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Electrochemical studies on the oxidation of guanine and adenine at cyclodextrin modified electrodes.
Analyst. 2008 Dec; 133(12):1664-72.A

Abstract

An electrochemical sensor for guanine and adenine using cyclodextrin-modified poly(N-acetylaniline) (PNAANI) on a carbon paste electrode has been developed. The oxidation mechanism of guanine and adenine on the surface of the electrode was investigated by cyclic voltammetry. It was found that the electrode processes are irreversible, pH dependent, and involve several reaction products. The electron transfer process occurs in consecutive steps with the formation of a strongly adsorbed intermediate on the electrode surface. Also, a new method for estimating the apparent formation constants of guanine and adenine with the immobilized cyclodextrins, through the change of surface coverage of studied analytes has been reported. Both guanine and adenine showed linear concentrations in the range of 0.1-10 microM by using differential pulse voltammetry, with an experimental limit of detection down to 0.05 microM. Linear concentration ranges of 2-150 microM for guanine and 6-104 microM for adenine have been found when cyclic voltammetry was used for determination of both analytes.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Abbaspour A
Chemistry Department, College of Sciences, Shiraz University, Shiraz, Iran. abbaspour@chem.susc.ac.ir
Noori A
No affiliation info available

MeSH

AdenineAdsorptionCarbonCyclodextrinsElectrochemistryGuanineHydrogen-Ion ConcentrationMicroelectrodesOxidation-Reduction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19082068

Citation

Abbaspour, Abdolkarim, and Abolhassan Noori. "Electrochemical Studies On the Oxidation of Guanine and Adenine at Cyclodextrin Modified Electrodes." The Analyst, vol. 133, no. 12, 2008, pp. 1664-72.
Abbaspour A, Noori A. Electrochemical studies on the oxidation of guanine and adenine at cyclodextrin modified electrodes. Analyst. 2008;133(12):1664-72.
Abbaspour, A., & Noori, A. (2008). Electrochemical studies on the oxidation of guanine and adenine at cyclodextrin modified electrodes. The Analyst, 133(12), 1664-72. https://doi.org/10.1039/b806920d
Abbaspour A, Noori A. Electrochemical Studies On the Oxidation of Guanine and Adenine at Cyclodextrin Modified Electrodes. Analyst. 2008;133(12):1664-72. PubMed PMID: 19082068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemical studies on the oxidation of guanine and adenine at cyclodextrin modified electrodes. AU - Abbaspour,Abdolkarim, AU - Noori,Abolhassan, Y1 - 2008/09/02/ PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/6/12/medline SP - 1664 EP - 72 JF - The Analyst JO - Analyst VL - 133 IS - 12 N2 - An electrochemical sensor for guanine and adenine using cyclodextrin-modified poly(N-acetylaniline) (PNAANI) on a carbon paste electrode has been developed. The oxidation mechanism of guanine and adenine on the surface of the electrode was investigated by cyclic voltammetry. It was found that the electrode processes are irreversible, pH dependent, and involve several reaction products. The electron transfer process occurs in consecutive steps with the formation of a strongly adsorbed intermediate on the electrode surface. Also, a new method for estimating the apparent formation constants of guanine and adenine with the immobilized cyclodextrins, through the change of surface coverage of studied analytes has been reported. Both guanine and adenine showed linear concentrations in the range of 0.1-10 microM by using differential pulse voltammetry, with an experimental limit of detection down to 0.05 microM. Linear concentration ranges of 2-150 microM for guanine and 6-104 microM for adenine have been found when cyclic voltammetry was used for determination of both analytes. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/19082068/Electrochemical_studies_on_the_oxidation_of_guanine_and_adenine_at_cyclodextrin_modified_electrodes_ L2 - https://doi.org/10.1039/b806920d DB - PRIME DP - Unbound Medicine ER -