Tags

Type your tag names separated by a space and hit enter

Optimization of headspace solid phase microextraction procedure for trace analysis of toluene.
Int J Occup Saf Ergon. 2008; 14(4):395-405.IJ

Abstract

This study describes optimization of headspace solid phase microextraction followed by gas chromatography equipped with a flame ionization detector for toluene at trace level in spiked urine. The parameters affecting the extraction and gas chromatographic determination of analytes were studied: extraction time and temperature; desorption time and temperature; addition of NaCl; and pH, volume and agitation of the sample. Optimized headspace extraction was carried out at 30 degrees C for 6 min in the presence of 0.2 g.m(-1)of NaCl in the sample solution. Also, sample volume and sample pH were optimized at 5 ml and 7 (neutral pH), respectively. Desorption of the analytes was carried out at 250 degrees C for 60 s. The optimized procedure was validated with 3 different pools of spiked urine; it showed good reproducibility over 6 consecutive days and 6 within-day experiments. The study also determined the accuracy, linearity and detection limits of this method.

Authors+Show Affiliations

School of Public Health & Institute of Public Health Research, Center for Environmental Research, Tehran University of Medical Sciences. IR Iran. shahtaheri@tums.ac.irNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19080044

Citation

Heidari, Hamid-Reza, et al. "Optimization of Headspace Solid Phase Microextraction Procedure for Trace Analysis of Toluene." International Journal of Occupational Safety and Ergonomics : JOSE, vol. 14, no. 4, 2008, pp. 395-405.
Heidari HR, Shahtaheri SJ, Golbabaei F, et al. Optimization of headspace solid phase microextraction procedure for trace analysis of toluene. Int J Occup Saf Ergon. 2008;14(4):395-405.
Heidari, H. R., Shahtaheri, S. J., Golbabaei, F., Alimohammadi, M., & Rahimi-Froushani, A. (2008). Optimization of headspace solid phase microextraction procedure for trace analysis of toluene. International Journal of Occupational Safety and Ergonomics : JOSE, 14(4), 395-405.
Heidari HR, et al. Optimization of Headspace Solid Phase Microextraction Procedure for Trace Analysis of Toluene. Int J Occup Saf Ergon. 2008;14(4):395-405. PubMed PMID: 19080044.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of headspace solid phase microextraction procedure for trace analysis of toluene. AU - Heidari,Hamid-Reza, AU - Shahtaheri,Seyed Jamaleddin, AU - Golbabaei,Farideh, AU - Alimohammadi,Mahmoud, AU - Rahimi-Froushani,Abbas, PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/2/13/medline SP - 395 EP - 405 JF - International journal of occupational safety and ergonomics : JOSE JO - Int J Occup Saf Ergon VL - 14 IS - 4 N2 - This study describes optimization of headspace solid phase microextraction followed by gas chromatography equipped with a flame ionization detector for toluene at trace level in spiked urine. The parameters affecting the extraction and gas chromatographic determination of analytes were studied: extraction time and temperature; desorption time and temperature; addition of NaCl; and pH, volume and agitation of the sample. Optimized headspace extraction was carried out at 30 degrees C for 6 min in the presence of 0.2 g.m(-1)of NaCl in the sample solution. Also, sample volume and sample pH were optimized at 5 ml and 7 (neutral pH), respectively. Desorption of the analytes was carried out at 250 degrees C for 60 s. The optimized procedure was validated with 3 different pools of spiked urine; it showed good reproducibility over 6 consecutive days and 6 within-day experiments. The study also determined the accuracy, linearity and detection limits of this method. SN - 1080-3548 UR - https://www.unboundmedicine.com/medline/citation/19080044/Optimization_of_headspace_solid_phase_microextraction_procedure_for_trace_analysis_of_toluene_ L2 - https://www.tandfonline.com/doi/full/10.1080/10803548.2008.11076778 DB - PRIME DP - Unbound Medicine ER -