferation because loss of menin function is thought to increase endocrine tumor cell proliferation. TGP61 rodent islet tumor cells treated with a glycogen synthase kinase 3beta inhibitor that increases Wnt pathway signaling had decreased cell proliferation compared with vehicle-treated cells. Collectively, these data suggest that menin has an essential role in Wnt/beta-catenin signaling through a mechanism that eventually affects histone trimethylation of the downstream target gene Axin2, and activation of Wnt/beta-catenin signaling inhibits islet tumor cell proliferation.

Authors+Show Affiliations

David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA 90095-6904, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19074834

Citation

Chen, Gao, et al. "Menin Promotes the Wnt Signaling Pathway in Pancreatic Endocrine Cells." Molecular Cancer Research : MCR, vol. 6, no. 12, 2008, pp. 1894-907.
Chen G, A J, Wang M, et al. Menin promotes the Wnt signaling pathway in pancreatic endocrine cells. Mol Cancer Res. 2008;6(12):1894-907.
Chen, G., A, J., Wang, M., Farley, S., Lee, L. Y., Lee, L. C., & Sawicki, M. P. (2008). Menin promotes the Wnt signaling pathway in pancreatic endocrine cells. Molecular Cancer Research : MCR, 6(12), 1894-907. https://doi.org/10.1158/1541-7786.MCR-07-2206
Chen G, et al. Menin Promotes the Wnt Signaling Pathway in Pancreatic Endocrine Cells. Mol Cancer Res. 2008;6(12):1894-907. PubMed PMID: 19074834.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Menin promotes the Wnt signaling pathway in pancreatic endocrine cells. AU - Chen,Gao, AU - A,Jingbo, AU - Wang,Min, AU - Farley,Steven, AU - Lee,Lung-Yi, AU - Lee,Lung-Ching, AU - Sawicki,Mark P, PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/2/3/medline SP - 1894 EP - 907 JF - Molecular cancer research : MCR JO - Mol Cancer Res VL - 6 IS - 12 N2 - Menin is a tumor suppressor protein mutated in patients with multiple endocrine neoplasia type 1. We show that menin is essential for canonical Wnt/beta-catenin signaling in cultured rodent islet tumor cells. In these cells, overexpression of menin significantly enhances TCF gene assay reporter activity in response to beta-catenin activation. Contrastingly, inhibition of menin expression with Men1 siRNA decreases TCF reporter gene activity. Likewise, multiple endocrine neoplasia type 1 disease associated missense mutations of menin abrogate the ability to increase TCF reporter gene activity. We show that menin physically interacts with proteins involved in the canonical Wnt signaling pathway, including beta-catenin, TCF3 (TCFL1), and weakly with TCF4 (TCFL2). Menin overexpression increases expression of the Wnt/beta-catenin downstream target gene Axin2, which is associated with increased H3K4 trimethylation of the Axin2 gene promoter. Moreover, inhibition of menin expression by siRNA abrogates H3K4 trimethylation and Axin2 gene expression. Based on these studies, we hypothesized that Wnt signaling could inhibit islet cell proliferation because loss of menin function is thought to increase endocrine tumor cell proliferation. TGP61 rodent islet tumor cells treated with a glycogen synthase kinase 3beta inhibitor that increases Wnt pathway signaling had decreased cell proliferation compared with vehicle-treated cells. Collectively, these data suggest that menin has an essential role in Wnt/beta-catenin signaling through a mechanism that eventually affects histone trimethylation of the downstream target gene Axin2, and activation of Wnt/beta-catenin signaling inhibits islet tumor cell proliferation. SN - 1541-7786 UR - https://www.unboundmedicine.com/medline/citation/19074834/Menin_promotes_the_Wnt_signaling_pathway_in_pancreatic_endocrine_cells_ L2 - http://mcr.aacrjournals.org/cgi/pmidlookup?view=long&pmid=19074834 DB - PRIME DP - Unbound Medicine ER -