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[Construction of a human phage display ScFv library against Mycobacterium tuberculosis].
Zhonghua Jie He He Hu Xi Za Zhi. 2008 Sep; 31(9):682-5.ZJ

Abstract

OBJECTIVE

To construct a human phage display single-chain Fv (ScFv) antibody library against Mycobacterium tuberculosis (MTB), for specific ScFv antibody cloning.

METHODS

Total RNA was isolated from the lymphocytes of patients with positive serum antibody against MTB and reverse transcribed into cDNA. The heavy chain and light chain variable region gene of human immunoglobulin were amplified individually by PCR and then assembled into ScFv genes. The ScFv genes were ligated into phagemid pCANTAB5S. The human phage display ScFv library against MTB was constructed by transforming the recombinant phagemid into E. coli TG1 with the presence of helper phage M13K07.

RESULTS

The human phage display ScFv library containing 10(7) different clones was constructed successfully.

CONCLUSIONS

A phage display ScFv library against MTB has been constructed based on the variable region gene of immunoglobulin of the lymphocytes of TB patients and phagemid pCANTAB5S. The specific ScFv antibodies can be screened from this library.

Authors+Show Affiliations

Shanghai Key Laboratory of Tuberculosis, Pulmonary Hospital of Shanghai Affiliated Tongji University, Shanghai 200433, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19080570

Citation

Yang, Hua, et al. "[Construction of a Human Phage Display ScFv Library Against Mycobacterium Tuberculosis]." Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, vol. 31, no. 9, 2008, pp. 682-5.
Yang H, Zhou XG, Shi L, et al. [Construction of a human phage display ScFv library against Mycobacterium tuberculosis]. Zhonghua Jie He He Hu Xi Za Zhi. 2008;31(9):682-5.
Yang, H., Zhou, X. G., Shi, L., Feng, Y. H., Qin, L. H., Zheng, R. J., Wang, J., & Hu, Z. Y. (2008). [Construction of a human phage display ScFv library against Mycobacterium tuberculosis]. Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, 31(9), 682-5.
Yang H, et al. [Construction of a Human Phage Display ScFv Library Against Mycobacterium Tuberculosis]. Zhonghua Jie He He Hu Xi Za Zhi. 2008;31(9):682-5. PubMed PMID: 19080570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Construction of a human phage display ScFv library against Mycobacterium tuberculosis]. AU - Yang,Hua, AU - Zhou,Xin-gang, AU - Shi,Li, AU - Feng,Yong-hong, AU - Qin,Lian-hua, AU - Zheng,Rui-juan, AU - Wang,Jie, AU - Hu,Zhong-yi, PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2010/9/10/medline SP - 682 EP - 5 JF - Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases JO - Zhonghua Jie He He Hu Xi Za Zhi VL - 31 IS - 9 N2 - OBJECTIVE: To construct a human phage display single-chain Fv (ScFv) antibody library against Mycobacterium tuberculosis (MTB), for specific ScFv antibody cloning. METHODS: Total RNA was isolated from the lymphocytes of patients with positive serum antibody against MTB and reverse transcribed into cDNA. The heavy chain and light chain variable region gene of human immunoglobulin were amplified individually by PCR and then assembled into ScFv genes. The ScFv genes were ligated into phagemid pCANTAB5S. The human phage display ScFv library against MTB was constructed by transforming the recombinant phagemid into E. coli TG1 with the presence of helper phage M13K07. RESULTS: The human phage display ScFv library containing 10(7) different clones was constructed successfully. CONCLUSIONS: A phage display ScFv library against MTB has been constructed based on the variable region gene of immunoglobulin of the lymphocytes of TB patients and phagemid pCANTAB5S. The specific ScFv antibodies can be screened from this library. SN - 1001-0939 UR - https://www.unboundmedicine.com/medline/citation/19080570/[Construction_of_a_human_phage_display_ScFv_library_against_Mycobacterium_tuberculosis]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=1001-0939&year=2008&vol=31&issue=9&fpage=682 DB - PRIME DP - Unbound Medicine ER -