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Hexose transporter expression and function in mouse small intestine: role of diurnal rhythm.
J Gastrointest Surg 2009; 13(4):634-41JG

Abstract

BACKGROUND

Expression and function of hexose transporters vary diurnally in rat small intestine; however, this subject remains unexplored in mice.

AIM

The aim of the study was to investigate the diurnal expression and function of hexose transporters SGLT1, GLUT2, and GLUT5 in mouse small bowel.

METHODS

Twenty-four c57bl6 mice maintained in a 12-h light/dark room (6 AM: -6 PM:) were sacrificed at 9 AM: , 3 PM: , 9 PM: , and 3 AM: (n = 6 each). In duodenal, jejunal, and ileal mucosa, total cellular mRNA and protein levels were quantitated by real-time PCR and semiquantitative Western blotting, respectively. The everted sleeve technique measured transporter-mediated glucose uptake at 9 AM: and 9 PM: .

RESULTS

mRNA expression of SGLT1, GLUT2, and GLUT5 varied diurnally in all three intestinal segments (p <or= 0.03). SGLT1, GLUT2, and GLUT5 protein levels varied diurnally in duodenum and jejunum (p < 0.05) but not in ileum. Transporter-mediated glucose uptake was greater at 9 PM: than 9 AM: (p <or= 0.04) in all three segments. V (max) was greater in duodenum (10 vs 6 nmol/cm/s) and jejunum (8 vs 5 nmol/cm/s) at 9 PM: compared to 9 AM: (p = 0.01); K (m) remained unchanged. mRNA levels of intestinal hexose transporters varied diurnally. Protein levels peaked 6-12 h later during dark cycle when >70% of food intake occurred; glucose transport followed a similar pattern with increased uptake at 9 PM: .

CONCLUSION

Hexose transporter expression and function vary diurnally with nocturnal feeding patterns of mice.

Authors+Show Affiliations

Gastroenterology Research Unit (GU 1001), Department of Surgery, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA. fatima.javairiah@mayo.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19082670

Citation

Fatima, Javairiah, et al. "Hexose Transporter Expression and Function in Mouse Small Intestine: Role of Diurnal Rhythm." Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract, vol. 13, no. 4, 2009, pp. 634-41.
Fatima J, Iqbal CW, Houghton SG, et al. Hexose transporter expression and function in mouse small intestine: role of diurnal rhythm. J Gastrointest Surg. 2009;13(4):634-41.
Fatima, J., Iqbal, C. W., Houghton, S. G., Kasparek, M. S., Duenes, J. A., Zheng, Y., & Sarr, M. G. (2009). Hexose transporter expression and function in mouse small intestine: role of diurnal rhythm. Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract, 13(4), pp. 634-41. doi:10.1007/s11605-008-0776-4.
Fatima J, et al. Hexose Transporter Expression and Function in Mouse Small Intestine: Role of Diurnal Rhythm. J Gastrointest Surg. 2009;13(4):634-41. PubMed PMID: 19082670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hexose transporter expression and function in mouse small intestine: role of diurnal rhythm. AU - Fatima,Javairiah, AU - Iqbal,Corey W, AU - Houghton,Scott G, AU - Kasparek,Michael S, AU - Duenes,Judith A, AU - Zheng,Ye, AU - Sarr,Michael G, Y1 - 2008/12/11/ PY - 2008/05/08/received PY - 2008/11/24/accepted PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/9/23/medline SP - 634 EP - 41 JF - Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract JO - J. Gastrointest. Surg. VL - 13 IS - 4 N2 - BACKGROUND: Expression and function of hexose transporters vary diurnally in rat small intestine; however, this subject remains unexplored in mice. AIM: The aim of the study was to investigate the diurnal expression and function of hexose transporters SGLT1, GLUT2, and GLUT5 in mouse small bowel. METHODS: Twenty-four c57bl6 mice maintained in a 12-h light/dark room (6 AM: -6 PM:) were sacrificed at 9 AM: , 3 PM: , 9 PM: , and 3 AM: (n = 6 each). In duodenal, jejunal, and ileal mucosa, total cellular mRNA and protein levels were quantitated by real-time PCR and semiquantitative Western blotting, respectively. The everted sleeve technique measured transporter-mediated glucose uptake at 9 AM: and 9 PM: . RESULTS: mRNA expression of SGLT1, GLUT2, and GLUT5 varied diurnally in all three intestinal segments (p <or= 0.03). SGLT1, GLUT2, and GLUT5 protein levels varied diurnally in duodenum and jejunum (p < 0.05) but not in ileum. Transporter-mediated glucose uptake was greater at 9 PM: than 9 AM: (p <or= 0.04) in all three segments. V (max) was greater in duodenum (10 vs 6 nmol/cm/s) and jejunum (8 vs 5 nmol/cm/s) at 9 PM: compared to 9 AM: (p = 0.01); K (m) remained unchanged. mRNA levels of intestinal hexose transporters varied diurnally. Protein levels peaked 6-12 h later during dark cycle when >70% of food intake occurred; glucose transport followed a similar pattern with increased uptake at 9 PM: . CONCLUSION: Hexose transporter expression and function vary diurnally with nocturnal feeding patterns of mice. SN - 1873-4626 UR - https://www.unboundmedicine.com/medline/citation/19082670/Hexose_transporter_expression_and_function_in_mouse_small_intestine:_role_of_diurnal_rhythm_ L2 - https://dx.doi.org/10.1007/s11605-008-0776-4 DB - PRIME DP - Unbound Medicine ER -