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Comparison of murine thymic stromal lymphopoietin- and polyinosinic polycytidylic acid-mediated placental dendritic cell activation.
J Reprod Immunol. 2009 Jan; 79(2):119-28.JR

Abstract

We confirmed previously the existence of thymic stromal lymphopoietin (TSLP)-positive cells in murine placenta by flow cytometry. To compare the characteristics of Toll-like receptor 3 (TLR3)- and TSLP-mediated placental dendritic cell (DC) activation, pregnant BALB/c mouse mated with C57BL/6 male were used as a model of allogenic gestation. Placental CD11c(+) DCs were potently activated by the TLR3-agonist polyinosinic polycytidylic acid [poly (I:C)], subsequently causing increased expression of co-stimulatory molecules. Accordingly, increased intracellular production of interleukin (IL)-12 and interferon (IFN)-gamma, but not IL-4 or IL-10, were detected after stimulation by poly (I:C). In the case of TSLP-stimulation, although increased expression of co-stimulatory molecules was also detected, there was no substantial increase of intracellular production of IL-12, IFN-gamma, IL-4 or IL-10. In contrast, the expression of the Th2 cell-attracting chemokine, the thymus and activation-regulated chemokine (TARC) or CCL17, was significantly boosted in response to TSLP induction, whereas no significant increase of CCL17 was observed when triggering TLR3 with its specific agonist poly (I:C). The data were further supported by a CD4(+)IL-10(+) cell migratory assay. These results suggest that TSLP-TSLP receptor interaction may result in a Th2-type microenvironment at the feto-maternal interface by inducing the production of Th2 cell-attracting chemokine and modulating the immigration of Th2-type cells.

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Authors+Show Affiliations

Lin Y
Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. yilinonline@gmail.com
Wang W
No affiliation info available
Jin H
No affiliation info available
Zhong Y
No affiliation info available
Di J
No affiliation info available
Zeng S
No affiliation info available
Saito S
No affiliation info available

MeSH

AnimalsAntibodiesCell MovementCells, CulturedCytokinesDendritic CellsFemaleMaleMicePlacentaPoly I-CT-Lymphocytes

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19081639

Citation

Lin, Yi, et al. "Comparison of Murine Thymic Stromal Lymphopoietin- and Polyinosinic Polycytidylic Acid-mediated Placental Dendritic Cell Activation." Journal of Reproductive Immunology, vol. 79, no. 2, 2009, pp. 119-28.
Lin Y, Wang W, Jin H, et al. Comparison of murine thymic stromal lymphopoietin- and polyinosinic polycytidylic acid-mediated placental dendritic cell activation. J Reprod Immunol. 2009;79(2):119-28.
Lin, Y., Wang, W., Jin, H., Zhong, Y., Di, J., Zeng, S., & Saito, S. (2009). Comparison of murine thymic stromal lymphopoietin- and polyinosinic polycytidylic acid-mediated placental dendritic cell activation. Journal of Reproductive Immunology, 79(2), 119-28. https://doi.org/10.1016/j.jri.2008.10.001
Lin Y, et al. Comparison of Murine Thymic Stromal Lymphopoietin- and Polyinosinic Polycytidylic Acid-mediated Placental Dendritic Cell Activation. J Reprod Immunol. 2009;79(2):119-28. PubMed PMID: 19081639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of murine thymic stromal lymphopoietin- and polyinosinic polycytidylic acid-mediated placental dendritic cell activation. AU - Lin,Yi, AU - Wang,Wenjing, AU - Jin,Haiyan, AU - Zhong,Yanmin, AU - Di,Jingfang, AU - Zeng,Shan, AU - Saito,Shigeru, Y1 - 2008/12/10/ PY - 2007/11/03/received PY - 2008/10/19/revised PY - 2008/10/22/accepted PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/9/4/medline SP - 119 EP - 28 JF - Journal of reproductive immunology JO - J. Reprod. Immunol. VL - 79 IS - 2 N2 - We confirmed previously the existence of thymic stromal lymphopoietin (TSLP)-positive cells in murine placenta by flow cytometry. To compare the characteristics of Toll-like receptor 3 (TLR3)- and TSLP-mediated placental dendritic cell (DC) activation, pregnant BALB/c mouse mated with C57BL/6 male were used as a model of allogenic gestation. Placental CD11c(+) DCs were potently activated by the TLR3-agonist polyinosinic polycytidylic acid [poly (I:C)], subsequently causing increased expression of co-stimulatory molecules. Accordingly, increased intracellular production of interleukin (IL)-12 and interferon (IFN)-gamma, but not IL-4 or IL-10, were detected after stimulation by poly (I:C). In the case of TSLP-stimulation, although increased expression of co-stimulatory molecules was also detected, there was no substantial increase of intracellular production of IL-12, IFN-gamma, IL-4 or IL-10. In contrast, the expression of the Th2 cell-attracting chemokine, the thymus and activation-regulated chemokine (TARC) or CCL17, was significantly boosted in response to TSLP induction, whereas no significant increase of CCL17 was observed when triggering TLR3 with its specific agonist poly (I:C). The data were further supported by a CD4(+)IL-10(+) cell migratory assay. These results suggest that TSLP-TSLP receptor interaction may result in a Th2-type microenvironment at the feto-maternal interface by inducing the production of Th2 cell-attracting chemokine and modulating the immigration of Th2-type cells. SN - 1872-7603 UR - https://www.unboundmedicine.com/medline/citation/19081639/Comparison_of_murine_thymic_stromal_lymphopoietin__and_polyinosinic_polycytidylic_acid_mediated_placental_dendritic_cell_activation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-0378(08)00098-3 DB - PRIME DP - Unbound Medicine ER -