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Role of a thymic stromal cell clone in inducing the stage-specific differentiation of various subpopulations of double negative thymocytes.
J Immunol. 1991 Aug 15; 147(4):1147-52.JI

Abstract

The monolayer of a thymic stromal cell clone termed MRL104.8a induced the differentiation of adult double negative (DN) thymocytes (CD3-4-8-) through a CD3-4-8+ intermediate into CD3- (or dull) 4+8+ stages. DN thymocytes were separated into three subpopulations depending on their cell-surface expression of Pgp-1 and IL-2R, namely, Pgp-1+IL-2R-, Pgp-1-IL-2R+, and Pgp-1-IL-2R-. The present study investigated the requirements of the MRL104.8a monolayer for inducing the differentiation of these DN thymocyte subpopulations. The following were revealed: i) the MRL104.8a monolayer failed to induce the differentiation of a Pgp-1+IL-2R- subpopulation; ii) whereas a Pgp-1-IL-2R+ subpopulation did not express either CD4 or CD8 Ag when cultured in medium, culturing this subpopulation on the thymic stromal cell monolayers resulted in the expression of CD8 but not CD4 Ag; and iii) a Pgp-1-IL-2R- DN subpopulation obtained through less extensive treatments with anti-CD4 and anti-CD8 antibodies in the presence of C before sorting procedures spontaneously differentiated into double positive cells in medium. In contrast, most of DN cells with the same phenotype obtained through extensive anti-CD4 and -CD8 treatments before sorting failed to express CD4 and/or CD8 Ag in medium but could differentiate through a CD3-4-8+ into more mature stages only when they were cultured on the thymic stromal monolayer. These results indicate differential requirements of thymic stromal cells for the differentiation of various DN subpopulations with qualitatively distinct phenotypes and different magnitudes (very low vs almost zero levels) of CD4/CD8 expression.

Authors+Show Affiliations

Biomedical Research Center, Osaka University Medical School, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1907990

Citation

Nagamine, J, et al. "Role of a Thymic Stromal Cell Clone in Inducing the Stage-specific Differentiation of Various Subpopulations of Double Negative Thymocytes." Journal of Immunology (Baltimore, Md. : 1950), vol. 147, no. 4, 1991, pp. 1147-52.
Nagamine J, Takeda K, Tatsumi Y, et al. Role of a thymic stromal cell clone in inducing the stage-specific differentiation of various subpopulations of double negative thymocytes. J Immunol. 1991;147(4):1147-52.
Nagamine, J., Takeda, K., Tatsumi, Y., Ogata, M., Miyake, K., Hamaoka, T., & Fujiwara, H. (1991). Role of a thymic stromal cell clone in inducing the stage-specific differentiation of various subpopulations of double negative thymocytes. Journal of Immunology (Baltimore, Md. : 1950), 147(4), 1147-52.
Nagamine J, et al. Role of a Thymic Stromal Cell Clone in Inducing the Stage-specific Differentiation of Various Subpopulations of Double Negative Thymocytes. J Immunol. 1991 Aug 15;147(4):1147-52. PubMed PMID: 1907990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of a thymic stromal cell clone in inducing the stage-specific differentiation of various subpopulations of double negative thymocytes. AU - Nagamine,J, AU - Takeda,K, AU - Tatsumi,Y, AU - Ogata,M, AU - Miyake,K, AU - Hamaoka,T, AU - Fujiwara,H, PY - 1991/8/15/pubmed PY - 1991/8/15/medline PY - 1991/8/15/entrez SP - 1147 EP - 52 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 147 IS - 4 N2 - The monolayer of a thymic stromal cell clone termed MRL104.8a induced the differentiation of adult double negative (DN) thymocytes (CD3-4-8-) through a CD3-4-8+ intermediate into CD3- (or dull) 4+8+ stages. DN thymocytes were separated into three subpopulations depending on their cell-surface expression of Pgp-1 and IL-2R, namely, Pgp-1+IL-2R-, Pgp-1-IL-2R+, and Pgp-1-IL-2R-. The present study investigated the requirements of the MRL104.8a monolayer for inducing the differentiation of these DN thymocyte subpopulations. The following were revealed: i) the MRL104.8a monolayer failed to induce the differentiation of a Pgp-1+IL-2R- subpopulation; ii) whereas a Pgp-1-IL-2R+ subpopulation did not express either CD4 or CD8 Ag when cultured in medium, culturing this subpopulation on the thymic stromal cell monolayers resulted in the expression of CD8 but not CD4 Ag; and iii) a Pgp-1-IL-2R- DN subpopulation obtained through less extensive treatments with anti-CD4 and anti-CD8 antibodies in the presence of C before sorting procedures spontaneously differentiated into double positive cells in medium. In contrast, most of DN cells with the same phenotype obtained through extensive anti-CD4 and -CD8 treatments before sorting failed to express CD4 and/or CD8 Ag in medium but could differentiate through a CD3-4-8+ into more mature stages only when they were cultured on the thymic stromal monolayer. These results indicate differential requirements of thymic stromal cells for the differentiation of various DN subpopulations with qualitatively distinct phenotypes and different magnitudes (very low vs almost zero levels) of CD4/CD8 expression. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/1907990/Role_of_a_thymic_stromal_cell_clone_in_inducing_the_stage_specific_differentiation_of_various_subpopulations_of_double_negative_thymocytes_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=1907990 DB - PRIME DP - Unbound Medicine ER -