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In vitro susceptibility of bovine digital dermatitis associated spirochaetes to antimicrobial agents.
Vet Microbiol. 2009 Apr 14; 136(1-2):115-20.VM

Abstract

Bovine digital dermatitis (BDD) is an infectious lameness in cattle, which has a large global impact in terms of animal welfare and cost. The majority of evidence suggests that spirochaetes are the aetiological agent of this disease. The aim of this study was to identify the susceptibility of BDD associated spirochaetes to a range of antimicrobial agents with a view to potential usage in vivo to treat this widespread cattle disease. A microdilution method was adapted to determine the in vitro susceptibilities of 19 UK digital dermatitis spirochaetes (6 Treponema medium/Treponema vincentii-like, 8 Treponema phagedenis-like and 5 Treponema denticola/Treponema putidum-like) to eight relevant antimicrobials. The BDD spirochaetes exhibited the highest susceptibility to penicillin and erythromycin and this information may now be used to aid development of efficacious treatments. This study has also identified that BDD spirochaete T167 is spectinomycin resistant and that the likely biological basis is a point mutation in the 16S rRNA gene. Interestingly, nearly all Brachyspira isolate 16S rRNA gene sequences in Genbank have this substitution, suggesting it may be responsible for the characteristic spectinomycin resistance reported for the Brachyspira genus.

Authors+Show Affiliations

Department of Veterinary Pathology, University of Liverpool, Liverpool L69 CBX, UK. evansnj@liverpool.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19081208

Citation

Evans, Nicholas J., et al. "In Vitro Susceptibility of Bovine Digital Dermatitis Associated Spirochaetes to Antimicrobial Agents." Veterinary Microbiology, vol. 136, no. 1-2, 2009, pp. 115-20.
Evans NJ, Brown JM, Demirkan I, et al. In vitro susceptibility of bovine digital dermatitis associated spirochaetes to antimicrobial agents. Vet Microbiol. 2009;136(1-2):115-20.
Evans, N. J., Brown, J. M., Demirkan, I., Birtles, R., Hart, C. A., & Carter, S. D. (2009). In vitro susceptibility of bovine digital dermatitis associated spirochaetes to antimicrobial agents. Veterinary Microbiology, 136(1-2), 115-20. https://doi.org/10.1016/j.vetmic.2008.10.015
Evans NJ, et al. In Vitro Susceptibility of Bovine Digital Dermatitis Associated Spirochaetes to Antimicrobial Agents. Vet Microbiol. 2009 Apr 14;136(1-2):115-20. PubMed PMID: 19081208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro susceptibility of bovine digital dermatitis associated spirochaetes to antimicrobial agents. AU - Evans,Nicholas J, AU - Brown,Jennifer M, AU - Demirkan,Ibrahim, AU - Birtles,Richard, AU - Hart,C Anthony, AU - Carter,Stuart D, Y1 - 2008/10/30/ PY - 2008/06/20/received PY - 2008/10/13/revised PY - 2008/10/20/accepted PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/7/22/medline SP - 115 EP - 20 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 136 IS - 1-2 N2 - Bovine digital dermatitis (BDD) is an infectious lameness in cattle, which has a large global impact in terms of animal welfare and cost. The majority of evidence suggests that spirochaetes are the aetiological agent of this disease. The aim of this study was to identify the susceptibility of BDD associated spirochaetes to a range of antimicrobial agents with a view to potential usage in vivo to treat this widespread cattle disease. A microdilution method was adapted to determine the in vitro susceptibilities of 19 UK digital dermatitis spirochaetes (6 Treponema medium/Treponema vincentii-like, 8 Treponema phagedenis-like and 5 Treponema denticola/Treponema putidum-like) to eight relevant antimicrobials. The BDD spirochaetes exhibited the highest susceptibility to penicillin and erythromycin and this information may now be used to aid development of efficacious treatments. This study has also identified that BDD spirochaete T167 is spectinomycin resistant and that the likely biological basis is a point mutation in the 16S rRNA gene. Interestingly, nearly all Brachyspira isolate 16S rRNA gene sequences in Genbank have this substitution, suggesting it may be responsible for the characteristic spectinomycin resistance reported for the Brachyspira genus. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/19081208/In_vitro_susceptibility_of_bovine_digital_dermatitis_associated_spirochaetes_to_antimicrobial_agents_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(08)00491-4 DB - PRIME DP - Unbound Medicine ER -